Peptide hormones such as ANG II and endothelin contribute to cardiac remodeling after myocardial infarction by stimulating myocyte hypertrophy and myofibroblast proliferation. In contrast, angiotensin-(1-7) [ANG-(1-7)] infusion after myocardial infarction reduced myocyte size and attenuated ventricular dysfunction and remodeling. We measured the effect of ANG-(1-7) on protein and DNA synthesis in cultured neonatal rat myocytes to assess the role of the heptapeptide in cell growth. ANG-(1-7) significantly attenuated either fetal bovine serum-or endothelin-1-stimulated [3 H]leucine incorporation into myocytes with no effect on, the selective ANG type 1-7 (AT1-7) receptor antagonist, blocked the ANG-(1-7)-mediated reduction in protein synthesis in cardiac myocytes, whereas the AT1 and AT2 angiotensin peptide receptors were ineffective. Serum-stimulated ERK1/ERK2 mitogen-activated protein kinase activity was significantly decreased by ANG-(1-7) in myocytes, a response that was also blocked by ]-ANG-(1-7). Both rat heart and cardiac myocytes express the mRNA for the mas receptor, and a 59-kDa immunoreactive protein was identified in both extracts of rat heart and cultured myocytes by Western blot hybridization with the use of an antibody to mas, an ANG-(1-7) receptor. Transfection of cultured myocytes with an antisense oligonucleotide to the mas receptor blocked the ANG-(1-7)-mediated inhibition of serum-stimulated MAPK activation, whereas a sense oligonucleotide was ineffective. These results suggest that ANG-(1-7) reduces the growth of cardiomyocytes through activation of the mas receptor. Because ANG-(1-7) is elevated after treatment with angiotensin-converting enzyme inhibitors or AT1 receptor blockers, ANG-(1-7) may contribute to their beneficial effects on cardiac dysfunction and ventricular remodeling after myocardial infarction. cardiac hypertrophy; mitogen-activated protein kinases [ANG-(1-7)] is an endogenous peptide hormone that produces unique physiological responses that are often opposite to those of the well-characterized angiotensin peptide ANG II (15). The ability of ANG II to increase blood pressure is documented; it is a potent vasoconstrictor, it stimulates thirst and aldosterone release, and inhibition of its production or effect with the use of angiotensin-converting enzyme (ACE) inhibitors or ANG type 1 (AT 1 ) receptor antagonists reduces mean arterial pressure (45). In addition, ANG II stimulates vascular growth as well as hypertrophy in terminally differentiated cells. In contrast, ANG-(1-7) reduces the blood pressure of hypertensive dogs and rats (4, 33), alters renal fluid absorption (11,19,22), causes vasodilation (6, 34, 35), and participates in the antihypertensive responses to ACE inhibition or AT 1 receptor blockade in hypertensive rats (24,25). In addition, we showed that ANG-(1-7) reduces vascular growth in vitro and in vivo (17,42,44), suggesting that ANG-(1-7) may act as an endogenous regulator of cell growth. Thus ANG-(1-7) opposes both the pressor and proliferative effects of ...