Precision Livestock Farming (PLF) is being developed in livestock farms to relieve the human workload and to help farmers to optimize production and management procedure. The objectives of this study were to evaluate the consequences in energy intensity and the related carbon impact, from dairy farm to cheese factory, due to the implementation of a real-time milk analysis and separation (AfiMilk MCS) in milking parlors. The research carried out involved three conventional dairy farms, the collection and delivery of milk from dairy farms to cheese factory and the processing line of a traditional soft cheese into a dairy factory. The AfiMilk MCS system installed in the milking parlors allowed to obtain a large number of information related to the quantity and quality of milk from each individual cow and to separate milk with two different composition (one with high coagulation properties and the other one with low coagulation properties), with different percentage of separation. Due to the presence of an additional milkline and the AfiMilk MCS components, the energy requirements and the related environmental impact at farm level were slightly higher, among 1.1% and 4.4%. The logistic of milk collection was also significantly reorganized in view of the collection of two separate type of milk, hence, it leads an increment of 44% of the energy requirements. The logistic of milk collection and delivery represents the process which the highest incidence in energy consumption occurred after the installation of the PLF technology. Thanks to the availability of milk with high coagulation properties, the dairy plant, produced traditional soft cheese avoiding the standardization of the formula, as a result, the energy uses decreased about 44%, while considering the whole chain, the emissions of carbon dioxide was reduced by 69%. In this study, the application of advance technologies in milking parlors modified not only the on-farm management but mainly the procedure carried out in cheese making plant. This aspect makes precision livestock farming implementation unimportant technology that may provide important benefits throughout the overall milk chain, avoiding about 2.65 MJ of primary energy every 100 kg of processed milk.