Engineered 3D printed poly(ɛ-caprolactone)/graphene scaffolds for bone tissue engineering

Wang, Weiguang; Passarini, José Roberto; Nalesso, Paulo Roberto Lopes; Musson, David S.; Cornish, Jillian; Mendonça, Fernanda Aparecida Sampaio; Caetano, Guilherme Ferreira; Bartolo, Paulo Jorge Da Silva

doi:10.1016/j.msec.2019.03.047

Cited by 108 publications

(114 citation statements)

References 51 publications

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“…Despite EStim's demonstrated effectiveness improving bone healing, both in pre-clinical animal studies and in clinical settings, few studies have investigated the effectiveness of combining EStim with BTE treatments in vivo [27, 29, 94,147,148]. In one of these studies, our group treated large defects in rat femurs with AT-MSC + Scaffold + EStim.…”

Section: Clinical Estim Bone Treatment Devicesmentioning

confidence: 99%

Electrical stimulation in bone tissue engineering treatments

Leppik

Oliveira

Bhavsar

et al. 2020

Eur J Trauma Emerg Surg

161

148

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Electrical stimulation (EStim) has been shown to promote bone healing and regeneration both in animal experiments and clinical treatments. Therefore, incorporating EStim into promising new bone tissue engineering (BTE) therapies is a logical next step. The goal of current BTE research is to develop combinations of cells, scaffolds, and chemical and physical stimuli that optimize treatment outcomes. Recent studies demonstrating EStim's positive osteogenic effects at the cellular and molecular level provide intriguing clues to the underlying mechanisms by which it promotes bone healing. In this review, we discuss results of recent in vitro and in vivo research focused on using EStim to promote bone healing and regeneration and consider possible strategies for its application to improve outcomes in BTE treatments. Technical aspects of exposing cells and tissues to EStim in in vitro and in vivo model systems are also discussed.

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Section: Clinical Estim Bone Treatment Devicesmentioning

confidence: 99%

Electrical stimulation in bone tissue engineering treatments

Leppik

Oliveira

Bhavsar

et al. 2020

Eur J Trauma Emerg Surg

161

148

View full text Add to dashboard Cite

show abstract

“…The PANI powder (after ball milling) was mixed with the melted PCL (~80 • C) by manual physical melt blending for a minimum of 30 min, based on a previously described method [12][13][14], to ensure a homogenous PCL/PANI blend with PANI filler loading concentrations of 0.1; 1; 2% wt. The PCL/PANI blends were allowed to cool and then cut into small pieces to enable loading into the material chamber of the 3D printer.…”

Section: Fabrication Of Polyaniline-polycaprolactone Scaffoldsmentioning

confidence: 99%

“…This new class of smart electroactive biomaterials that are able to deliver electrical, electrochemical or electromechanical stimulation directly to cells, tissue, and organs could bring a significant breakthrough in the tissue engineering field [3,5,6]. Electroactive scaffolds have already been investigated for use in neural [4,5,9], muscle [10,11], bone [12][13][14], and cardiac [15,16] tissue engineering applications. A key requirement is the development of biocompatible, biodegradable, and electrically conductive biomaterials.…”

Section: Introductionmentioning

confidence: 99%

“…Electroactive scaffolds can be fabricated from conductive materials or prepared by the addition of conductive fillers to create a composite. These conductive materials can be classified as: carbon-based [5,12,14,15], conductive polymers [1][2][3]6], and metallic-based [16][17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

“…However, these methods offer limited control of scaffold morphological properties such as pore size, interconnectedness, and fibre diameter. By contrast, 3D printing enables the precise control of scaffold morphology with the production of interconnected structures and a regular pore size [12][13][14]. 3D printing has been utilised in a range of tissue engineering applications such as cartilage [47], bone [48], vasculature [49], and nerve [50] and facilitates the fabrication of multimaterial and geometrical complex architectures that more accurately reflects that native in vivo environment [51].…”

Section: Introductionmentioning

confidence: 99%

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3D Printing of Polycaprolactone–Polyaniline Electroactive Scaffolds for Bone Tissue Engineering

et al. 2020

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Electrostimulation and electroactive scaffolds can positively influence and guide cellular behaviour and thus has been garnering interest as a key tissue engineering strategy. The development of conducting polymers such as polyaniline enables the fabrication of conductive polymeric composite scaffolds. In this study, we report on the initial development of a polycaprolactone scaffold incorporating different weight loadings of a polyaniline microparticle filler. The scaffolds are fabricated using screw-assisted extrusion-based 3D printing and are characterised for their morphological, mechanical, conductivity, and preliminary biological properties. The conductivity of the polycaprolactone scaffolds increases with the inclusion of polyaniline. The in vitro cytocompatibility of the scaffolds was assessed using human adipose-derived stem cells to determine cell viability and proliferation up to 21 days. A cytotoxicity threshold was reached at 1% wt. polyaniline loading. Scaffolds with 0.1% wt. polyaniline showed suitable compressive strength (6.45 ± 0.16 MPa) and conductivity (2.46 ± 0.65 × 10−4 S/cm) for bone tissue engineering applications and demonstrated the highest cell viability at day 1 (88%) with cytocompatibility for up to 21 days in cell culture.

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Scaffold Fabrication Technologies and Structure/Function Properties in Bone Tissue Engineering

Collins

Ren

Young

et al. 2021

Adv Funct Materials

549

254

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Bone tissue engineering (BTE) is a rapidly growing field aiming to create a biofunctional tissue that can integrate and degrade in vivo to treat diseased or damaged tissue. It has become evident that scaffold fabrication techniques are very important in dictating the final structural, mechanical properties, and biological response of the implanted biomaterials. A comprehensive review of the current accomplishments on scaffold fabrication techniques, their structure, and function properties for BTE is provided herein. Different types of biomaterials ranging from inorganic biomaterials to natural and synthetic polymers and related composites for scaffold processing are presented. Emergent scaffolding techniques such as electrospinning, freeze-drying, bioprinting, and decellularization are also discussed. Strategies to improve vascularization potential and immunomodulation, which is considered a grand challenge in BTE scaffolding, are also presented.

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Engineered 3D printed poly(ɛ-caprolactone)/graphene scaffolds for bone tissue engineering

Cited by 108 publications

References 51 publications

Electrical stimulation in bone tissue engineering treatments

Electrical stimulation in bone tissue engineering treatments

3D Printing of Polycaprolactone–Polyaniline Electroactive Scaffolds for Bone Tissue Engineering

Scaffold Fabrication Technologies and Structure/Function Properties in Bone Tissue Engineering

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