2019
DOI: 10.22207/jpam.13.1.10
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Endotoxin Production by Pseudomonas aeruginosa ATCC 9027 with Potential Medical Applications

Abstract: Four Gram-negative bacteria (E.coli AtCC 19404, P. aeruginosa AtCC9027, A. hydrophila AtCC 7966 and V. damsel ATCC 33539) were screened for production of endotoxin. P. aeruginosa recoded the highest endotoxin concentration (338.59 x 10 3 IU/ml). The optimization of endotoxin production resulted in the increase of endotoxin titer (25% at pH 7.0 and 26% at both 30°C and 90 rpm). The detection of lipid core complex (50 kDa) and O-specific polysccahrides (20, 25, 38 kDa) was performed by the application of SDS-PAG… Show more

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“…However, whereas there were only log 1.3 CFU/g content of ST NR in the ceca on D 21, the serum endotoxin levels were increased on D 21 due to ST NR infection. Other than Salmonella spp., diverse gram-negative bacteria such as Escherichia coli, Pseudomonas hydrophila, Aeromonas hydrophila , and Vibrio damsel produce endotoxins ( El-Naggar et al, 2019 ). Moreover, no differences were observed in the gut permeability on D 21 in this study.…”
Section: Discussionmentioning
confidence: 99%
“…However, whereas there were only log 1.3 CFU/g content of ST NR in the ceca on D 21, the serum endotoxin levels were increased on D 21 due to ST NR infection. Other than Salmonella spp., diverse gram-negative bacteria such as Escherichia coli, Pseudomonas hydrophila, Aeromonas hydrophila , and Vibrio damsel produce endotoxins ( El-Naggar et al, 2019 ). Moreover, no differences were observed in the gut permeability on D 21 in this study.…”
Section: Discussionmentioning
confidence: 99%