Endotoxin Exacerbates Airway Inflammation but Attenuates Airway Hyperreactivity in Allergic Mice.

Jackson-Humbles, DN; Audo, AM; Buhs, RF; Birmingham, NP; Harkema, J.R.; Wagner, Johannes

doi:10.1164/ajrccm-conference.2009.179.1_meetingabstracts.a4257

Cited by 1 publication

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“…26,27 Lipopolysaccharide (LPS), a ligand for TLR4, has been reported to induce neutrophil recruitment in a similar animal model as used in the present study. 28 Airborne LPS commonly found in agricultural aerosols and PM is associated with increased airway inflammation in occupational lung disease. 29 BioPM from the four farms used in this study contain mainly ligands for TLR4 and the activation was blocked by using a TLR4 antagonist in monocytic cells.…”

Section: Discussionmentioning

confidence: 99%

Livestock farm particulate matter enhances airway inflammation in mice with or without allergic airway disease

Liu

Wagner

Harkema

et al. 2020

World Allergy Organization Journal

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Effects of airborne biological particulate matter (BioPM; from livestock farms) on the pulmonary airways are not well studied. The aim of the present study was to investigate whether fine (<2.5 mm) BioPM derived from indoor animal stables (two chicken and two pig farms) could modify airway allergic responses by using a mouse model of allergic airway disease (allergic asthma). After intraperitoneal ovalbumin (OVA) sensitization mice were either intranasally challenged with OVA (allergic mice) or saline (non-allergic controls). Mice were also intranasally treated with farmderived BioPM. Bronchoalveolar lavage fluid (BALF), blood and lung tissues were collected one day after intranasal exposure. BioPM from all the farms caused an acute neutrophilic inflammatory response in non-allergic mice. In allergic mice, BioPM derived from pig farm 2 induced a larger cellular inflammatory response than other farm-derived BioPM. All farm BioPM elicited Th17 cytokine (Interleukin (IL)-23) production except chicken farm 2, whereas Th2 cytokine (IL-5) increase was only induced by BioPM collected from chicken farm 2. These results indicate the exposure of BioPM from chicken and pig farms may cause the enhancement of airway allergic response in mice following exposure to OVA. More variation in the responses between farms was observed in allergic than non-allergic mice. Understanding the source and doses of BioPM that may affect the airway allergic response could help susceptible individuals to avoid worsening their respiratory diseases.

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