Energy related germination and survival rates of water-imbibed Arabidopsis seeds irradiated with protons

Qin, Huaili; Xue, Jianming; Lai, Jiangnan; Wang, J.Y.; Zhang, W.M.; Qi, Miao; Yan, Sha; Zhao, Wenbo; He, Fei; Gu, Hongya; Wang, Y.G.

doi:10.1016/j.nimb.2005.11.136

Cited by 6 publications

(3 citation statements)

References 9 publications

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“…Each point is the average of two independent experiments and the error bar is standard deviation. (Qin et al 2006). Thus we propose that the SAM is not the only radiation target in the seed; the tissue outside SAM could also serve as a radiobiological target, which we named the secondary target.…”

Section: Germination and Survival Versus Irradiation Energymentioning

confidence: 99%

Biological effects of protons targeted to different ranges in Arabidopsis seeds

Qin

Wang

Xue

et al. 2007

International Journal of Radiation Biology

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Section: Germination and Survival Versus Irradiation Energymentioning

confidence: 99%

Biological effects of protons targeted to different ranges in Arabidopsis seeds

Qin

Wang

Xue

et al. 2007

International Journal of Radiation Biology

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“…The LET of 1.89 MeV protons is higher than those of gamma rays and electrons, and thus, low-energy protons can induce more mutations than other low-LET ionizing radiations. Low-energy proton ion beams have previously been used to irradiate seeds of the model plant Arabidopsis thaliana, and it has been determined that 2.6 MeV ion beams are more effective than 6.5 MeV beams and that water-soaked seeds are more sensitive to proton ion beams than are dry seeds (Qin et al, 2006;Qin et al, 2007). These results suggest that ion beams absorbed by a target cell can cause more damage than ion beams that only pass through the cell.…”

Section: Discussionmentioning

confidence: 99%

Sensitivity of Lavender to Proton, Electron, and Gamma Radiation

Chen,

Li,

Shi

et al. 2016

HST

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While ion beams are widely used in plant breeding, little is known about the sensitivity of Lavandula angustifolia (lavender) to ionizing radiation. To compare the biological effects of different types of ionizing radiation on the germination and survival rates of lavender, we exposed lavender seeds to gamma rays, 3 MeV electron beams, and 1.89 MeV proton ion beams. We observed that the seed germination rate decreased with increasing dosages of all three types of ionizing radiation. The malformation rate of lavender seedlings exposed to electron beams and gamma rays increased with increasing radiation dosage. By contrast, the effect of the accelerated proton beams on the malformation rate was negatively correlated with the dosage used. The survival rate of lavender seedlings exposed to the three types of ionizing radiation decreased in a dose-dependent manner. In addition, the survival rate of seedlings irradiated with proton and electron beams decreased more slowly than did that of seedlings irradiated with gamma rays. The half-lethal dose of gamma rays, electron beams, and proton beams was determined to be 48.1 Gy, 134.3 Gy, and 277.8 Gy, respectively, and the most suitable proton-ion energy for lavender seeds in terms of penetration depth was determined to be 5 MeV. These findings provide valuable information for the breeding of lavender by radiation mutation.Additional key words: half-lethal dose, high oil content, mutation breeding of lavender, radiation sensitivity, survival rate nonlinearly related to the LET, with the peak value of the survival curves being 113 keVㆍμm -1 (Tanaka et al., 1997).Proton beams have long been used as low-LET ionizing radiation. The LET values for 9 GeV, 645 MeV, and 50 MeV protons are 0.23, 0.25, and 1.25 keVㆍμm -1 , respectively. These values are close to the LET values of electron beams or gamma rays (value of gamma rays 0.2 keVㆍμm -1 ); however, they are considerably lower than those of heavy-element ion beams. Further, previous studies indicated that low-energy protons (<10 MeV, >4.6 keVㆍμm -1 ) have higher relative biological effectiveness (Yang, 1999). Consequently, low-energy protons can be considered as promising mutagens in plant breeding.Ion beams induce DNA damage and genomic instability in cells by generating reactive oxidative species (Esault et al., 2010;Mughal et al., 2012), which in turn inhibit cell division and cell elongation (Vazquez-Tello, 1996) and suppress tissue regeneration and germination (Zhou et al., 2006;Wu and Yu, 2001). In the context of mutation by ion beams, it has been reported that exposure of roses, carnations, and wheat to ion beams can induce a wide variety of mutations in flower color and flower shape (Okamura et al., 2003;Wu and Yu, 2001). The mechanisms underlying the biological effects of low-energy ion beams on plants may involve a combination of energy deposition, mass deposition, and the exchange of mass and charge (Lin and Yu, 1996).Lavender is widely grown for its essential oils in Asia and Europe. However, the oil content of lave...

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“…The research also demonstrated that the thickness of the seeds’ coat and the seeds’ size were relevant to the proton irradiation priming effect, for example, since 1 MeV ions were not able to penetrate the seed’s coat (≈40 ± 5 µm thickness), it did not affect the seed germination. The seed’s radiative resistance increases with the seed’s coat thickness and the seed’s size, but it decreases with the water content, therefore, the seed’s size and water content should be considered to be a potential selection criterion for seed priming by proton irradiation [ 38 , 118 ]. A representative figure detailing the mode of action carried out by proton beam priming is given in Figure 5 B.…”

Section: Ionizing Physical Seed Priming and The Underlying Mechanismsmentioning

confidence: 99%

Ionizing Radiation: Effective Physical Agents for Economic Crop Seed Priming and the Underlying Physiological Mechanisms

Wang

Zhang

Zhou

et al. 2022

IJMS

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To overcome various factors that limit crop production and to meet the growing demand for food by the increasing world population. Seed priming technology has been proposed, and it is considered to be a promising strategy for agricultural sciences and food technology. This technology helps to curtail the germination time, increase the seed vigor, improve the seedling establishment, and enhance the stress tolerance, all of which are conducive to improving the crop yield. Meanwhile, it can be used to reduce seed infection for better physiological or phytosanitary quality. Compared to conventional methods, such as the use of water or chemical-based agents, X-rays, gamma rays, electron beams, proton beams, and heavy ion beams have emerged as promising physics strategies for seed priming as they are time-saving, more effective, environmentally friendly, and there is a greater certainty for yield improvement. Ionizing radiation (IR) has certain biological advantages over other seed priming methods since it generates charged ions while penetrating through the target organisms, and it has enough energy to cause biological effects. However, before the wide utilization of ionizing priming methods in agriculture, extensive research is needed to explore their effects on seed priming and to focus on the underlying mechanism of them. Overall, this review aims to highlight the current understanding of ionizing priming methods and their applicability for promoting agroecological resilience and meeting the challenges of food crises nowadays.

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Energy related germination and survival rates of water-imbibed Arabidopsis seeds irradiated with protons

Cited by 6 publications

References 9 publications

Biological effects of protons targeted to different ranges in Arabidopsis seeds

Biological effects of protons targeted to different ranges in Arabidopsis seeds

Sensitivity of Lavender to Proton, Electron, and Gamma Radiation

Ionizing Radiation: Effective Physical Agents for Economic Crop Seed Priming and the Underlying Physiological Mechanisms

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