Energy response and fatty acid metabolism in Onychostoma macrolepis exposed to low-temperature stress

Deng, Wei; Sun, Jian; Chang, Zhiguang; Gou, Nina; Wu, Weite; Luo, Xiaolong; Zhou, Jishu; Yu, Haibo; Ji, Hong

doi:10.1016/j.jtherbio.2020.102725

Cited by 25 publications

(10 citation statements)

References 47 publications

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“…In contrast, SFA was the predominant fatty acid used by grass carp during overwintering (Sun et al, 2021). Previous research reported that SFA might be accumulated while MUFA could be utilized to supply energy during cold acclimation in O. macrolepis (Deng et al, 2020). Arslan et al (2020) found that SFA and MUFA were the primary fatty acid sources used by brown trout, Salmo trutta , during a 42‐day starvation.…”

Section: Discussionmentioning

confidence: 99%

“…When the water temperature drops to 10 C, O. macrolepis begin to overwinter (Zhang, 1986). Previous study evaluated energy responses and fatty acid metabolism in O. macrolepis under short-term (48 h) exposure to low temperatures (Deng et al, 2020). As far as we know, there has been no report done to reveal the lipid metabolism characteristics, physiological changes, and antioxidant status of O. macrolepis during overwintering.…”

Section: Introductionmentioning

confidence: 99%

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Effects of overwintering on proximate composition, fatty acid composition, serum parameters, antioxidant status, and lipid metabolism in Onychostoma macrolepis

Gou

Wang

Jin

et al. 2023

J World Aquaculture Soc

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To investigate the metabolic adaptation and lipid utilization to overwintering in Onychostoma macrolepis (27.05 ± 1.79 g), fish were sampled at 0, 75, and 150 days, respectively. Fish were randomly reared into three outdoor tanks (water temperature range:0–10°C). After overwintering, body weight, viscera index, hepatopancreas index, and intraperitoneal fat index were significantly lower than before (p < 0.05). In liver, the contents of monounsaturated fatty acid and polyunsaturated fatty acid (PUFA) were observed to be the lowest and the highest after 150 days of overwintering, respectively (p < 0.05). In serum, the concentrations of triglyceride (TG) and total cholesterol significantly decreased while the activities of alanine aminotransferase and aspartate aminotransferase significantly increased at Day 75 (p < 0.05). Hepatic antioxidant activities were significantly lower whereas malondialdehyde content was significantly higher at Day 150 (p < 0.05). Serum antioxidant indexes were similar to those in liver. During overwintering, the decrease of TG contents in tissues was influenced by the upregulation of lipidolysis genes. These results indicate that lipids were continuously mobilized as physiological fuels throughout overwintering. Overwintering could result in poor antioxidant status and higher lipid peroxidation level in O. macrolepis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of overwintering on proximate composition, fatty acid composition, serum parameters, antioxidant status, and lipid metabolism in Onychostoma macrolepis

Gou

Wang

Jin

et al. 2023

J World Aquaculture Soc

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“…Combined with the tissue analyses, we hypothesize that the decrease in lipid droplets could be associated with increased lipolysis of the higher molecular weight lipids, resulting in increased levels of fatty acids [ 87 ]. Then, SFAs and MUFAs could be used for oxidative metabolism to produce energy [ 71 , 88 ], and PUFAs could be used as a component of biological membranes [ 71 – 73 ]. We also found several cold-induced DEGs involved in fatty acid elongation and biosynthesis.…”

Section: Discussionmentioning

confidence: 99%

Physiological, metabolomic, and transcriptomic reveal metabolic pathway alterations in Gymnocypris przewalskii due to cold exposure

Liu,

Tian,

et al. 2023

BMC Genomics

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Teleost fish have evolved various adaptations that allow them to tolerate cold water conditions. However, the underlying mechanism of this adaptation is poorly understood in Tibetan Plateau fish. RNA-seq combined with liquid chromatography‒mass spectrometry (LC‒MS/MS) metabolomics was used to investigate the physiological responses of a Tibetan Plateau-specific teleost, Gymnocypris przewalskii, under cold conditions. The 8-month G. przewalskii juvenile fish were exposed to cold (4 ℃, cold acclimation, CA) and warm (17 ℃, normal temperature, NT) temperature water for 15 days. Then, the transcript profiles of eight tissues, including the brain, gill, heart, intestine, hepatopancreas, kidney, muscle, and skin, were evaluated by transcriptome sequencing. The metabolites of the intestine, hepatopancreas, and muscle were identified by LC‒MS/MS. A total of 5,745 differentially expressed genes (DEGs) were obtained in the CA group. The key DEGs were annotated using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis. The DEGs from the eight tissues were significantly enriched in spliceosome pathways, indicating that activated alternative splicing is a critical biological process that occurs in the tissues to help fish cope with cold stress. Additionally, 82, 97, and 66 differentially expressed metabolites were identified in the intestine, hepatopancreas, and muscle, respectively. Glutathione metabolism was the only overlapping significant pathway between the transcriptome and metabolome analyses in these three tissues, indicating that an activated antioxidative process was triggered during cold stress. In combination with the multitissue transcriptome and metabolome, we established a physiology-gene‒metabolite interaction network related to energy metabolism during cold stress and found that gluconeogenesis and long-chain fatty acid metabolism played critical roles in glucose homeostasis and energy supply.

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“…High exercise and crowding stress reduce the lipid deposition in fish meat because they demand more energy for muscular and metabolic activity (dos Santos et al, 2019). Under these conditions, the fish obtain energy from lipids and protein (Costas et al, 2008; Deng et al, 2020; Sokolova et al, 2012). It is known that animals and omnivorous fish store energy mainly in the form of neutral lipids in the adipose tissue (Klepsatel et al, 2016; Tolussi et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…The lowest proportion of SFA in the HSD could be explained in terms of catabolism. The catabolism of SFA yields more energy per same length of fatty acid than metabolizing MUFA, PUFA or HUFA (Deng et al, 2019, 2020). The higher content of SFA in the LSD group was represented by C14:0 and C16:0.…”

Section: Discussionmentioning

confidence: 99%

Effects of stocking density on productive performance, economic profit and muscle chemical composition of pacu ( Piaractus mesopotamicus H) cultured in floating cages

Montenegro

Cunzolo

Preussler

et al. 2022

Aquaculture Research

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The present 360‐day study aimed to evaluate the productive performance, economic profit and meat quality of pacu cultured in floating cages at different stocking densities. A total of 2880 juvenile pacu, initial weight 50 ± 0.9 g, were reared in eight floating cages of 18.375 m3. Two stoking densities were used: high stocking density (HSD; 26 fish/m3) and low stocking density (LSD; 13 fish/m3). On days 0, 90, 180, 270 and 360, 100 fish per group were used to determine the growth performance, productive indices and total economic profit. The total economic profit was calculated by indices that include the fish price, feed conversion ratio and diet cost, among others. On day 360, the chemical composition and fatty acid profile of the muscle tissues were determined. The LSD group had higher final biomass and weight gain than the HSD group (p = 0.0001 and p < 0.0001 respectively), which was probably associated with the higher protein efficiency ratio obtained in the LSD group (p < 0.05). Additionally, the economic profit from LSD on day 360 was 1.2 times higher than that from HSD (p < 0.0001). More protein and lipid contents were obtained from LSD (p = 0.0035 and p < 0.0001 respectively) while more moisture was obtained from the HSD (p < 0.0001) group. In addition, more eicosapentaenoic (EPA) (p = 0.0013), docosapentaenoic (DPA) (p = 0.0001) and docosahexaenoic (DHA) (p = 0.0003) fatty acids were obtained from LSD. We concluded that when pacu are cultured in floating cages, 13 fish/m3 density produce fish with more weight and better muscle chemical composition, improving the economic profit and the nutritional quality.

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Energy response and fatty acid metabolism in Onychostoma macrolepis exposed to low-temperature stress

Cited by 25 publications

References 47 publications

Effects of overwintering on proximate composition, fatty acid composition, serum parameters, antioxidant status, and lipid metabolism in Onychostoma macrolepis

Effects of overwintering on proximate composition, fatty acid composition, serum parameters, antioxidant status, and lipid metabolism in Onychostoma macrolepis

Physiological, metabolomic, and transcriptomic reveal metabolic pathway alterations in Gymnocypris przewalskii due to cold exposure

Effects of stocking density on productive performance, economic profit and muscle chemical composition of pacu ( Piaractus mesopotamicus H) cultured in floating cages

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