Energetics of gating MscS by membrane tension in azolectin liposomes and giant spheroplasts

Shaikh, Shaista; Cox, Charles D.; Nomura, Takeshi; Martinac, Boris

doi:10.4161/chan.28366

Cited by 26 publications

(25 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our result indicates that both inward and outward bending result in a similar membrane tension, T, and areal change, ΔA, and thus a similar gating energy, Δ G = TΔA, with Δ G being the gating energy. 38 However, we showed that formation of outward curvature is not favorable for opening MscL. Only inward curvature can make a bigger effective pore for passing the ions through the channel, whereas outward curvature reduces the radius of the pore in the narrowest part of MscL (Fig.…”

Section: Resultsmentioning

confidence: 81%

The effect of local bending on gating of MscL using a representative volume element and finite element simulation

et al. 2014

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 81%

The effect of local bending on gating of MscL using a representative volume element and finite element simulation

et al. 2014

View full text Add to dashboard Cite

“…Note that the equilibration is about ten times slower in the DHB than quantified in excised patches32 and DIBs34. This difference can be rationalized by a number of differences between the assays, including the membrane geometry, the membrane area (~100,000 μ m 2 and ~6 μ m 2 for DHBs (Supplementary Table S1) and excised patches35, respectively) and the equilibration process (lipid insertion in the DHB; monolayer sliding in excised patches and DIBs).…”

Section: Resultsmentioning

confidence: 98%

Activation of the mechanosensitive ion channel MscL by mechanical stimulation of supported Droplet-Hydrogel bilayers

Rosholm

Baker

Ridone

et al. 2017

Sci Rep

Self Cite

View full text Add to dashboard Cite

The droplet on hydrogel bilayer (DHB) is a novel platform for investigating the function of ion channels. Advantages of this setup include tight control of all bilayer components, which is compelling for the investigation of mechanosensitive (MS) ion channels, since they are highly sensitive to their lipid environment. However, the activation of MS ion channels in planar supported lipid bilayers, such as the DHB, has not yet been established. Here we present the activation of the large conductance MS channel of E. coli, (MscL), in DHBs. By selectively stretching the droplet monolayer with nanolitre injections of buffer, we induced quantifiable DHB tension, which could be related to channel activity. The MscL activity response revealed that the droplet monolayer tension equilibrated over time, likely by insertion of lipid from solution. Our study thus establishes a method to controllably activate MS channels in DHBs and thereby advances studies of MS channels in this novel platform.

show abstract

“…The structure of TRAAK revealed that the binding of lipid acyl chain resulted in structural changes which regulated the channel [8] (Fig 2b). [35]. A study on MscL, has also observed that opening by lyso-PC differs from tension-induced gating [36], suggesting lyso-PC may interact with MscL.…”

Section: Enter the Lipidsmentioning

confidence: 97%

Spectator no more, the role of the membrane in regulating ion channel function

Pliotas

Naismith

2017

Current Opinion in Structural Biology

View full text Add to dashboard Cite

A pressure gradient across a curved lipid bilayer leads to a lateral force within the bilayer. Following ground breaking work on eukaryotic ion channels, it is now known that many proteins sense this change in the lateral tension and alter their functions in response. It has been proposed that responding to pressure differentials may be one of the oldest signaling mechanisms in biology. The most well characterized mechanosensing ion channels are the bacterial ones which open when the pressure differential hits a threshold. Recent studies on one of these channels, MscS, have developed a simple molecular model for how they sense and adapt to pressure.Biochemical and structural studies on mechanosensitive channels from eukaryotes have disclosed pressure sensing mechanisms. In this review, we highlight these findings and discuss the potential for a general model for pressure sensing.3

show abstract

Energetics of gating MscS by membrane tension in azolectin liposomes and giant spheroplasts

Cited by 26 publications

References 35 publications

The effect of local bending on gating of MscL using a representative volume element and finite element simulation

The effect of local bending on gating of MscL using a representative volume element and finite element simulation

Activation of the mechanosensitive ion channel MscL by mechanical stimulation of supported Droplet-Hydrogel bilayers

Spectator no more, the role of the membrane in regulating ion channel function

Contact Info

Product

Resources

About