Engineered Aligned Endothelial Cell Structures in Tethered Collagen Hydrogels Promote Peripheral Nerve Regeneration

Engineered Neural Tissue Made Using Hydrogels Derived from Decellularised Tissues for the Regeneration of Peripheral Nerves

“…The key finding from this experiment was that B-ECM EngNT is as supportive of nerve regeneration as collagen I derived EngNT. Although the autograft group was superior in terms of axonal regeneration, this was in line with previous work by Muangsanit, Roberton, Costa and Phillips [50] where the experimental groups lie within a spectrum of axonal regeneration provided by the empty conduit and autograft groups at either margin. By increasing the time point of the model, regeneration within the EngNT groups may match that observed in the autograft group.…”

“…A feature which might contribute to the success of Schwann cell implantation is the influence the biomaterial has on encouraging a repair Schwann cell phenotype [49]. In line with previous work [39,50], greater magnitudes of cellular alignment, and subsequent tissue anisotropy, were associated with an elongated, bipolar F7 Schwann cell morphologies; a hallmark of the repair Schwann cell phenotype [49].…”

Endothelial cell-derived exosomes boost and maintain repair-related phenotypes of Schwann cells via miR199-5p to promote nerve regeneration

“…Moreover, SCs secrete several signaling molecules, such as pro-inflammatory factors, cytokines, growth factors, neurotrophic factors and extracellular matrix molecules, to recruit macrophages, support neuronal survival and accelerate axonal regeneration. Finally, remyelinate regenerated axons and repel infiltrated macrophages to complete the process of peripheral nerve repair [2,3].…”

Mesoporous hollow Fe3O4 nanoparticles regulate the behavior of neuro-associated cells through induction of macrophage polarization in an alternating magnetic field

“…We selected two types of cells that play an important role in nerve regeneration: one is neural stem cells (NE-4C cells) that can directly differentiate into neurons and play an important role in promoting nerve repair; [29][30][31] the other is human vascular endothelial cells (HUVECs) that can form blood vessels to guide Schwann cell migration and neuron regeneration. 32 The effect of conditioned culture was reflected in the changes we noted in cell proliferation and migration, which may be more quantifiable, intuitive, and easier to test than other biological indicators.…”