Endothelin in normal lung tissue of newborn mammals: immunocytochemical distribution and co-localization with serotonin and calcitonin gene-related peptide.

Seldeslagh, Kristine A.; Lauweryns, Joseph M.

doi:10.1177/41.10.8245408

Cited by 23 publications

(7 citation statements)

References 31 publications

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“…As a first step in studying the phylogeny of the NE (paraneuronal) cells of the fish gill, results from our laboratory indicated that many bioactive substances, including endothelin, are also typical markers of the NE cell system of mammalian airways (Dey et al, 1981;Cutz et al, 1984;Dey & Hoffpanir, 1986;Lauweryns et al, 1986;Seldeslagh & Lauweryns, 1993;Zaccone et al, 1996Zaccone et al, , 1998. Specific cytokeratins and calcium-binding proteins (CaBPs) are also assumed to be possible markers of these cells.…”

Section: Introductionmentioning

confidence: 92%

Immunoreactivity to calcium‐binding proteins (CaBPs) in the epithelia of skin and gill of the catfish,Heteropneustes fossilis

Fasulo

Mauceri

Tagliafierro

et al. 1998

Italian Journal of Zoology

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The skin and gill epithelia of Heteropneustes fossilis have been shown to possess specific patterns of immunoreactivity for the antisera against a set of calcium-binding proteins such as calmodulin, parvalbumin, S-100 protein, and calbindin D28K. In the epidermis, both the more superficial cells and the club cells reacted positively for all the calcium-binding proteins tested, except for calbindin D28K in the former and for parvalbumin in the latter. As in the club cells of the epidermis, also in the gill all the calciumbinding protein antisera, except anti-parvalbumin, appear to stain neuroendocrine cells scattered throughout these epithelia. The colocalization of a serotonin reactivity in the S-100 protein positive cells confirms the qualification of these branchial cells as being of the neuroendocrine type.

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Section: Introductionmentioning

confidence: 92%

Immunoreactivity to calcium‐binding proteins (CaBPs) in the epithelia of skin and gill of the catfish,Heteropneustes fossilis

Fasulo

Mauceri

Tagliafierro

et al. 1998

Italian Journal of Zoology

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“…To our knowledge this peptide is reported for the first time in the diffuse neuroendocrine system of the respiratory organs of lower vertebrates. However, endothelin-like immunoreactivity was previously reported in NEBs of mammalian lung (Seldeslagh and Lauweryns, 1993).…”

Section: Neuroendocrine Markersmentioning

confidence: 88%

Paraneurons in the gills and airways of fishes

Zaccone

Fasulo

Ainis

et al. 1997

Microsc. Res. Tech.

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This chapter describes the distributional patterns of the neuroendocrine cells in the respiratory surfaces of fishes and their bioactive secretions which are compared with similar elements in higher vertebrates. The neuroendocrine cells in the airways of fishes differentiate as solitary and clustered cells, but the clusters are not converted into neuroepithelial bodies which are reported in terrestrial vertebrates. The dipnoan fish Protopterus has innervated neuroendocrine cells in the pneumatic duct region. In Polypterus and Amia the lungs have neuroendocrine cells that are apparently not innervated. Two types of neuroendocrine cells are found in the gill of teleost fishes. These cells are very different by their location, structure and immunohistochemistry. Advanced studies on functional morphology of neuroendocrine cells in fish airways are still necessary to increase our understanding of their multifunctional role in the gill area. Microsc. Res. Tech. 37:4–12, 1997. © 1997 Wiley‐Liss, Inc.

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“…Furthermore, an often underestimated reason for such discrepancies might derive from crossreacting antibodies. Accordingly, only few authors are critical concerning specificity of ET antibodies (Saito et al 1991;Seldeslagh and Lauweryns 1993;Svane et al 1993). For instance, Yanagisawa's group (Saito et al 1991) discussed their results with great care: "since the antibodies might have crossreacted with unknown substances, the immunoreactive material was termed 'endothelin-like' in the present study."…”

Section: Introductionmentioning

confidence: 95%

Endothelin immunocytochemistry: indications of false-positive labeling patterns and non-detectable antigen concentrations

Wolf

Weis

Scheidt

2001

Histochem Cell Biol

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Endothelin is an endothelium-derived peptide with potent vasoconstrictor and mitogenic properties. Since studies concerning the immunocytochemical localization of endothelin are often inconsistent we tried to clear up some of these discrepancies by comparing specificity and labeling patterns of different endothelin antibodies. Monoclonal and polyclonal endothelin antibodies ( n=7) were examined concerning their reactivity with endothelins and heart tissues by immunoblotting. Using immunofluorescence microscopy reactivities with human non-failing hearts, failing hearts, and cultured endothelial cells were examined. Specificity of labelings was assessed by absorption controls and functional controls using endothelial cells conditioned to produce different amounts of endothelin. As shown by immunoblotting five out of seven endothelin antibodies revealed both specific endothelin reactivity and negligible non-specific reactivities with heart proteins. Immunocytochemistry showed vascular reactivity of N-terminal endothelin antibodies to be associated with alpha-smooth muscle actin expression. One N-terminal antibody showed additional nuclear reactivity, and one C-terminal antibody vimentin-like labeling patterns. Although these reactivities were abolished in absorption controls these labelings had to be graded non-specific because functional specificity of endothelin antibodies could not be proven. The remaining antibodies revealed no endothelial reactivity even after tyramide signal amplification. Cellular endothelin concentrations ranged around 2,000-fold below the detection limit of immunocytochemical methods. Discrepancies in endothelin immunocytochemistry may originate from false-positive results and from expression levels of endothelin below the detection limit of immunocytochemical methods.

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Endothelin in normal lung tissue of newborn mammals: immunocytochemical distribution and co-localization with serotonin and calcitonin gene-related peptide.

Cited by 23 publications

References 31 publications

Immunoreactivity to calcium‐binding proteins (CaBPs) in the epithelia of skin and gill of the catfish,Heteropneustes fossilis

Immunoreactivity to calcium‐binding proteins (CaBPs) in the epithelia of skin and gill of the catfish,Heteropneustes fossilis

Paraneurons in the gills and airways of fishes

Endothelin immunocytochemistry: indications of false-positive labeling patterns and non-detectable antigen concentrations

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