Energy substrate requirements for in-vitro development of hamster 1- and 2-cell embryos to the blastocyst stage

McKiernan, Susan H.; Bavister, Barry D.; Tasca, Richard J.

doi:10.1093/oxfordjournals.humrep.a137260

Cited by 108 publications

(62 citation statements)

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“…The superiority of CZB to WM as a medium for mastomys embryo culture may be attributed to the presence of glutamine. Similar beneficial effects of glutamine on embryonic development were reported in hamster [15], pig [16], sheep [17], and a certain blocking mouse strain [7]. Glutamine is known to be used by preimplantation mammalian embryos as sources of energy or amino groups [18].…”

Section: Discussionsupporting

confidence: 54%

Preimplantation Embryo Development in Mastomys (Praomys coucha) In Vivo and In Vitro.

Ogura

Matsuda

Mochida

et al. 1997

Journal of Reproduction and Development

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Abstract. Mastomys (Praomys coucha) is a small murine rodent native in Africa. The present study was undertaken to investigate the developmental time course of mastomys embryos in vivo and the rate of development in vitro. Mastomys embryos developing in vivo exhibited a long 2-cell stage, which lasted from late Day 1 through Day 3. At Day 4, most embryos developed to the 8-cell or morula stages and at Day 5, all embryos reached the blastocyst stage. The attachment of embryos to the endometrium began at Day 5, as revealed by histological examinations. The development in vitro was investigated in different media with three inbred strains and an F1 hybrid. When 1-cell embryos from inbred mastomys were cultured in Whitten's medium (WM), none of them (n=61) developed beyond the 2-cell stage. Embryos of the same strains collected at Day 2 (2-cells) reached the blastocyst stage (46%, 31/67) in the same medium. Meanwhile, 1-cell embryos from F1 hybrid mastomys developed to the blastocyst stage in WM (13%, 13/71), indicating the presence of a strong heterosis effect. CZB medium, which lacks glucose and contains glutamine and EDTA, supported the development of 1-cell inbred embryos to the blastocyst stage (24%, 17/70). Supplementation of glutamine, but not EDTA, to WM stimulated the development of 1-cells to the blastocyst stage (22%, 11/51). These findings indicate that the development of mastomys embryos in vitro is both strain and medium dependent and that glutamine may play a crucial role in early development of mastomys embryos.

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Section: Discussionsupporting

confidence: 54%

Preimplantation Embryo Development in Mastomys (Praomys coucha) In Vivo and In Vitro.

Ogura

Matsuda

Mochida

et al. 1997

Journal of Reproduction and Development

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“…Chemically defined media have been developed for IVM and IVC in rodents and domestic animals (McKiernan et al, 1991;Keskintepe and Brackett, 1996;McKiernan and Bavister, 2000;Kishida et al, 2004;Oyamada et al, 2004). Recent work reported the success of two chemically defined media (CMRL-1066 and HECM-10) in supporting maturation of rhesus monkey oocytes in vitro (Zheng et al, 2001a).…”

Section: Serum-free Chemically Defined Medium For Ivm Of Rhesus Monkementioning

confidence: 99%

“…Energy substrates have been found to affect oocyte maturation and embryo development in a wide variety of mammals (McKiernan et al, 1991;Rose-Hellekant et al, 1998;Zheng et al, 2001c). Glucose, lactate and pyruvate are designated as the "energy substrate triad" in cellular metabolism (Bavister, 1995).…”

Section: Effect Of Energy Substrates On Development Potential Of Rhesmentioning

confidence: 99%

Effects of in vitro maturation of monkey oocytes on their developmental capacity

Zheng

2007

Animal Reproduction Science

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The study of in vitro maturation (IVM) of rhesus monkey oocytes has important implications for biomedical research and human infertility treatment. In vitro-matured rhesus monkey oocytes show much less developmental potential than IVM oocytes of other species. Since about 1980 when rhesus monkey IVM, in vitro fertilization (IVF) and in vitro embryo culture (IVC) systems were established, numerous efforts have been made to improve the developmental competence of oocytes and to understand the mechanisms regulating oocyte maturation. This review describes recent progress in this area, particularly the effects of factors such as steroid hormones, energy substrates, amino acids, ovarian follicle status, maternal age and breeding season on the developmental competence, gene expression patterns and genome integrity of rhesus IVM oocytes.

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“…In order to control the variance derived from individual animals, the COCs from individual females were equally assigned to all the treatment groups in experiments 2 and 3 (controlled pooling) [16]. Percentage data were transformed by arcsin transformation (Tukey-Freeman transformation) [17], and nuclear numbers, by log transformation.…”

Section: Experimental Design and Statistical Analysismentioning

confidence: 99%

Kinetics of In Vitro Fertilization and Development of an Inbred Mouse Strain: A Study Comparing RFM/Ms with C57BL/6J

Kito¹,

Tateno

Ohta

et al. 2002

J.Mamm.Ova.Res.

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In vitro fer tilizability and subsequent developmental competence of inbred RFM/Ms mice, which have high incidence of myeloid leukemia by radiation, was studied. When response of RFM/Ms females to various amount of eCG (1.25-10.0 i.u.) In the past few decades, the development of techniques such as in vitro fertilization (IVF) and in vitro embryo culture (IVC) for many mammalian species has significantly contributed to clinical intervention in human and endangered species infertility, as well as the animal science of domesticated and laboratory animals [1]. Of these mammals, mice are most frequently used as models for reproductive physiological studies. Although many mouse strains have been bred for specific purposes of research, their reproductive profiles vary among strains [2]. In vitro handling, such as IVF and IVC is not necessarily possible in all inbred strains of mice [3][4][5][6]. The feasibility of IVF and IVC of a particular mouse strain has to be determined on a strain by strain basis in most cases.We report the ability to manipulate gametes and embryos from an inbred RFM/Ms mouse strain that has often been used in the study of leukemia [7,8]. In spite of the importance of this strain in radiation biology and immunology due to its high incidence of myeloid le uke m ia a ft er r adi at io n exp osu re [9,10 ], t he reproductive profile and gamete/zygote handling in vitro have not been studied. Therefore, we examined this strain's ovulatory response to hormone stimulation and the fertilization and developmental competence of RFM/ Ms gametes and zygotes in IVF and IVC including comparison with C57BL/6J gametes and embryos. As we observed differences in the kinetics of fertilization and embryo development, an attempt was made to investigate how paternal and maternal factors affect fertilization and developmental kinetics in vitro by producing hybrid embryos. Materials and Methods AnimalsInbred RFM/Ms and C57BL/6J mice have been maintained by sib mating over 80 generations in the specific pathogen free animal facility of the National

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Energy substrate requirements for in-vitro development of hamster 1- and 2-cell embryos to the blastocyst stage

Cited by 108 publications

References 0 publications

Preimplantation Embryo Development in Mastomys (Praomys coucha) In Vivo and In Vitro.

Preimplantation Embryo Development in Mastomys (Praomys coucha) In Vivo and In Vitro.

Effects of in vitro maturation of monkey oocytes on their developmental capacity

Kinetics of In Vitro Fertilization and Development of an Inbred Mouse Strain: A Study Comparing RFM/Ms with C57BL/6J

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