Energy metabolism of nerve cells during differentiation

Dittmann, L.; Hertz, Leif; Schousboe, Arne; Fosmark, Hanne; Sensenbrenner, M.; Mandel, P.

doi:10.1016/0014-4827(73)90315-7

Cited by 23 publications

(6 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The early and sustained rise in the respiratory rate of maturing osteoblasts is likely to reflect the high metabolic demands placed on cells when they are dividing and differentiating and is consistent with the finding that V O 2 is high in intact bone tissue (30). Similarly, oxidative phosphorylation increases during differentiation of other cell types in vitro, including, for example, placental trophoblasts (3), nerve cells (6), and colon adenocarcinoma cells (10).…”

Section: Discussionsupporting

confidence: 68%

Bioenergetics and mitochondrial transmembrane potential during differentiation of cultured osteoblasts

Komarova

Ataullakhanov

Globus

2000

American Journal of Physiology-Cell Physiology

175

133

View full text Add to dashboard Cite

To evaluate the relationship between osteoblast differentiation and bioenergetics, cultured primary osteoblasts from fetal rat calvaria were grown in medium supplemented with ascorbate to induce differentiation. Before ascorbate treatment, the rate of glucose consumption was 320 nmol. h(-1). 10(6) cells(-1), respiration was 40 nmol. h(-1). 10(6) cells(-1), and the ratio of lactate production to glucose consumption was approximately 2, indicating that glycolysis was the main energy source for immature osteoblasts. Ascorbate treatment for 14 days led to a fourfold increase in respiration, a threefold increase in ATP production, and a fivefold increase in ATP content compared with that shown in immature cells. Confocal imaging of mitochondria stained with a transmembrane potential-sensitive vital dye showed that mature cells possessed abundant amounts of high-transmembrane-potential mitochondria, which were concentrated near the culture medium-facing surface. Acute treatment of mature osteoblasts with metabolic inhibitors showed that the rate of glycolysis rose to maintain the cellular energy supply constant. Thus progressive differentiation coincided with changes in cellular metabolism and mitochondrial activity, which are likely to play key roles in osteoblast function.

show abstract

Section: Discussionsupporting

confidence: 68%

Bioenergetics and mitochondrial transmembrane potential during differentiation of cultured osteoblasts

Komarova

Ataullakhanov

Globus

2000

American Journal of Physiology-Cell Physiology

175

133

View full text Add to dashboard Cite

show abstract

“…l), it is reasonable to suggest that as the neurons differentiate their rate of oxygen consumption increases. A similar metabolic increase with differentiation has been observed by Dittmann et al (19) and Nissen et al (21,22) in their cultures of cells from 7-day chick embryo brains and from neuroblastomas. However, Romanoff (2) found no differences in the oxygen consumption by chick embryo brains between the 4th and the 21st day of incubation.…”

supporting

confidence: 64%

“…The anterior motor horn neurons in older embryos develop from the cells of the stage 17 basal plates. As early as the second day (stages [13][14][15][16][17][18][19] of incubation, the fate of the cells in the spinal cord becomes fixed. Thus, in the present study the tissue cube excised from stage 11 embryos is the direct developmental progenator of the stage 36 and 42 spinal cord motor horns.…”

mentioning

confidence: 99%

Rates of Oxygen Uptake by Embryonic Anterior Horn Tissue Isolated at Various Developmental Stages

Fisher

Hertz

1975

Experimental Biology and Medicine

Self Cite

View full text Add to dashboard Cite

Little information is available on the oxygen consumption of specific portions of the central nervous system during their initial stages of embryonic development (1, 2). The spinal cord motor horns of chick embryos are well suited for such a study since their development has been well described (3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15). Therefore, in order to follow changes in the respiration rate of a neural tissue as it develops from neuroepithelium into arrays of neurons we measured the oxygen consumption of fragments of the anterior motor horns of chick embryo spinal cords at various stages of development.Methods. Fertile white Leghorn eggs were obtained from the Poultry Science Department, University of Saskatchewan, and were incubated at 38" until the desired embryonic stages (16) were reached, i.e., stages 11 k 1, 17, 26 =t 1, 36, and 42 (approximately 13i, 255, 5 , 10, and 16 days of incubation, respectively). The embryos were removed from the eggs, placed in Hanks' balanced salt solution and carefully staged. Under a dissecting microscope the spinal cord, brachial or presumptive brachial level, was dissected out of the embryo and transferred to incubation medium (150 m M NaC1; 3.0 m M KCl; 1.7 m M KH2P04; 8.0 m M Na2HP0,.7H20; 1.0 m M CaC12 .2H20; 0.6 m M MgCl,; 7 m M D-glucose; pH = 7.4, 327 mOsm per kg water) lying over 2 % "Special Agar-Noble" (Difco). Small cubes averaging approximately 10 X lo5 pm3 in size (range 5-30 X lo5 pm3) were cut out of the motor horns, or presumptive motor horns (see Fig. 1) by pressing finely sharpened tungsten needles through the cord into the agar. The actual dimensions of each cube were measured by means of a This work was supported by grants from the Medical Research Council of Canada (M.R.C.) to Drs.

show abstract

“…The cultured neurons exhibited a high rate of oxygen consumption as well as a high ATP content. Neuronal exposure to a high potassium concentration had no effect on the cellular content of ATP, whereas exposure of astrocytes to high potassium led to a dramatic decrease in the ATP content (Dittmann, Hertz, et al, 1973; Schousboe et al, 1970). Notably, astrocytes and neurons had similar respiration rates on a per‐cell basis (Dittmann, Sensenbrenner, et al, 1973) and the rate of astrocytic oxygen consumption (CMR O2 ) was stimulated by 55 mM K + (Hertz et al, 1973; Hertz & Hertz, 1979).…”

Section: Preparation and Properties Of Astrocyte And Neuron Culturesmentioning

confidence: 99%

A tribute to Leif Hertz: The historical context of his pioneering studies of the roles of astrocytes in brain energy metabolism, neurotransmission, cognitive functions, and pharmacology identifies important, unresolved topics for future studies

Dienel

Schousboe

McKenna

et al. 2023

Journal of Neurochemistry

View full text Add to dashboard Cite

Leif Hertz, M.D., D.Sc. (honōris causā) (1930–2018), was one of the original and noteworthy participants in the International Conference on Brain Energy Metabolism (ICBEM) series since its inception in 1993. The biennial ICBEM conferences are organized by neuroscientists interested in energetics and metabolism underlying neural functions; they have had a high impact on conceptual and experimental advances in these fields and on promoting collaborative interactions among neuroscientists. Leif made major contributions to ICBEM discussions and understanding of metabolic and signaling characteristics of astrocytes and their roles in brain function. His studies ranged from uptake of K+ from extracellular fluid and its stimulation of astrocytic respiration, identification, and regulation of enzymes specifically or preferentially expressed in astrocytes in the glutamate–glutamine cycle of excitatory neurotransmission, a requirement for astrocytic glycogenolysis for fueling K+ uptake, involvement of glycogen in memory consolidation in the chick, and pharmacology of astrocytes. This tribute to Leif Hertz highlights his major discoveries, the high impact of his work on astrocyte–neuron interactions, and his unparalleled influence on understanding the cellular basis of brain energy metabolism. His work over six decades has helped integrate the roles of astrocytes into neurotransmission where oxidative and glycogenolytic metabolism during neurotransmitter glutamate turnover are key aspects of astrocytic energetics. Leif recognized that brain astrocytic metabolism is greatly underestimated unless the volume fraction of astrocytes is taken into account. Adjustment for pathway rates expressed per gram tissue for volume fraction indicates that astrocytes have much higher oxidative rates than neurons and astrocytic glycogen concentrations and glycogenolytic rates during sensory stimulation in vivo are similar to those in resting and exercising muscle, respectively. These novel insights are typical of Leif's astute contributions to the energy metabolism field, and his publications have identified unresolved topics that provide the neuroscience community with challenges and opportunities for future research.image

show abstract

Energy metabolism of nerve cells during differentiation

Cited by 23 publications

References 21 publications

Bioenergetics and mitochondrial transmembrane potential during differentiation of cultured osteoblasts

Bioenergetics and mitochondrial transmembrane potential during differentiation of cultured osteoblasts

Rates of Oxygen Uptake by Embryonic Anterior Horn Tissue Isolated at Various Developmental Stages

A tribute to Leif Hertz: The historical context of his pioneering studies of the roles of astrocytes in brain energy metabolism, neurotransmission, cognitive functions, and pharmacology identifies important, unresolved topics for future studies

Contact Info

Product

Resources

About