Energy metabolism and metabolomics response of Pacific white shrimp Litopenaeus vannamei to sulfide toxicity

Li, Tongyu; Li, Erchao; Suo, Yantong; Xu, Zhixin; Jia, Yongyi; Qin, Jianguang; Chen, Liqiao; Gu, Zhenxin

doi:10.1016/j.aquatox.2016.12.010

Cited by 74 publications

(22 citation statements)

References 30 publications

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“…The supernatant was then transferred to a 2-ml GC/MS glass vial, and 15 μL from each sample were analyzed for quality control purposes. The hepatopancreas extracts were processed using a method described in a recent study (Li et al, 2017 ).…”

Section: Methodsmentioning

confidence: 99%

“…Recently, metabolomics studies have been widely applied in aquatic animals to elucidate the biological effects of hypoxic stressors on organisms (Hines et al, 2007 ; Hallman et al, 2008 ; Tuffnail et al, 2009 ; Lardon et al, 2013a , b ). Several analytical techniques have been well established and are frequently applied in metabolomics studies (Luo et al, 2007 ; Nudi et al, 2008 ; Shao et al, 2015 ; Li et al, 2017 ), such as liquid chromatography–mass spectrometry (LC–MS), nuclear magnetic resonance (NMR), high-performance liquid chromatography (HPLC), and gas chromatography–mass spectrometry (GC–MS). Among these analytical techniques, GC-MS is frequently used in metabolomics studies and is highly sensitive and reproducible (Ruan et al, 2013 ; Ren et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

“…Among these analytical techniques, GC-MS is frequently used in metabolomics studies and is highly sensitive and reproducible (Ruan et al, 2013 ; Ren et al, 2015 ). Importantly, GC-MS-based metabolomics analysis has been used to investigate the biological effects of sulfide pollution in shrimp (Li et al, 2017 ). Their work confirmed the applicability of GC-MS-based metabolomics analysis to characterize the biological effects of environmental stressors in crustaceans.…”

Section: Introductionmentioning

confidence: 99%

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Based on the Metabolomic Approach the Energy Metabolism Responses of Oriental River Prawn Macrobrachium nipponense Hepatopancreas to Acute Hypoxia and Reoxygenation

Sun

Guo

et al. 2018

Front. Physiol.

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Hypoxia represents a major physiological challenge for prawns and is a problem in aquaculture. Therefore, an understanding of the metabolic response mechanism of economically important prawn species to hypoxia and re-oxygenation is essential. However, little is known about the intrinsic mechanisms by which the oriental river prawn Macrobrachium nipponense copes with hypoxia at the metabolic level. In this study, we conducted gas chromatography-mass spectrometry-based metabolomics studies and assays of energy metabolism-related parameters to investigate the metabolic mechanisms in the hepatopancreas of M. nipponense in response to 2.0 O2/L hypoxia for 6 and 24 h, and reoxygenation for 6 h following hypoxia for 24 h. Prawns under hypoxic stress displayed higher glycolysis-related enzyme activities and lower mRNA expression levels of aerobic respiratory enzymes than those in the normoxic control group, and those parameters returned to control levels in the reoxygenated group. Our results showed that hypoxia induced significant metabolomic alterations in the prawn hepatopancreas within 24 h. The main metabolic alterations were depletion of amino acids and 2-hydroxybutanoic acid and accumulation of lactate. Further, the findings indicated that hypoxia disturbed energy metabolism and induced antioxidant defense regulation in prawns. Surprisingly, recovery from hypoxia (i.e., reoxygenation) significantly affected 25 metabolites. Some amino acids (valine, leucine, isoleucine, lysine, glutamate, and methionine) were markedly decreased compared to the control group, suggesting that increased degradation of amino acids occurred to provide energy in prawns at reoxygenation conditions. This study describes the acute metabolomic alterations that occur in prawns in response to hypoxia and demonstrates the potential of the altered metabolites as biomarkers of hypoxia.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Based on the Metabolomic Approach the Energy Metabolism Responses of Oriental River Prawn Macrobrachium nipponense Hepatopancreas to Acute Hypoxia and Reoxygenation

Sun

Guo

et al. 2018

Front. Physiol.

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“…It cannot be discounted that environmental stress factors contributed to the mortalities that accumulated gradually in the control shrimp. As water quality was not measured chemically, it is possible, for example, that the external filtration systems used unknowingly accumulated organic matter to become anaerobic and release low levels of hydrogen sulphide or ammonium (Bagarinao, 1992; Li et al., 2017; Liang et al., 2016). Other possibilities include handling stress associated with capture, transfer into a new rearing environment, weighing and injection as well as the shrimp regularly being disturbed to syphon out faeces and uneaten feed, partly exchange water and rake the sand gently each morning and afternoon to accurately count survivors.…”

Section: Discussionmentioning

confidence: 99%

Mourilyan virus pathogenicity in kuruma shrimp (Penaeus japonicus)

Cowley

2020

Journal of Fish Diseases

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The bunyavirus Mourilyan virus (MoV) occurs commonly in Black tiger (Penaeus monodon) and kuruma shrimp (Penaeus japonicus) farmed in eastern Australia. There is circumstantial evidence of MoV causing mortalities among P. japonicus moved from farm ponds to tanks for rearing as broodstock. To directly assess its pathogenic potential, independent cohorts of pond‐ (n = 24) or tank‐reared juvenile (n = 21) P. japonicus were challenged intramuscularly with a cephalothorax tissue homogenate of P. monodon containing high loads of MoV (1.48 ± 0.28 × 108 MoV RNA copies/µg total RNA). In each trial, mortalities accumulated gradually among the saline‐injected controls. Mortality onset occurred 12–14 days earlier in the pond‐reared shrimp, possibly due to them possessing low‐level pre‐existing MoV infections. Despite the time to onset of mortality differing, Kaplan–Meier survival analyses confirmed mortality rates to be significantly higher in both the pond‐ (p = .017) and tank‐reared shrimp (p = .031) challenged with MoV. RT‐qPCR data on shrimp sampled progressively over each trial showed high loads of MoV to establish following challenge and discounted GAV and other endemic viruses from contributing to mortality. Together, the data show that acute MoV infection can adversely compromise the survival of juvenile P. japonicus.

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“…The increase in carbohydrate metabolism may be one of the reasons for the increase in ETS activity. Energy metabolism plays an important role in the process of stress adaptation in aquatic animals (Li et al., ; Sánchez‐Paz, García‐Carreño, Hernández‐López, Muhlia‐Almazán, & Yepiz‐Plascencia, ), so the two crude extracts may prime shrimp to better respond to pathogen infection or environmental stress.…”

Section: Discussionmentioning

confidence: 99%

Effects of crude extracellular protein and crude intracellular polysaccharides ofVibrio alginolyticuson the growth, energy metabolism regulation and WSSV resistance ofLitopenaeus vannamei

Shan

Liu

et al. 2018

Aquacult Nutr

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This study aimed to investigate the effects of adding crude extracts of the extracellular protein (Ex‐Pro) and intracellular polysaccharides (In‐Poly) of Vibrio alginolyticus to diets, at a dosage of 10 g/kg, on the growth, energy metabolism and WSSV (White Spot Syndrome Virus) resistance of Litopenaeus vannamei (initial weight, 0.88 ± 0.04 g/shrimp). Growth and survival rate were not significantly affected by the crude extracts (p > 0.05). Shrimp fed Ex‐Pro crude extracts showed higher succinate dehydrogenase (SDH) activity in the hepatopancreas and muscle but lower lactate dehydrogenase (LDH) activity in the muscle (p < 0.05). The activities of hexokinase (HK), pyruvate kinase (PK), LDH and SDH in the hepatopancreas and the activity of SDH in the muscle were significantly increased by feeding In‐Poly crude extracts (p < 0.05). In contrast, the content of fatty acid synthase (FAS) in the muscle was significantly reduced by the crude extracts (p < 0.05). The contents of glucose and triglyceride and the activity of the electron transport system in the hepatopancreas were significantly increased by the crude extracts (p < 0.05), and the WSSV resistance of the shrimp was increased. These results indicated that the Ex‐Pro and In‐Poly crude extracts of V. alginolyticus could affect energy metabolism, and there was a correlation between WSSV resistance and energy metabolism in L. vannamei.

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Energy metabolism and metabolomics response of Pacific white shrimp Litopenaeus vannamei to sulfide toxicity

Cited by 74 publications

References 30 publications

Based on the Metabolomic Approach the Energy Metabolism Responses of Oriental River Prawn Macrobrachium nipponense Hepatopancreas to Acute Hypoxia and Reoxygenation

Based on the Metabolomic Approach the Energy Metabolism Responses of Oriental River Prawn Macrobrachium nipponense Hepatopancreas to Acute Hypoxia and Reoxygenation

Mourilyan virus pathogenicity in kuruma shrimp (Penaeus japonicus)

Effects of crude extracellular protein and crude intracellular polysaccharides ofVibrio alginolyticuson the growth, energy metabolism regulation and WSSV resistance ofLitopenaeus vannamei

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