The multi-source electromechanical coupling renders energy management of plug-in hybrid electric vehicles (PHEVs) highly nonlinear and complex. Furthermore, the complicated nonlinear management process highly depends on knowledge of driving conditions, and hinders the control strategies efficiently applied instantaneously, leading to massive challenges in energy saving improvement of PHEVs. To address these issues, a novel learning based model predictive control (LMPC) strategy is developed for a serial-parallel PHEV with the reinforced optimal control effect in real time application. Rather than employing the velocity-prediction based MPC methods favored in the literature, an original reference-tracking based MPC solution is proposed with strong instant application capacity. To guarantee the optimal control effect, an online learning process is implemented in MPC via the Gaussian process (GP) model to address the uncertainties during state estimation. The tracking reference in LMPC based control problem in PHEV is achieved by a microscopic traffic flow analysis (MTFA) method. The simulation results validate that the proposed method can optimally manage energy flow within vehicle power sources in real time, highlighting its anticipated preferable performance.Index Terms-Optimal control strategy, leaning based model predictive control (LMPC), Gaussian process (GP) model, microscopic traffic flow analysis (MTFA), plug-in hybrid electric vehicle (PHEV).