Energetic benefits and rapid cellobiose fermentation by Saccharomyces cerevisiae expressing cellobiose phosphorylase and mutant cellodextrin transporters

Ha, Suk Jin; Galazka, Jonathan M.; Oh, Eun‐Suok; Kordić, Vesna; Kim, Heejin; Jin, Yong‐Su; Cate, J.H.D.

doi:10.1016/j.ymben.2012.11.005

Cited by 62 publications

(90 citation statements)

References 39 publications

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“…The intracellular b-glucosidase can be replaced by cellobiose phosphorylase which produces glucose and glucose-1-phoshate from cellobiose. Efficient cellobiose fermentation by engineered yeast expressing a cellobiose transporter and a cellobiose phosphorylase has also been demonstrated ( Figure 1c) [38].…”

Section: Metabolic Engineering For the Utilization Of Non-glucose Sugarsmentioning

confidence: 97%

Combining C6 and C5 sugar metabolism for enhancing microbial bioconversion

Zhang

Liu

Kong

et al. 2015

Current Opinion in Chemical Biology

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Section: Metabolic Engineering For the Utilization Of Non-glucose Sugarsmentioning

confidence: 97%

Combining C6 and C5 sugar metabolism for enhancing microbial bioconversion

Zhang

Liu

Kong

et al. 2015

Current Opinion in Chemical Biology

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“…The first structural determination of the enzyme was reported in Cellvibrio gilvus (Hidaka et al 2006), which was the second structural determination in GH94 after that of N,N'-diacetylchitobiose phosphorylase (Hidaka et al 2004). Cellobiose phosphorylases are often used to increase the efficiency of the fermentation of cellulosic biomass by Saccharomyces caused by intracellular expression of the gene (Aeling et al 2012;Ha et al 2013;Chomvong et al 2014). These enzymes are also used in the production of amylose from cellulosic materials by the concerted action of glycogen phosphorylase (Ohdan et al 2007;You et al 2013).…”

Section: Phosphorylases In Gh94mentioning

confidence: 99%

Diversity of phosphorylases in glycoside hydrolase families

Kitaoka

2015

Appl Microbiol Biotechnol

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Phosphorylases are useful catalysts for the practical preparation of various sugars. The number of known specificities was 13 in 2002 and is now 30. The drastic increase in available genome sequences has facilitated the discovery of novel activities. Most of these novel phosphorylase activities have been identified through the investigations of glycoside hydrolase families containing known phosphorylases. Here, the diversity of phosphorylases in each family is described in detail.

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“…The recombinant strain was able to produce ethanol directly from cellooligosaccharide. Furthermore, addition of cellulase to the SSF process resulted in ethanol production from cellulose with more efficiency compared to the wild-type strain (Galazka et al 2010;Ha et al, 2013). Lee et al (2013) demonstrated efficient ethanol production without supplementation of β-glucosidase using an engineered S. cerevisiae strain expressing a cellodextrin transporter and an intracellular β-glucosidase from N. crassa.…”

Section: Cellodextrin Transportersmentioning

confidence: 99%

Advances in consolidated bioprocessing systems for bioethanol and butanol production from biomass: a comprehensive review

2015

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HIGHLIGHTS Various CBP strategies have been discussed. Recently, lignocellulosic biomass as the most abundant renewable resource has been widely considered for bioalcohols production. However, the complex structure of lignocelluloses requires a multi-step process which is costly and time consuming. Although, several bioprocessing approaches have been developed for pretreatment, saccharification and fermentation, bioalcohols production from lignocelluloses is still limited because of the economic infeasibility of these technologies. This cost constraint could be overcome by designing and constructing robust cellulolytic and bioalcohols producing microbes and by using them in a consolidated bioprocessing (CBP) system. This paper comprehensively reviews potentials, recent advances and challenges faced in CBP systems for efficient bioalcohols (ethanol and butanol) production from lignocellulosic and starchy biomass. The CBP strategies include using native single strains with cellulytic and alcohol production activities, microbial co-cultures containing both cellulytic and ethanologenic microorganisms, and genetic engineering of cellulytic microorganisms to be alcohol-producing or alcohol producing microorganisms to be cellulytic. Moreover, high-throughput techniques, such as metagenomics, metatranscriptomics, next generation sequencing and synthetic biology developed to explore novel microorganisms and powerful enzymes with high activity, thermostability and pH stability are also discussed. Currently, the CBP technology is in its infant stage, and ideal microorganisms and/or conditions at industrial scale are yet to be introduced. So, it is essential to bring into attention all barriers faced and take advantage of all the experiences gained to achieve a high-yield and low-cost CBP process.

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Energetic benefits and rapid cellobiose fermentation by Saccharomyces cerevisiae expressing cellobiose phosphorylase and mutant cellodextrin transporters

Cited by 62 publications

References 39 publications

Combining C6 and C5 sugar metabolism for enhancing microbial bioconversion

Combining C6 and C5 sugar metabolism for enhancing microbial bioconversion

Diversity of phosphorylases in glycoside hydrolase families

Advances in consolidated bioprocessing systems for bioethanol and butanol production from biomass: a comprehensive review

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