Endothelium Independent Effect of Pelargonidin on Vasoconstriction in Rat Aorta

Abstract

In this study, we investigated the effects of pelargonidin, an anthocyanidin found in many fruits and vegetables, on endothelium-independent vascular contractility to determine the underlying mechanism of relaxation. Isometric contractions of denuded aortic muscles from male rats were recorded, and the data were combined with those obtained in western blot analysis. Pelargonidin significantly inhibited fluoride-, thromboxane A2-, and phorbol ester-induced vascular contractions, regardless of the presence or absence of endothelium, suggesting a direct effect of the compound on vascular smooth muscles via a different pathway. Pelargonidin significantly inhibited the fluoride-dependent increase in the level of myosin phosphatase target subunit 1 (MYPT1) phosphorylation at Thr-855 and the phorbol 12,13-dibutyrate-dependent increase in the level of extracellular signal-regulated kinase (ERK) 1/2 phosphorylation at Thr202/Tyr204, suggesting the inhibition of Rho-kinase and mitogen-activated protein kinase kinase (MEK) activities and subsequent phosphorylation of MYPT1 and ERK1/2. These results suggest that the relaxation effect of pelargonidin on agonist-dependent vascular contractions includes inhibition of Rho-kinase and MEK activities, independent of the endothelial function.

Polyphenols, Luteolin and Pelargonidin, Modulate Radio- and Chemo-Sensitivity on Breast Cancer

“…Pelargonidin (PEL) [2-(4-hydroxyphenyl) chromenylium-3,5,7-triol], an anthocyanidin, has been reported to possess a strong antioxidant effect via reactive oxygen species (ROS) scavenging ability and an anti-inflammatory role through its master inhibition of nuclear factor kappa B (NF-κB) activity (Hämäläinen et al, 2007). PEL has been investigated in numerous preclinical models, such as diabetes, cardiovascular disorders, and cancers (Noda et al, 2002;Min et al, 2018).…”

Flavonoids and cardiovascular risk factors: a review

“…Flavonoids relax a plethora of conduit and resistance arteries and veins (58). Mechanistically, this vasodilation can be mediated by: 1) blockade of Ca 2+ channels (65,66); 2) opening of K + channels (67,68); 3) inhibition of Ca 2+ release from (69) or stimulation of Ca 2+ uptake to intracellular stores (70); 4) inhibition of protein kinase C (71), Rho-kinase (72,73), and phosphodiesterases (74,75); 5) stimulation of soluble guanylyl cyclase (76), protein kinase A (76,77), and protein kinase G (78); and/or 6) stimulation of G-protein coupled receptors like β adreno-receptor (79). Notably, the same flavonoid can induce vasodilation through one or more of these pathways.…”

Pelargonidin as an Attenuator of Neuronal Mitochondrial Dysfunction: An in Vivo Study

“…In addition, PEL was investigated as an anti-in ammatory avonoid via inhibiting the production of nitric oxide (NO), inducible nitric the expression of oxide synthase (iNOS) and the activity of NF-κB (Hamalainen et al 2007). In vivo, PEL has been studied in a variety of experimental models; viz, atherosclerosis, diabetes and cardiovascular dysfunction (Noda et al 2002;Min et al 2018).…”