Endotoxin and macrophage-migration inhibition

Fox, Roy; Rajaraman, K.

doi:10.1016/0008-8749(80)90333-0

Cited by 34 publications

(8 citation statements)

References 23 publications

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“…[18][19][20][21][22] It has been demonstrated that endotoxin levels in the peritoneal fluid and blood are higher in E coli plus bile peritonitis than in E coli peritonitis shortly after induction of peritonitis.23 Because peritoneal macro¬ phages can appear in thoracic duct lymph, it is thus pos¬ sible that lymphatic transport of phagocytes with low numbers of ingested bacteria contributed to the finding of impaired peritoneal transport in £ coli plus bile peritonitis. However, it appears that variation in lymphatic absorp¬ tion of phagocytized bacteria should play a minor role for the differences in blood radioactivity during the early hours of peritonitis because of the slow transport of peri¬ toneal macrophages.24…”

Section: Injection Of Labeled E Colimentioning

confidence: 99%

Effect of Bile on Growth, Peritoneal Absorption, and Blood Clearance of Escherichia coli in E coli Peritonitis

Andersson

Schalén²,

Tranberg³

1991

Arch Surg

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The effect of intraperitoneal bile on growth, peritoneal absorption, and clearance of Escherichia coli was determined in E coli peritonitis in the rat. In E coli peritonitis, intraperitoneal bacterial counts gradually decreased, whereas they increased (after 2 hours) with subsequent development of bacteremia in E coli plus bile peritonitis. After an intraperitoneal injection of labeled bacteria, blood radioactivity was only initially lower in E coli plus bile peritonitis compared with E coli peritonitis. Clearance from blood was lower in E coli plus bile peritonitis than in E coli peritonitis. Organ localization was similar in E coli peritonitis and E coli plus bile peritonitis with decreased splenic, increased pulmonary, and unchanged hepatic uptakes compared with controls. Impaired peritoneal absorption of bacteria, together with impaired local host defense, is likely to enhance the noxious effect of bile in E coli peritonitis.

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Section: Injection Of Labeled E Colimentioning

confidence: 99%

Effect of Bile on Growth, Peritoneal Absorption, and Blood Clearance of Escherichia coli in E coli Peritonitis

Andersson

Schalén²,

Tranberg³

1991

Arch Surg

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“…The mean migration index for 3 mice immunized with complete adjuvant was 103.0 ±2.5; for 5 mice immunized with incomplete adjuvant was 93.8 ±8.2 and for 3 nonimmunized mice was 99.7 ± 4.4. Thus IL-1 did not signif icantly alter the migration areas of these cells in the absence of other stimuli, making it unlikely that there was sufficient contaminating endotoxin in the IL-1 preparation to affect the results [10],…”

Section: Resultsmentioning

confidence: 99%

Manipulation of Macrophage Migration Inhibition/Stimulation Responses by Adjuvants and Interleukins

MacSween¹,

Rajaraman²,

Rajaraman³

1990

Int Arch Allergy Immunol

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C57 B1/6 mice were immunized with bovine serum albumin (BSA) and Freund’s complete and incomplete adjuvants in various concentrations. Spleen cells from these animals were subsequently stimulated with concanavalin A (Con A), purified protein derivative or BSA, and lymphokine responses were measured in one-stage migration assays. Con A consistently produced macrophage migration inhibition factor (MIF) responses in nonimmunized animals and those immunized with complete adjuvant. This was switched to migration stimulation factor (MStF) responses by prior immunization with incomplete adjuvant. Immunization with complete adjuvant and with BSA alone was followed by MIF responses to antigenic stimulation whereas incomplete adjuvant promoted MStF responses. The MStF responses to both mitogenic and antigenic stimulation were inhibited by the addition of affinity purified interleukin-1 to the spleen cells. Interleukin-1 also inhibited MStF responses and potentiated MIF responses to Con A stimulation by human mononuclear cells whereas interleukin-2 did not influence these responses

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“…The frequency of the LPS-responding CFU, remained nearly constant even in the animals in which CSF-producing cells had been furnished with tolerance against LPS. Since it is known that the property to stop migration in response to LPS is rendered to mature macrophages (Fox and Rajaraman, 1980), it seems that the LPS-responding CFU, represent a fairly well-differentiated stage of the cells of monocytelmacrophage lineage. About one-third of CFU, produced colonies of the intermediate type, when the cultures were supplied with LPS.…”

Section: Discussionmentioning

confidence: 99%

Migration inhibitory action of bacterial lipopolysaccharide on progenitor cells of monocyte/macrophage lineage growing in culture in the presence of colony‐stimulating factor (CSF‐1)

Ishii

Shinoda

Shikita³

1982

Journal Cellular Physiology

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Addition of lipopolysaccharide (LPS) to the culture of mouse myeloid stem cells (CFUc) increased the incidence of compact colonies and decreased that of dispersed ones in the presence of colony-stimulating factor (CSF-1) which had not such an effect by itself even in high concentrations. Although colony morphology was thus changed, nearly all colonies were composed of monocytes. The incidence of compact colonies increased with the increase of LPS concentration but plateaued at about 50%. Bone marrow cells of LPS-tolerant mice responded to LPS in vitro to a slightly decreased extent. The activity of LPS was decreased by alkaline or acid hydrolysis of the LPS molecule and inhibited by polymixin B, but not by indomethacin, alpha-L-fucose, nor by alpha-methyl-D-mannoside. Other immunopotentiating substances, such as OK-432, Lentinan, and Levamisole, had no effect on the colony morphology. Both muramyl dipeptide and poly(I).poly(C) were also ineffective. Furthermore, the action of LPS was not abolished by the use of heat-inactivated serum in the culture. LPS was no longer stimulative for the induction of lysosomal enzymes in the CSF-stimulated culture, although it greatly enhanced the enzyme induction in the unstimulated culture. These results indicate that the cells of monocyte/macrophage lineage develop the capacity for migration before they become responsive to LPS, and that the LPS-responding monocytic cells can proliferate even in a state of confluence induced by LPS.

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Endotoxin and macrophage-migration inhibition

Cited by 34 publications

References 23 publications

Effect of Bile on Growth, Peritoneal Absorption, and Blood Clearance of Escherichia coli in E coli Peritonitis

Effect of Bile on Growth, Peritoneal Absorption, and Blood Clearance of Escherichia coli in E coli Peritonitis

Manipulation of Macrophage Migration Inhibition/Stimulation Responses by Adjuvants and Interleukins

Migration inhibitory action of bacterial lipopolysaccharide on progenitor cells of monocyte/macrophage lineage growing in culture in the presence of colony‐stimulating factor (CSF‐1)

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