Endothelium-dependent and endothelium-independent vasorelaxant effects of tiliacorinine 12′-O-acetate and mechanisms on isolated rat aorta

Tiliacora triandra extract and its major constituent attenuates diabetic kidney and testicular impairment by modulating redox imbalance and pro‐inflammatory responses in rats

“…The plant has been used traditionally for many centuries for treating malaria, diabetes and as a rejuvenating and neurotonic agent. A number of studies have demonstrated the therapeutic effects of T. triandra and its constituents including antimalarial, antimycobacterial, anti‐diabetes, neuroprotection, antioxidant, anticancer, antimicrobial and antihypertensive effect 19–21 . Our earlier study on the chemical constituents from the ethanol extract of T. triandra indicated that fatty acid derivatives isolated from the plant showed inhibitory effect against α‐amylase and α‐glucosidase enzymes and also displayed beneficial effects against experimental induced animal models of diabetes by attenuating hyperglycemia, hyperlipidemia, improving liver and kidney functions as well as enhancing insulin secretion and beta cell function 19,22,23 .…”

“…A number of studies have demonstrated the therapeutic effects of T. triandra and its constituents including antimalarial, antimycobacterial, anti-diabetes, neuroprotection, antioxidant, anticancer, antimicrobial and antihypertensive effect. [19][20][21] Our earlier study on the chemical constituents from the ethanol extract of T. triandra indicated that fatty acid derivatives isolated from the plant showed inhibitory effect against ⊍-amylase and ⊍-glucosidase enzymes and also displayed beneficial effects against experimental induced animal models of diabetes by attenuating hyperglycemia, hyperlipidemia, improving liver and kidney functions as well as enhancing insulin secretion and beta cell function. 19,22,23 Despite the considerable efficacy of T. triandra or its active constituent on diabetes, the effect of the plant extracts of its component on diabetes induced kidney or testicular damage remains unknown.…”

[Pyr1]apelin-13 relaxes the rat thoracic aorta via APJ, NO, AMPK, and potassium channels

“…Krebs solution in the chambers is renewed every 15 min. Phenylephrine (10 −5 M) was used to stimulate vascular contraction (Panthiyaa et al 2019). [Pyr1]apelin-13 was applied when vascular tension reached the plateau phase.…”

“…The vessels for which a sufficient contractile response couldn't be obtained with phenylephrine were excluded from the study. The aortic rings were challenged with 10 −5 M of acetylcholine, and if the vasorelaxant response was greater than 90% of the phenylephrine-induced contraction, endothelium of the aortic rings was considered intact (E+) (Panthiyaa et al 2019). The endothelium was mechanically removed from some aortic rings by gentle rubbing of the intimal surface with a wooden stick (Wang et al 2015).…”

“…The endothelium was mechanically removed from some aortic rings by gentle rubbing of the intimal surface with a wooden stick (Wang et al 2015). Endothelium-denuded (E−) rings were considered to have less than 10% relaxation response of phenylephrine-induced contraction to 10 −5 M acetylcholine (Panthiyaa et al 2019).…”