Energy transfer between a biological labelling dye and gold nanorods

Racknor, Chris; Singh, Mahi R.; Zhang, Yinan; Birch, David J. S.; Chen, Yu

doi:10.1088/2050-6120/2/1/015002

Cited by 30 publications

(26 citation statements)

References 39 publications

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“…[38][39][40][41][42][43][44][45][46] Understanding the exact location of gold NCs within serum albumin is important to any future work to improve the fluorescent properties of these fluorophores; knowing the location will allow the alteration of the protein in ways whereby the fluorescent properties of the gold can be controlled. The simulation showed that gold NCs have a strong tendency to bind to hydrophobic pockets on the protein surface, which traps them close to cysteine residues for subsequent chemical interaction.…”

Section: Discussionmentioning

confidence: 99%

Locating the nucleation sites for protein encapsulated gold nanoclusters: a molecular dynamics and fluorescence study

Russell

Kubiak-Ossowska

Mulheran

et al. 2015

Phys. Chem. Chem. Phys.

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Fluorescent gold nanoclusters encapsulated by proteins have attracted considerable attention in recent years for their unique properties as new fluorescence probes for biological sensing and imaging. However, fundamental questions, such as the nucleation sites of gold nanoclusters within proteins and the fluorescence mechanism remain unsolved. Here we present a study of the location of gold nanoclusters within bovine serum albumin (BSA) combining both fully atomistic molecular dynamic (MD) simulations and fluorescence spectroscopic studies. The MD simulations show gold clusters growing close to a number of cysteine sites across all three domains of BSA, although just two major sites in domains IIB and IA were found to accommodate large clusters comprising more than 12 atoms. The dependence of the fluorescence on pH is found to be compatible with possible nucleation sites in domains IIB and IA. Furthermore, the energy transfer between tryptophan and gold nanoclusters reveals a separation of 29.7 Å, further indicating that gold nanoclusters were most likely located in the major nucleation site in domain IIB. The disclosure of the precise location of the gold nanoclusters and their surrounding amino acid residues should help better understanding of their fluorescence mechanism and aid their optimization as fluorescent nanoprobes.

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Section: Discussionmentioning

confidence: 99%

Locating the nucleation sites for protein encapsulated gold nanoclusters: a molecular dynamics and fluorescence study

Russell

Kubiak-Ossowska

Mulheran

et al. 2015

Phys. Chem. Chem. Phys.

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“…Enhanced energy transfer, indicated by a large decrease in the fluorescence lifetime of fluorophore, was observed when the excitation wavelength matched the longitudinal surface plasmon band of the gold cores. Further energy transfer between Alexa Fluor 405 and gold nanorods under one-and two-photon excitation was demonstrated experimentally using FLIM and theoretically using density matrix method [21]. The energy transfer via the dipole-dipole interaction was found to cause a decrease in the fluorescence lifetime.…”

Section: Introductionmentioning

confidence: 92%

Surface plasmon enhanced energy transfer between gold nanorods and fluorophores: application to endocytosis study and RNA detection

Zhang

Wei

et al. 2015

Faraday Discuss.

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Previously we have demonstrated surface plasmon enhanced energy transfer between fluorophores and gold nanorods under two-photon excitation using fluorescence lifetime imaging microscopy (FLIM) in both solution and intracellular phases. These studies demonstrated that gold nanoparticle-dye energy transfer combinations are appealing, not only in Förster resonance energy transfer (FRET) imaging, but also energy transfer-based fluorescence lifetime sensing of bio-analytes. Here, we apply this approach to study the internalization of gold nanorods (GNRs) in HeLa cells using the early endosome labeling marker GFP. The observed energy transfer between GFP and the GNRs indicates the involvement of endocytosis in GNR uptake. Moreover, a novel nanoprobe based on oligonucleotide functionalized gold nanorods for nucleic acid sensing via dye-GNRs energy transfer is demonstrated, potentially opening up new possibilities in cancer diagnosis and prognosis. The influence of oligonucleotide design on such nanoprobe performance was studied for the first time using time-resolved fluorescence spectroscopy, bringing new insights to the optimization of the nanoprobe.

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“…[25,26,27] for a recent although non extensive list of works on this topic). The plasmonic properties of a nanostructure depend dramatically on its size and shape, as has been demonstrated in studies of nanorods, [28,29,30] nanocubes, [31,32] nanostars, [33] and numerous other structures [22]. Between these structures, single plasmonic dimers consisting of adjacent nanoparticle pairs, give rise to very large field corrections in their junctions, when the surface plasmons are excited [34] which make them highly attractive as SERS substrates, with enhancements approaching single-molecule sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Spin initialization of a p-doped quantum dot coupled to a bowtie nanoantenna

Carreño

Arrieta-Yáñez

Antón

2015

Optics Communications

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The spin initialization of a hybrid system consisting of a p-doped semiconductor quantum dot coupled to a gold bowtie nanoantenna is analyzed. The quantum dot is described as a four-level atom-like system using the density matrix formalism. The two lower levels are Zeeman-split hole spin states and the two upper levels correspond to positively charged excitons with a spin-up, spin-down hole pair and opposite spin electron. The gold bowtie nanoantenna is placed in close proximity to the quantum dot. A linearly polarized laser field drives two of the optical transitions of the quantum dot and produces localized surface charge oscillations in the nanoantenna which act back upon the quantum dot thus changing the effective field felt by it. The angular frequencies of those charge oscillations are very different along its two principal axes, resulting in an anisotropic modification of the spontaneous emission rates of the allowed optical transitions of the quantum dot. These changes are accounted for by using the Green tensor method, and result in a faster spin state initialization than that of the isolated quantum dot. We also show that the presence of the nanoantenna dramatically modifies the optical properties of the fluorescent photons, either in the spectral and in the time domain.

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Energy transfer between a biological labelling dye and gold nanorods

Cited by 30 publications

References 39 publications

Locating the nucleation sites for protein encapsulated gold nanoclusters: a molecular dynamics and fluorescence study

Locating the nucleation sites for protein encapsulated gold nanoclusters: a molecular dynamics and fluorescence study

Surface plasmon enhanced energy transfer between gold nanorods and fluorophores: application to endocytosis study and RNA detection

Spin initialization of a p-doped quantum dot coupled to a bowtie nanoantenna

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