Energy conversion coupled to cyanide-resistant respiration in the yeasts and

Veiga, Alexandra; Arrabaça, João Daniel; Sansonetty, Filipe; Ludovico, Paula; Côrte‐Real, Manuela; Loureiro-Dias, Maria C.

doi:10.1016/s1567-1356(02)00189-7

Cited by 38 publications

(36 citation statements)

References 36 publications

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“…In yeast strains that possess functional complex I, the induced AOX activity can support cell growth in the presence of cyt path inhibitors without any visible adverse effects (Veiga et al 2003a). Rotenone, the complex I inhibitor, strongly inhibited wheat seedling growth and rotenone plus AA completely inhibited it (Fig.…”

Section: Discussionmentioning

confidence: 95%

Expression profiles of respiratory components associated with mitochondrial biogenesis during germination and seedling growth under normal and restricted conditions in wheat

et al. 2008

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Germination of plant embryo is a dynamic phase-changing process that is driven by a rapid increase in mitochondrial respiration. We studied the development of respiratory electron transport pathways and the profiles of their transcript and protein components during this critical period using wheat embryos. Oxygen consumption through both the cytochrome and alternative pathways increased rapidly upon imbibition. Quantitative RT-PCR analysis using specific primers and western blot analysis using specific antibodies suggested that this respiratory burst was supported both by the stored mRNA and protein components and ones synthesized de novo at least in the cytochrome pathway. Dry embryos also contained transcript and protein of alternative oxidase (AOX), but their levels remained constant during the studied period. By contrast, the alternative pathway capacity showed a marked increase when the cytochrome pathway was inhibited by antimycin A and this increase was associated with increased levels of AOX transcript and protein. Our results suggest that mitochondrial biogenesis is accompanied by sequential and differential gene expression and protein accumulation, and that AOX allows the complex I to continue to conserve energy thus to support embryo germination and initial seedling growth in wheat when the cytochrome pathway is restricted.

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Section: Discussionmentioning

confidence: 95%

Expression profiles of respiratory components associated with mitochondrial biogenesis during germination and seedling growth under normal and restricted conditions in wheat

et al. 2008

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“…In plants, the mitochondrial membrane potential remains unchanged, as is expected because of the non-proton-pumping nature of the AOX (48). In contrast, in filamentous fungi and yeasts respiring simultaneously by complex IV and AOX, a rapid collapse of the mitochondrial membrane potential is observed (46,47,49,50). Interestingly, the AOX-dependent respiratory chain in the filamentous fungus P. anserina, in which the AOX is only induced by the specific deficiency of complex IV (51), as in some other fungi (e.g.…”

Section: Is the Plant Respiratory Chain Organized In A More Dynamic Mmentioning

confidence: 90%

“Respirasome”-like Supercomplexes in Green Leaf Mitochondria of Spinach

Krause

Reifschneider

Vocke

et al. 2004

Journal of Biological Chemistry

125

107

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Higher plant mitochondria have many unique features compared with their animal and fungal counterparts. This is to a large extent related to the close functional interdependence of mitochondria and chloroplasts, in which the two ATP-generating processes of oxidative phosphorylation and photosynthesis, respectively, take place. We show that digitonin treatment of mitochondria contaminated with chloroplasts from spinach (Spinacia oleracea) green leaves at two different buffer conditions, performed to solubilize oxidative phosphorylation supercomplexes, selectively extracts the mitochondrial membrane protein complexes and only low amounts of stroma thylakoid membrane proteins. By analysis of digitonin extracts from partially purified mitochondria of green leaves from spinach using blue and colorless native electrophoresis, we demonstrate for the first time that in green plant tissue a substantial proportion of the respiratory complex IV is assembled with complexes I and III into "respirasome"-like supercomplexes, previously observed in mammalian, fungal, and non-green plant mitochondria only. Thus, fundamental features of the supramolecular organization of the standard respiratory complexes I, III, and IV as a respirasome are conserved in all higher eukaryotes. Because the plant respiratory chain is highly branched possessing additional alternative enzymes, the functional implications of the occurrence of respiratory supercomplexes in plant mitochondria are discussed.

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“…complex I) and alternative oxidase. 201,202 When S. cerevisiae is grown under aerobic conditions, most of the oxygen is used by the classical respiratory chain. Because S. cerevisiae is a fermentative yeast, it does not require an alternative respiratory pathway for growth on sugars when the classical respiratory chain is blocked by inhibitors or by genetic alterations (i.e.…”

Section: Levels Of Oxygen and Classification Of Yeast Speciesmentioning

confidence: 99%

Role of the non‐respiratory pathways in the utilization of molecular oxygen by Saccharomyces cerevisiae

Rosenfeld

Beauvoit

2003

Yeast

133

110

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Saccharomyces cerevisiae is a facultative anaerobe devoid of mitochondrial alternative oxidase. In this yeast, the structure and biogenesis of the respiratory chain, on the one hand, and the functional interactions of oxidative phosphorylation with the cellular energetic metabolism, on the other, are well documented. However, to our knowledge, the molecular aspects and the physiological roles of the non-respiratory pathways that utilize molecular oxygen have not yet been reviewed. In this paper, we review the various non-respiratory pathways in a global context of utilization of molecular oxygen in S. cerevisiae. The roles of these pathways are examined as a function of environmental conditions, using either physiological, biochemical or molecular data. Special attention is paid to the characterization of the so-called 'cyanide-resistant respiration' that is induced by respiratory deficiency, catabolic repression and oxygen limitation during growth. Finally, several aspects of oxygen sensing are discussed.

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Energy conversion coupled to cyanide-resistant respiration in the yeasts and

Cited by 38 publications

References 36 publications

Expression profiles of respiratory components associated with mitochondrial biogenesis during germination and seedling growth under normal and restricted conditions in wheat

Expression profiles of respiratory components associated with mitochondrial biogenesis during germination and seedling growth under normal and restricted conditions in wheat

“Respirasome”-like Supercomplexes in Green Leaf Mitochondria of Spinach

Role of the non‐respiratory pathways in the utilization of molecular oxygen by Saccharomyces cerevisiae

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