Energetics of alkalophilic representatives of the genus Bacillus

Muntyan, Maria S.; Popova, Inna E.; Bloch, Dmitry A.; Skripnikova, Elena; Ustiyan, V.S.

doi:10.1007/s10541-005-0092-5

Cited by 10 publications

(5 citation statements)

References 53 publications

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“…This situation may be similar to the state of bacteria with a decrease in energy resources, when cells became motile (Figure 3a). It is likely that, both in the presence of HQNO and with a deficit of energy resources, a decrease in Δψ serves as a However, such a flagellar motor could be started either directly by ∆s-generated by means of a primary Na + pump, as in Vibrio species at alkaline pH [49]-or indirectly, when ∆p, generated by a primary H + pump at neutral pH, is converted into ∆s by a secondary Na + -pumping mechanism-for example, a Na + /H + -antiporter [49]-as it also takes place in alkaliphilic Bacillus species (for review, see [50]). In order to find out what kind of energy transfer mechanism was used by the cells to initiate operation of the flagellar motor, we investigated the effects of protonophores and some inhibitors.…”

Section: Resultsmentioning

confidence: 99%

Sodium Energetic Cycle in the Natronophilic Bacterium Thioalkalivibrio versutus

Muntyan

Viryasov

Sorokin

et al. 2022

IJMS

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As inhabitants of soda lakes, Thioalkalivibrio versutus are halo- and alkaliphilic bacteria that have previously been shown to respire with the first demonstrated Na+-translocating cytochrome-c oxidase (CO). The enzyme generates a sodium-motive force (Δs) as high as −270 mV across the bacterial plasma membrane. However, in these bacteria, operation of the possible Δs consumers has not been proven. We obtained motile cells and used them to study the supposed Na+ energetic cycle in these bacteria. The resulting motility was activated in the presence of the protonophore 2-heptyl-4-hydroxyquinoline N-oxide (HQNO), in line with the same effect on cell respiration, and was fully blocked by amiloride—an inhibitor of Na+-motive flagella. In immotile starving bacteria, ascorbate triggered CO-mediated respiration and motility, both showing the same dependence on sodium concentration. We concluded that, in T. versutus, Na+-translocating CO and Na+-motive flagella operate in the Na+ energetic cycle mode. Our research may shed light on the energetic reason for how these bacteria are confined to a narrow chemocline zone and thrive in the extreme conditions of soda lakes.

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Section: Resultsmentioning

confidence: 99%

Sodium Energetic Cycle in the Natronophilic Bacterium Thioalkalivibrio versutus

Muntyan

Viryasov

Sorokin

et al. 2022

IJMS

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“…And in the end, the only isolates identified as Staphylococcus pasteuri , Sp-12 and isolates identified as Uncultured bacterium clone ELU0126-T312-S-NI-000133 were originated from the samples 3 and 5 or 2 and 3, respectively. Bacillus and Arthrobacter species have been found so far in extremely alkaline (pH up to 12.8) environments ( Kanekar et al., 2002 , Muntyan et al., 2005 ), in river sediments affected by the proximity of a petrochemical industrial site ( Narancic et al., 2012 ) and so on. Species of genus Kocuria might be isolated from various environments such as alkaline (pH 11.4) groundwater ( Tiago et al., 2004 ) and from different habitats in Antarctica ( Satyanarayana et al., 2005 ).…”

Section: Resultsmentioning

confidence: 99%

Alkaline Technosol contaminated by former mining activity and its culturable autochthonous microbiota

et al. 2017

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Technosols or technogenic substrates contaminated by potentially toxic elements as a result of iron mining causes not only contamination of the surrounding ecosystem but may also lead to changes of the extent, abundance, structure and activity of soil microbial community. Microbial biomass were significantly inhibited mainly by exceeding limits of potentially toxic metals as arsenic (in the range of 343–511 mg/kg), copper (in the range of 7980–9227 mg/kg), manganese (in the range of 2417–2670 mg/kg), alkaline and strong alkaline pH conditions and minimal contents of organic nutrients. All of the 14 bacterial isolates, belonged to 4 bacterial phyla, Actinobacteria, Firmicutes; β- and γ-Proteobacteria. Thirteen genera and 20 species of microscopic filamentous fungi were recovered. The most frequently found species belonged to genera Aspergillus (A. clavatus, A. niger, A. flavus, A. versicolor, Aspergillus sp.) with the dominating A. niger in all samples, and Penicillium (P. canescens, P. chrysogenum, P. spinulosum, Penicillium sp.). Fungal plant pathogens occurred in all surface samples. These included Bjerkandera adustata, Bionectria ochloleuca with anamorph state Clonostachys pseudochloleuca, Lewia infectoria, Phoma macrostoma and Rhizoctonia sp.

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“…As new structural data complement the work on these adaptations the insights will be much greater. Work has not yet been reported on equally interesting motifs that have been observed in respiratory chain complexes features [159, 226, 233]. Such features and motifs may facilitate specific partnering between respiratory chain complexes and alkaliphile ATP synthases either directly or in close proximity.…”

Section: Discussionmentioning

confidence: 99%

F1F0-ATP synthases of alkaliphilic bacteria: Lessons from their adaptations

Hicks

Fujisawa

et al. 2010

Biochimica et Biophysica Acta (BBA) - Bioenergetics

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This review focuses on the ATP synthases of alkaliphilic bacteria and, in particular, those that successfully overcome the bioenergetic challenges of achieving robust H+-coupled ATP synthesis at external pH values > 10. At such pH values the protonmotive force, which is posited to provide the energetic driving force for ATP synthesis, is too low to account for the ATP synthesis observed. The protonmotive force is lowered at very high pH by the need to maintain a cytoplasmic pH well below the pH outside, which results in an energetically adverse pH gradient. Several anticipated solutions to this bioenergetic conundrum have been ruled out. Although the transmembrane sodium motive force is high under alkaline conditions, respiratory alkaliphilic bacteria do not use Na+-instead of H+-coupled ATP synthases. Nor do they offset the adverse pH gradient with a compensatory increase in the transmembrane electrical potential component of the protonmotive force. Moreover, studies of ATP synthase rotors indicate that alkaliphiles cannot fully resolve the energetic problem by using an ATP synthase with a large number of c-subunits in the synthase rotor ring. Increased attention now focuses on delocalized gradients near the membrane surface and H+ transfers to ATP synthases via membrane-associated microcircuits between the H+ pumping complexes and synthases. Microcircuits likely depend upon proximity of pumps and synthases, specific membrane properties and specific adaptations of the participating enzyme complexes. ATP synthesis in alkaliphiles depends upon alkaliphile-specific adaptations of the ATP synthase and there is also evidence for alkaliphile-specific adaptations of respiratory chain components.

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Energetics of alkalophilic representatives of the genus Bacillus

Cited by 10 publications

References 53 publications

Sodium Energetic Cycle in the Natronophilic Bacterium Thioalkalivibrio versutus

Sodium Energetic Cycle in the Natronophilic Bacterium Thioalkalivibrio versutus

Alkaline Technosol contaminated by former mining activity and its culturable autochthonous microbiota

F1F0-ATP synthases of alkaliphilic bacteria: Lessons from their adaptations

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