Endotoxin theory of atherosclerosis

Anikhovskaya, I. A.; Kubatiev, A. A.; Yakovlev, M. Yu.

doi:10.1134/s0362119715010028

Cited by 14 publications

(4 citation statements)

References 11 publications

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“…HUVECs have been used as in vitro models for the study of atherosclerosis (47). As an endotoxin, LPS is a potentially important stimulator and risk factor for atherosclerosis (48). LPS has been implicated in endothelial injury and has induced apoptosis and proliferation in endothelial and VSMCs, respectively (34,35,49).…”

Section: Discussionmentioning

confidence: 99%

CircRNA‑0044073 is upregulated in atherosclerosis and increases the proliferation and invasion of cells by targeting miR‑107

Shen

Lou

et al. 2019

Mol Med Report

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Circular RNAs (circRNAs) are endogenous non-coding RNAs implicated in atherosclerosis. The aim of the present study was to explore the function of circRNA-0044073 in atherosclerosis. Reverse transcription quantitative polymerase chain reaction assays were used to measure the expression levels of circRNA-0044073, microRNA (miRNA/miR)-107, janus kinase 1 (JAK1), signal transducer and activator of transcription 3 (STAT3), B-cell lymphoma 2 (Bcl-2) and v-myc avian myelocytomatosis viral oncogene homolog (c-myc) in in blood cells from patients with atherosclerosis. RNA pull-down and luciferase reporter assays were then used to determine the association between circRNA and miR expression, and miR and gene expression, respectively. Matrigel invasion assay and flow cytometry were used to analyze cell invasion and cell cycle. Western blot analysis and ELISA were used to evaluate the expression levels of proteins. It was identified that the expression of circRNA-0044073 was upregulated and the expression of miR-107 was downregulated in atherosclerotic blood cells. Overexpression of circRNA-0044073 promoted the proliferation of human vascular smooth muscle cells (HUVSMCs) and human vascular endothelial cells (HUVECs), while overexpression of miR-107 inhibited their proliferation. In addition, circRNA-0044073 suppressed the levels of miR-107 via a sponge mechanism. Lipopolysaccharide (LPS) affected the proliferation of HUVSMCs and HUVECs, and also resulted in changes in circRNA-0044073 expression levels. CircRNA-0044073 promoted the proliferation and invasion of HUVSMCs and HUVECs in spite of the opposite effect observed with LPS treatment. The JAK/STAT signaling pathway was activated in patients with atherosclerosis. CircRNA-0044073 favored the activation of the JAK/STAT signaling pathway and inflammation in HUVSMCs and HUVECs. These data indicate that circRNA-0044073 is upregulated in atherosclerosis and promotes the proliferation and invasion of cells by targeting miR-107 and activating the JAK/STAT signaling pathway, potentially offering a target for novel treatment strategies against atherosclerosis.

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Section: Discussionmentioning

confidence: 99%

CircRNA‑0044073 is upregulated in atherosclerosis and increases the proliferation and invasion of cells by targeting miR‑107

Shen

Lou

et al. 2019

Mol Med Report

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“…Гипотеза о способности кишечного ЭТ быть одной из возможных причин индукции воспаления, выдвинутая более трети века назад [22,23], потребовала создания новых методов исследования [24] и поиска средств снижения уровня ЭТ в крови [25], комбинация которых получила название «мультивекторная антиэндотоксиновая составляющая» (МАЭС) [26]. Она индивидуальна, состоит из энтеросорбентов, эубиотиков, желчегонных и иных лекарств и процедур, способна существенно повышать эффективность лечения многих заболеваний, таких как аллергозы, сахарный диабет, женское бесплодие, вирусные инфекции и др.…”

Section: обсуждение результатовunclassified

“…Она индивидуальна, состоит из энтеросорбентов, эубиотиков, желчегонных и иных лекарств и процедур, способна существенно повышать эффективность лечения многих заболеваний, таких как аллергозы, сахарный диабет, женское бесплодие, вирусные инфекции и др. [10,26], и, что наиболее интересно для психиатров, нервной анорексии [27].…”

Section: обсуждение результатовunclassified

Сопряженность Процессов Системного Воспаления И Системной Эндотоксинемии При Эндогенных Психозах

Зозуля¹,

Отман²,

Олейчик³

et al. 2020

СВПН

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Objective of the study: to analyze the conjugacy of processes of systemic inflammation and systemic endotoxinemia in relation to the clinical characteristics of patients with endogenous psychoses (EGP) before and after psychopharmacotherapy. Material and Methods: the study includes 25 patients with schizophrenia (F20.0 (0-1) according to ICD-10) and schizoaffective psychosis (F25.0), examined at admission to the hospital (in an acute psychotic state) and before discharge (after performed pharmacotherapy). The control group consists of 25 healthy persons.

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“…Reverse cholesterol transport plays a crucial role in exporting cholesterol from peripheral cells in the prevention and treatment of atherosclerosis. It has been postulated that LPS can induce atherosclerosis [ 21 ]. One study indicated that quercetin improved macrophage reverse cholesterol transport in apolipoprotein E-deficient mice in an atherosclerosis model fed a high-fat diet [ 22 ], while another study found the quercetin could induce apolipoprotein A1 synthesis in hepatocytes [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Quercetin Alleviates Inflammation and Energy Deficiency Induced by Lipopolysaccharide in Chicken Embryos

Guo

et al. 2023

Animals

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Energy deficiency causes multiple organ dysfunctions after LPS induction. Quercetin is a phenolic compound found in herbal medicines. However, the effects of quercetin in alleviating LPS-induced energy deficiency remain unclear. In the present study, an in vivo LPS-induced inflammation model was established in chicken embryos. Specific pathogen-free chicken embryos (n = 120) were allocated to control, PBS with or without ethanol, quercetin (10, 20, or 40 nmol, respectively), and LPS (125 ng/egg) with or without quercetin groups. Fifteen day old embryonated eggs were injected with the abovementioned solutions via the allantoic cavity. On embryonic day 19, the tissues of the embryos were collected for histopathological examination using frozen oil red O staining, RNA extraction, real-time quantitative polymerase chain reaction, and immunohistochemical investigations. The glycogen and lipid contents in the liver increased after LPS stimulation as compared with the PBS group, whereas quercetin decreased the accumulation as compared with the LPS group. The mRNA expressions of AMPKα1 and AMPKα2 in the duodena, ceca, and livers were upregulated after LPS induction as compared with the PBS group, while quercetin could downregulate these expressions as compared with the LPS group. The immunopositivity of AMPKα2 in the villus, crypt, lamina propria, tunica muscularis, and myenteric plexus in the duodena and in the cytoplasms of hepatocytes significantly increased after LPS induction when compared with the PBS group (p < 0.01), whereas the immunopositivity to AMPKα2 in the quercetin treatment group significantly decreased when compared with the LPS group (p < 0.01 or p < 0.05). The LPS-induced high expressions of transcription factor PPARα and glucose transporter (SGLT1) were blocked by quercetin in the duodena, ceca, and livers. Quercetin treatment improved the LPS-induced decrease in APOA4 in the duodena, ceca, and livers. The mRNA expression of PEPT1 in the duodena and ceca increased after LPS challenge, whereas quercetin could downregulate PEPT1 gene expression. These data demonstrate that quercetin improved the energy deficiency induced by LPS in chicken embryos. The LPS-induced inflammation model was established to avoid the effect of LPS exposure from the environment and intestinal flora. The results form the basis the administration of quercetin pretreatment (in ovo infection) to improve the energy state of chicken embryos and improve the inflammation response.

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Endotoxin theory of atherosclerosis

Cited by 14 publications

References 11 publications

CircRNA‑0044073 is upregulated in atherosclerosis and increases the proliferation and invasion of cells by targeting miR‑107

CircRNA‑0044073 is upregulated in atherosclerosis and increases the proliferation and invasion of cells by targeting miR‑107

Сопряженность Процессов Системного Воспаления И Системной Эндотоксинемии При Эндогенных Психозах

Quercetin Alleviates Inflammation and Energy Deficiency Induced by Lipopolysaccharide in Chicken Embryos

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