Energy transfer and trapping in photosynthesis

Grondelle, Rienk van; Dekker, Jan; Gillbro, Tomas; Sundström, Villy

doi:10.1016/0005-2728(94)90166-x

Cited by 1,072 publications

(880 citation statements)

References 488 publications

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“…This confirms that the two main emission bands of PSII at 77 K (F-685 and F-695) have very different origins (Andrizhiyevskaya et al 2005). Similarly, the DAS of PSI-LHCI clearly show the presence of two different pools of red pigments, with emission at 718 and 733 nm, which are respectively located in the core complex and light-harvesting antennae (Van Grondelle et al 1994). The red pigments of the PSI core are seen to become populated in the early timescale (9 ps), whereas the emission from the red pigments in LHCI arises relatively slowly at the timescale of 59 ps (Fig.…”

Section: K Time-resolved Fluorescencesupporting

confidence: 67%

Excitation energy transfer in native and unstacked thylakoid membranes studied by low temperature and ultrafast fluorescence spectroscopy

et al. 2007

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In this work, the transfer of excitation energy was studied in native and cation-depletion induced, unstacked thylakoid membranes of spinach by steady-state and time-resolved fluorescence spectroscopy. Fluorescence emission spectra at 5 K show an increase in photosystem I (PSI) emission upon unstacking, which suggests an increase of its antenna size. Fluorescence excitation measurements at 77 K indicate that the increase of PSI emission upon unstacking is caused both by a direct spillover from the photosystem II (PSII) core antenna and by a functional association of light-harvesting complex II (LHCII) to PSI, which is most likely caused by the formation of LHCII-LHCI-PSI supercomplexes. Time-resolved fluorescence measurements, both at room temperature and at 77 K, reveal differences in the fluorescence decay kinetics of stacked and unstacked membranes. Energy transfer between LHCII and PSI is observed to take place within 25 ps at room temperature and within 38 ps at 77 K, consistent with the formation of LHCII-LHCI-PSI supercomplexes. At the 150-160 ps timescale, both energy transfer from LHCII to PSI as well as spillover from the core antenna of PSII to PSI is shown to occur at 77 K. At room temperature the spillover and energy transfer to PSI is less clear at the 150 ps timescale, because these processes compete with charge separation in the PSII reaction center, which also takes place at a timescale of about 150 ps.

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Section: K Time-resolved Fluorescencesupporting

confidence: 67%

Excitation energy transfer in native and unstacked thylakoid membranes studied by low temperature and ultrafast fluorescence spectroscopy

et al. 2007

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“…Decomposition analysis of the fluorescence spectra yielded a best fit with six spectral components (Fig. 2, Table 2) corresponding to the emission of three sub-bands from PSII (678-681, 687-690 and 699-701 nm), two sub-bands from PSI (721-722 and 735-738 nm) (Krause and Weis, 1984) and a spectral component centered around 740-760 nm corresponding to vibrational transitions (F vib ) in the near-infrared region (Van Grondelle et al, 1994). Although the overall composition of spectral sub-bands in both WT and F2 mutant thylakoid membranes was similar, the area ratio of the sum of PSII-and PSI-related components is significantly higher in F2 mutant (0.71) compared to WT chloroplasts (0.31) ( Table 2).…”

Section: Consistent With Previous Reports the Quantitative Analysis Omentioning

confidence: 99%

The lack of LHCII proteins modulates excitation energy partitioning and PSII charge recombination in Chlorina F2 mutant of barley

Ivanov

Król

Zeinalov

et al. 2008

Physiol Mol Biol Plants

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Analysis of the partitioning of absorbed light energy within PSII into fractions utilized by PSII photochemistry (Ø PSII ), thermally dissipated via ∆pH-and zeaxanthin-dependent energy quenching (Ø NPQ ) and constitutive non-photochemical energy losses (Ø NO ) was performed in wild type and F2 mutant of barley. The estimated energy partitioning of absorbed light to various pathways indicated that the fraction of Ø PSII was slightly higher, while the proportion of thermally dissipated energy through Ø NPQ was 38% lower in F2 mutant than in WT. In contrast, Ø NO , i.e. the fraction of absorbed light energy dissipated by additional quenching mechanism(s) was 34% higher in F2 mutant. The increased proportion of Ø NO correlated with narrowing the temperature gap (∆T M ) between S 2/3 Q B -and S 2 Q A -charge recombinations in F2 mutant as revealed by thermoluminescence measurements. We suggest that this would result in increased probability for an alternative non-radiative P680 + Q A -radical pair recombination pathway for energy dissipation within the reaction centre of PSII (reaction center quenching) and that this additional quenching mechanism might play an important role in photoprotection when the capacity for the primary, zeaxanthin-dependent non-photochemical quenching ( Research ArticleChanges in irradiance, temperature, nutrient and water availability result in imbalances between the light energy absorbed through photochemistry and energy utilization through photosynthetic electron transport coupled to carbon, nitrogen and sulphur reduction. Such an imbalance caused by changes in irradiance and/or temperature, nutrient and water availability leads to photoinhibition of photosynthesis that may result in photodamage to the D1 reaction centre polypeptide of PSII (Krause, 1988;Aro et al., 1993). The major mechanism for thermal de-excitation of excess light energy in higher plants is currently considered to be the ∆pH-and xanthophyll-cycle dependent nonphotochemical quenching (NPQ) occurring within LHCII antenna pigment bed of PSII (Demmig-Adams and Adams, 1992;Horton et al., 1996). The role of pH-and zeaxanthin-dependent shifts in the oligomerization state of LHCII in developing the rapidly relaxing energy dependent component (qE) of NPQ is well established with qE representing the major protective mechanism against photoinhibitory damage of PSII (Horton et al., 1996; Niyogi, 1999). It has been demonstrated that trimers of LHCII exhibit the optimum level of non-photochemical energy dissipation by modulating the development of the quenched state of the complex (Wentworth et al., 2004).The redox-dependent reversible phosphorylation of light-harvesting Chl a/b-binding proteins (LHCII) is the

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“…1D). This not only significantly increases the absorption spectral window but also creates an energy gradient, so that the excitation energy can be "funnelled" to a particular site in the LHC or to the reaction centre [9,10]. Another notable property of Chls is their electronic excited states having intrinsic decay times of a few ns.…”

Section: Introductionmentioning

confidence: 99%

Design principles of natural light-harvesting as revealed by single molecule spectroscopy

Krüger

Grondelle

2016

Physica B: Condensed Matter

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Biology offers a boundless source of adaptation, innovation, and inspiration. A wide range of photosynthetic organisms exist that are capable of harvesting solar light in an exceptionally efficient way, using abundant and low-cost materials. These natural light-harvesting complexes consist of proteins that strongly bind a high density of chromophores to capture solar photons and rapidly transfer the excitation energy to the photochemical reaction centre. The amount of harvested light is also delicately tuned to the level of solar radiation to maintain a constant energy throughput at the reaction centre and avoid the accumulation of the products of charge separation. In this Review, recent developments in the understanding of light harvesting by plants will be discussed, based on results obtained from single molecule spectroscopy studies. Three design principles of the main light-harvesting antenna of plants will be highlighted: (a) fine, photoactive control over the intrinsic protein disorder to efficiently use intrinsically available thermal energy dissipation mechanisms; (b) the design of the protein microenvironment of a low-energy chromophore dimer to control the amount of shade absorption; (c) the design of the exciton manifold to ensure efficient funneling of the harvested light to the terminal emitter cluster.

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Energy transfer and trapping in photosynthesis

Cited by 1,072 publications

References 488 publications

Excitation energy transfer in native and unstacked thylakoid membranes studied by low temperature and ultrafast fluorescence spectroscopy

Excitation energy transfer in native and unstacked thylakoid membranes studied by low temperature and ultrafast fluorescence spectroscopy

The lack of LHCII proteins modulates excitation energy partitioning and PSII charge recombination in Chlorina F2 mutant of barley

Design principles of natural light-harvesting as revealed by single molecule spectroscopy

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