Endothelium-Dependent Vasorelaxation Induced by Black Currant Concentrate in Rat Thoracic Aorta

Nakamura, Yuko; Matsumoto, Hitoshi; Todoki, Kazuo

doi:10.1254/jjp.89.29

Cited by 73 publications

(42 citation statements)

References 28 publications

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“…Results of in vitro studies with individual anthocyanidin compounds (sugar free molecules, anthocyanin aglycones) were contradictory, as only delphinidin, but not malvidin or cyanidin, caused endotheliumdependent relaxation of rat aortic rings. 22 The study by Nakamura et al 23 showed that four individual anthocyanin compounds, applied either alone or in combination, caused …”

Section: Discussionmentioning

confidence: 99%

Antioxidant and Vasodilatory Effects of Blackberry and Grape Wines

Mudnić

Budimir²,

Modun³

et al. 2012

Journal of Medicinal Food

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In contrast to the well-described various biological effects of grape wines, the potential effects of commonly consumed blackberry wine have not been studied. We examined in vitro antioxidant and vasodilatory effects of four blackberry wines and compared them with the effects of two red and two white grape wines. Although some blackberry wines had lower total phenolic content relative to the red grape wines, their antioxidant capacity was stronger, which may be related to a higher content of non-flavonoid compounds (most notably gallic acid) in blackberry wines. Although maximal vasodilation induced by blackberry wines was generally similar to that of red wines, blackberry wines were less potent vasodilators. Vasodilatory activity of all wines, in addition to their flavonoid and total phenolic content, was most significantly associated with their content of anthocyanins. No association of vasodilation with any individual polyphenolic compound was found. Our results indicate the biological potential of blackberry wines, which deserves deeper scientific attention.

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Section: Discussionmentioning

confidence: 99%

Antioxidant and Vasodilatory Effects of Blackberry and Grape Wines

Mudnić

Budimir²,

Modun³

et al. 2012

Journal of Medicinal Food

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“…Ex vivo aortic ring assays were performed in accordance with the previously described methods of Nakamura et al (24 ). Briefly, male Sprague-Dawley rats (180 -220 g) were killed and the thoracic aortas were then isolated and cut into rings.…”

Section: Ex Vivo Aortic Ring Experimentsmentioning

confidence: 99%

“…We further examined the role of the NO-cGMP pathway in mediating the effect of anthocyanins on vascular dilation in vitro by using a well-established rat aortic ring model (24 ). The relaxant effects of anthocyanins upon phenylephrine-precontracted aortic rings with a functional endothelium were found to be significantly augmented when the cumulative anthocyanin concentration reached 5 g/mL (P Ͻ 0.001; Fig.…”

Section: Vascular Relaxant Effects Of Anthocyanin In Vitromentioning

confidence: 99%

Purified Anthocyanin Supplementation Improves Endothelial Function via NO-cGMP Activation in Hypercholesterolemic Individuals

et al. 2011

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BACKGROUND Anthocyanins have been shown to improve endothelial function in animal models. However, whether these compounds have similar beneficial effects in humans is largely unknown. METHODS In a short-term crossover study, 12 hypercholesterolemic individuals were given oral anthocyanins (320 mg) isolated from berries or placebo. Brachial artery flow-mediated dilation (FMD) was assessed before and after the intervention. In a long-term intervention trial (12 weeks), 150 hypercholesterolemic individuals were given anthocyanins (320 mg/day, n = 75) or placebo (n = 75), after which we measured FMD, plasma cGMP, and other serum biomarkers. Another short-term intervention was conducted in the presence of NO-cGMP inhibitors in 6 people and in a rat aortic ring model (n = 8). RESULTS Significant increases of FMD from 8.3% (0.6%) at baseline to 11.0% (0.8%) at 1 h and 10.1% (0.9%) at 2 h were observed after short-term anthocyanin consumption, concomitantly with increases of plasma anthocyanin concentrations (P < 0.05). In the study participants who received long-term anthocyanin intervention, compared with the control group, we observed significant increases in the FMD (28.4% vs 2.2%), cGMP (12.6% vs −1.2%), and HDL-cholesterol concentrations, but decreases in the serum soluble vascular adhesion molecule-1 and LDL cholesterol concentrations (P < 0.05). The changes in the cGMP and HDL cholesterol concentrations positively correlated with FMD in the anthocyanin group (P < 0.05). In the presence of NO-cGMP inhibitors, the effects of anthocyanin on endothelial function were abolished in human participants and in a rat aortic ring model. CONCLUSIONS Anthocyanin supplementation improves endothelium-dependent vasodilation in hypercholesterolemic individuals. This effect involves activation of the NO-cGMP signaling pathway, improvements in the serum lipid profile, and decreased inflammation.

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“…7,8) Lack of functional vascular endothelium was confirmed by the loss of relaxant response to 3 mM acetylcholine before the experiment began. 9) After the contraction had reached a stable plateau, cumulative concentrations of test compound were added.…”

Section: Establishment Of Concentration-contractile Response Curves Omentioning

confidence: 99%

Vasodilatation Produced by Orientin and Its Mechanism Study

Wang

et al. 2005

Biological & Pharmaceutical Bulletin

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Bamboo leaves, Phyllostachys nigra (LODD. ex. lind-1 MUNRO. L), have been used as a Chinese medicament for thousands of years. Theses leaves have anti-free radical activity and are comparable with the leaf of Ginkgo bilabo, which is one potential resource for natural antioxidant and free radical scavenger. 1) Our previous investigation revealed that bamboo leaves have significant effects on protecting myocardial ischemia in rat, restraining blood platelet aggregation of rabbit, increasing coronal flow and have a vasorelaxant effect on rabbit thoracic aortic rings, and also reduce arterial blood pressure. Here, we further investigated the vasorelaxant effect of four flavonoid C-glycosyl compounds ( Fig. 1): Orientin (Luteolin-8-C-glucoside), Isoorientin (Luteolin-6-Cglucoside), Isovitexin (Apigenin-6-C-glucoside), and Vitexin (Apigenin-8-C-glucoside), first isolated from bamboo leaves, all of which are also found in numerous plants. 2,3) It is reported that these flavonoids possess anxiolytic properties 4,5) and that Orientin possesses antioxidative activity, 6) however, there is no information about the vasorelaxant effect of these compounds. In the present investigation, we examined the vasodilatation effects of these flavonoid C-glycosyl compounds and found only Orientin produced a vasorelaxant effect on rabbit aortic rings. The data from the present study suggested that Orientin relaxed thoracic aortic rings by the nitric oxide-guanosine 3,5-cyclic monophosphate (cGMP) pathway, and in the vascular smooth muscle inhibited the contraction induced by the activation of receptor-operating and voltage-dependent Ca 2ϩ channels. Cyclooxygenase pathway, potassium channels, b-receptors and adenosine-3Ј,5Ј-cyclic phosphoric acid (cAMP) pathway, on the other hand, had no apparent roles. The inhibition of both intracellular Ca 2ϩ release and extracellular Ca 2ϩ influx may be one of the main vasorelaxant mechanisms of Orientin. MATERIALS AND METHODS Animal

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Endothelium-Dependent Vasorelaxation Induced by Black Currant Concentrate in Rat Thoracic Aorta

Cited by 73 publications

References 28 publications

Antioxidant and Vasodilatory Effects of Blackberry and Grape Wines

Antioxidant and Vasodilatory Effects of Blackberry and Grape Wines

Purified Anthocyanin Supplementation Improves Endothelial Function via NO-cGMP Activation in Hypercholesterolemic Individuals

Vasodilatation Produced by Orientin and Its Mechanism Study

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