Energy metabolism measured by 31P magnetic resonance spectroscopy in the healthy human brain

Rietzler, Andreas; Steiger, Ruth; Mangesius, Stephanie; Walchhofer, Lisa-Maria; Gothe, Raffaella Matteucci; Schocke, Michael; Gizewski, Elke R.; Grams, Astrid

doi:10.1016/j.neurad.2021.11.006

Cited by 22 publications

(40 citation statements)

References 45 publications

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“…This could be explained by a difference in metabolic flux between awake and anesthetized animals. Assessment of the PCr/ATP ratio in awake and anesthetized mice demonstrated that anesthetized mice had a decreased energy reserve compared to awake mice [ 37 , 38 ]. The Pi/ATP ratio reveals information about the rate of ATP hydrolysis.…”

Section: Discussionmentioning

confidence: 99%

A Mouse Holder for Awake Functional Imaging in Unanesthetized Mice: Applications in 31P Spectroscopy, Manganese-Enhanced Magnetic Resonance Imaging Studies, and Resting-State Functional Magnetic Resonance Imaging

et al. 2022

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Anesthesia is often used in preclinical imaging studies that incorporate mouse or rat models. However, multiple reports indicate that anesthesia has significant physiological impacts. Thus, there has been great interest in performing imaging studies in awake, unanesthetized animals to obtain accurate results without the confounding physiological effects of anesthesia. Here, we describe a newly designed mouse holder that is interfaceable with existing MRI systems and enables awake in vivo mouse imaging. This holder significantly reduces head movement of the awake animal compared to previously designed holders and allows for the acquisition of improved anatomical images. In addition to applications in anatomical T2-weighted magnetic resonance imaging (MRI), we also describe applications in acquiring 31P spectra, manganese-enhanced magnetic resonance imaging (MEMRI) transport rates and resting-state functional magnetic resonance imaging (rs-fMRI) in awake animals and describe a successful conditioning paradigm for awake imaging. These data demonstrate significant differences in 31P spectra, MEMRI transport rates, and rs-fMRI connectivity between anesthetized and awake animals, emphasizing the importance of performing functional studies in unanesthetized animals. Furthermore, these studies demonstrate that the mouse holder presented here is easy to construct and use, compatible with standard Bruker systems for mouse imaging, and provides rigorous results in awake mice.

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Section: Discussionmentioning

confidence: 99%

A Mouse Holder for Awake Functional Imaging in Unanesthetized Mice: Applications in 31P Spectroscopy, Manganese-Enhanced Magnetic Resonance Imaging Studies, and Resting-State Functional Magnetic Resonance Imaging

et al. 2022

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“…Boundary regions, as well as regions filled with air or bone are spared, to avoid voxel contamination. The measurements are acquired using the parameters given in Table 2, and based on a conventional sequence by Siemens and described by Hattingen et al, in order to ensure reproducibility and applicability for potential future clinical use [32][33][34].…”

Section: Sequence Planning-31p-mrsmentioning

confidence: 99%

“…Therefore, these parameters also have to be taken into account when investigating cerebral energy metabolism under pathological conditions. This renders the interpretation of 31P-MRS even more challenging [33].…”

Section: P-mrsmentioning

confidence: 99%

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Magnetic Resonance Spectroscopy in Diagnosis and Follow-Up of Gliomas: State-of-the-Art

Galijašević

Steiger

Mangesius

et al. 2022

Cancers

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Preoperative grade prediction is important in diagnostics of glioma. Even more important can be follow-up after chemotherapy and radiotherapy of high grade gliomas. In this review we provide an overview of MR-spectroscopy (MRS), technical aspects, and different clinical scenarios in the diagnostics and follow-up of gliomas in pediatric and adult populations. Furthermore, we provide a recap of the current research utility and possible future strategies regarding proton- and phosphorous-MRS in glioma research.

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“…31 P magnetic resonance spectroscopy (MRS) has been widely used to measure energy metabolism in the human brain and muscle (1; 2). The knowledge of T 1 and T 2 relaxation times of metabolites is essential to optimize the measurement parameters of 31 P MRS acquisition and quantification of metabolite concentration.…”

Section: Introductionmentioning

confidence: 99%

Time-efficient relaxation measurements by ³¹P MR fingerprinting in human brain at 7T

Lim

Widmaier

Wenz

et al. 2022

Preprint

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Purpose: The goal of the study is to develop 31P spectroscopic MR fingerprinting (MRF) at 7T to measure T1 and T2 relaxation times simultaneously and to compare time efficiency and test-retest reproducibility of MRF with conventional inversion recovery and multi-TE methods. Methods: A 31P MRF scheme was designed based on a balanced steady-state free precession type sequence. Dictionary was generated using the Bloch equations. B0 map was acquired experimentally and incorporated into the dictionary. Simulations were performed to evaluate estimation bias. 7 phantoms with different T1 and T2 relaxation times were prepared for MRF validation. 7 volunteers were scanned twice using both MRF and the conventional methods to evaluate the reproducibility. Results: In phantom measurements, T1 and T2 values between MRF and conventional methods demonstrated a good agreement with Pearson's correlation coefficients of 0.99 and 0.97, respectively. In in vivo experiments, estimated T1 by MRF were in good agreement with those measured by the inversion recovery and in literatures. On the other hand, estimated T2 values by MRF were shorter than those measured by the multi-TE method. 31P MRF method can reduce the acquisition time by 15 min providing less than 10% of mean CV for T1 estimations and less than 20% of mean CV for T2 estimations of metabolites. Conclusion: Our results shows the feasibility of simultaneous T1 and T2 measurements of 31P metabolites in human brain using the MRF technique at 7T. High reproducibility can be achieved especially for T1 measurement with 40% time reduction over conventional methods.

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Energy metabolism measured by 31P magnetic resonance spectroscopy in the healthy human brain

Cited by 22 publications

References 45 publications

A Mouse Holder for Awake Functional Imaging in Unanesthetized Mice: Applications in 31P Spectroscopy, Manganese-Enhanced Magnetic Resonance Imaging Studies, and Resting-State Functional Magnetic Resonance Imaging

A Mouse Holder for Awake Functional Imaging in Unanesthetized Mice: Applications in 31P Spectroscopy, Manganese-Enhanced Magnetic Resonance Imaging Studies, and Resting-State Functional Magnetic Resonance Imaging

Magnetic Resonance Spectroscopy in Diagnosis and Follow-Up of Gliomas: State-of-the-Art

Time-efficient relaxation measurements by ³¹P MR fingerprinting in human brain at 7T

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Energy metabolism measured by 31P magnetic resonance spectroscopy in the healthy human brain

Cited by 22 publications

References 45 publications

A Mouse Holder for Awake Functional Imaging in Unanesthetized Mice: Applications in 31P Spectroscopy, Manganese-Enhanced Magnetic Resonance Imaging Studies, and Resting-State Functional Magnetic Resonance Imaging

A Mouse Holder for Awake Functional Imaging in Unanesthetized Mice: Applications in 31P Spectroscopy, Manganese-Enhanced Magnetic Resonance Imaging Studies, and Resting-State Functional Magnetic Resonance Imaging

Magnetic Resonance Spectroscopy in Diagnosis and Follow-Up of Gliomas: State-of-the-Art

Time-efficient relaxation measurements by 31P MR fingerprinting in human brain at 7T

Contact Info

Product

Resources

About

Time-efficient relaxation measurements by ³¹P MR fingerprinting in human brain at 7T