Energy Conservation in <i>Bacillus megaterium</i>

Downs, Andrew J.; Jones, Colin W.

doi:10.1042/bst0020526

Cited by 12 publications

(3 citation statements)

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“…coli W was grown in carbon-limited continous culture using a 500 ml capacity chemostat fitted with a simple oxygen analyser [11][12][13]. The culture medium consisted of a minimal salts solution [14] supplemented with nitrilo-triacetic acid (0.1 g • litre -1) plus 7 mM glycerol.…”

Section: Methodsmentioning

confidence: 99%

The effect of temperature on the molar growth yield and maintenance requirement of Escherichia coli W during aerobic growth in continuous culture

Farmer

Jones

1976

FEBS Letters

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Section: Methodsmentioning

confidence: 99%

The effect of temperature on the molar growth yield and maintenance requirement of Escherichia coli W during aerobic growth in continuous culture

Farmer

Jones

1976

FEBS Letters

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“…To this end, we carried out a number of substrate-loading experiments of the type which [27] (A. J. Downs and C. W. Jones, unpublished data) clearly indicated that, as with E. coli [lo], the endogenous -P H f / O ratios of approximately 4 resulted from the activity of protontranslocating loops (energy-coupling sites) 1 and 2 and thus showed that NADH was the major oxidisable endogenous substrate in unstarved cells. The results which were obtained with more complex organisms of groups111 and IV are shown in Table 4.…”

Section: Respiratory Activity Relative Activities N a D P H + N A D +mentioning

confidence: 99%

Bacterial Respiration‐Linked Proton Translocation and Its Relationship to Respiratory‐Chain Composition

Jones

Brice

Downs

et al. 1975

European Journal of Biochemistry

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1.The relationship between respiratory chain composition and the efficiency of respiration-linked proton translocation was studied in nine bacterial species of widely differing taxonomic and ecological status.2. All the bacteria investigated contained respiratory chain dehydrogenases, ubiquinone and/or menaquinone, cytochrome b and cytochrome oxidase aa3 and/or 0. In addition, some of these organisms also contained pyridine nucleotide transhydrogenase and/or cytochrome c.3. + H+/O ratios of whole cell suspensions oxidising endogenous substrates were in the approximate range 4-8 .mol H+ translocated per g-atom oxygen consumed. It was concluded from the observed --f H+/O ratios of cells loaded with specific substrates that proton-translocating loops 1 and 2 were present in all of the organisms investigated, but that loops 0 and 3 were dependent upon the presence of pyridine nucleotide transhydrogenase and cytochrome c respectively.4. The wide range in energy conservation efficiency which was observed in these organisms is discussed in relation to their respiratory chain composition and natural habitat.Bacterial respiratory systems are very similar in many respects to those present in the mitochondria of higher organisms. Both are membrane-bound, can contain similar types of respiratory carriers and are capable of conserving energy in a form which subsequently can be used either for ATP synthesis or for driving a variety of energy-requiring processes There is some preliminary evidence which suggests that this efficiency may vary between different organisms and within the same organism during growth under different conditions [4,5,14,15]. Obviously, such variations might reflect the diversity of respiratory carrier composition (and hence molecular organisation) which exists in bacterial systems. In order to test this hypothesis we have investigated the respiratory systems of nine species of bacteria which were selected to cover a wide range of natural habitats and to represent a wide spectrum of respiratory chain composition. The results of these investigations confirm that the efficiency of oxidative phosphorylation varies between these different organisms and is related to differences in their respiratory chain composition. MATERIALS AND METHODS OrganismsThe following organisms were used during this study: Acinetobacter lwoffi 4B, Bacillus megaterium D440, Bacillus subtilis D473, Klebsiella pneumoniae NCTC 5055 (all of which were the gift of the Medical Research Council Microbial Systematics Unit, University of Leicester) ; Escherichia coli W and Pseudomonas ovalis Chester (which were kindly donated by Professor H. L. Kornberg, F.R.S.); Hydrogenomonas eutropha H16 (= Alcaligenes eutrophus H16; ATCC

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“…Each point shows measurements mado on samples of effluent culture collected 4-5 days after a decrease in the enternng concentration of sulphate: *, dry weight of oells; o, polyscharide; A, glycerol. Direct polarographic measurements of oxygen concentration in the air supply to the chemostat and in the effluent gas were used to calculate the oxygen growth yield (Downs & Jones, 1974); the gas-flow rates over the oxygen electrode were adjusted to about 150ml/min. Values presented are means±s.D., and the number of determinations is given in parentheses (n.t., not tested).…”

Section: Conditioniyfor Suiphatejlimitedgrowthmentioning

confidence: 99%

Effects of sulphate-limited growth in continuous culture on the electron-transport chain and energy conservation in Escherichia coli K12

Poole

Haddock

1975

Biochemical Journal

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Growth of Escherichia coli K12 in a chemostat was limited by sulphate concentrations lower than 300 muM. The synthesis of extracellular polysaccharide and a change in morphology accompanied sulphate-limited growth. Growth yields with respect to the amount of glycerol or oxygen consumed were sixfold and twofold lower respectively under these conditions than when growth was limited by glycerol. Sulphate-limited cells lacked the proton-translocating oxidoreduction segment of the electron-transport chain between NADH and the cytochromes, and particles prepared from these cells lacked the energy-dependent reduction of NAD+ by succinate, DL-alpha-glycerophosphate or D-lactate, suggesting the loss of site-I phosphorylation. Glycerol-limited cells contained cytochrome b556, b562 and o, ubiquinone and low concentrations of menaquinone. Sulphate limitation resulted in the additional synthesis of cytochromes d, a1, b558 and c550; the amount of ubiquinone was decreased and menaquinone was barely detectable. Non-haem iron and acid-labile sulphide concentrations were twofold lower in electron-transport particles prepared from sulphate-limited cells. Recovery of site-I phosphorylation could not be demonstrated after incubating sulphate-limited cells with or without glycerol, in either the absence or presence of added sulphate. The loss of site-I phosphorylation in sulphate-limited cells is discussed with reference to the accompanying alterations in cytochrome composition of such cells. Schemes are proposed for the functional organization of the respiratory chains of E. coli grown under conditions of glycerol or sulphate limitation.

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Energy Conservation in Bacillus megaterium

Cited by 12 publications

References 0 publications

The effect of temperature on the molar growth yield and maintenance requirement of Escherichia coli W during aerobic growth in continuous culture

The effect of temperature on the molar growth yield and maintenance requirement of Escherichia coli W during aerobic growth in continuous culture

Bacterial Respiration‐Linked Proton Translocation and Its Relationship to Respiratory‐Chain Composition

Effects of sulphate-limited growth in continuous culture on the electron-transport chain and energy conservation in Escherichia coli K12

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