Engineered artificial articular cartilage made of decellularized extracellular matrix by mechanical and IGF-1 stimulation

Sani, Mahsa; Hosseinie, Radmarz; Latifi, Mona; Shadi, Mehri; Razmkhah, Mahboobeh; Salmannejad, Mahin; Parsaei, Hossein; Talaei‐Khozani, Tahereh

doi:10.1016/j.bioadv.2022.213019

Cited by 19 publications

(14 citation statements)

References 39 publications

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“…One study investigated the effect of mechanical stimulation on decellularized ECM for the purpose of cartilage engineering. Chondrocyte‐laden cartilage dECM‐constructs were subjected to mechanical strain for 2 weeks and results indicated that the stimulation increased the expression of chondrogenic markers while also promoting GAG deposition 70 . This study shows exterior mechanical stress and exogenous non‐soluble factors can significantly affect the behavior of cells.…”

Section: Cartilagementioning

confidence: 73%

“…Chondrocyte-laden cartilage dECM-constructs were subjected to mechanical strain for 2 weeks and results indicated that the stimulation increased the expression of chondrogenic markers while also promoting GAG deposition. 70 This study shows exterior mechanical stress and exogenous non-soluble factors can significantly affect the behavior of cells. Another group conducted a study which implanted solubilized articular cartilage derived ECM scaffolds using a 3D printed fixation device.…”

Section: Ecm Derived Materialsmentioning

confidence: 86%

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Cell‐instructive biomaterials in tissue engineering and regenerative medicine

Adhikari

Stager

Krebs

2023

J Biomedical Materials Res

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The field of biomaterials aims to improve regenerative outcomes or scientific understanding for a wide range of tissue types and ailments. Biomaterials can be fabricated from natural or synthetic sources and display a plethora of mechanical, electrical, and geometrical properties dependent on their desired application. To date, most biomaterial systems designed for eventual translation to the clinic rely on soluble signaling moieties, such as growth factors, to elicit a specific cellular response. However, these soluble factors are often limited by high cost, convoluted synthesis, low stability, and difficulty in regulation, making the translation of these biomaterials systems to clinical or commercial applications a long and arduous process. In response to this, significant effort has been dedicated to researching cell‐directive biomaterials which can signal for specific cell behavior in the absence of soluble factors. Cells of all tissue types have been shown to be innately in tune with their microenvironment, which is a biological phenomenon that can be exploited by researchers to design materials that direct cell behavior based on their intrinsic characteristics. This review will focus on recent developments in biomaterials that direct cell behavior using biomaterial properties such as charge, peptide presentation, and micro‐ or nano‐geometry. These next generation biomaterials could offer significant strides in the development of clinically relevant medical devices which improve our understanding of the cellular microenvironment and enhance patient care in a variety of ailments.

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Section: Cartilagementioning

confidence: 73%

Section: Ecm Derived Materialsmentioning

confidence: 86%

Cell‐instructive biomaterials in tissue engineering and regenerative medicine

Adhikari

Stager

Krebs

2023

J Biomedical Materials Res

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“…Following the previously published procedure, hyaline cartilage was collected from the calf nose and decellularized. 22 In brief, minced cartilage was incubated in 10 mMTris-HCl, pH 8.0, then frozen at À80 C and thawed at 37 C for six cycles. Trypsin 0.25% was applied for 24 h at 37 C. After trypsinization of cartilage, it was rinsed with a hypertonic buffer solution containing 150 mMNaCl in 50 mMTris-HCl, pH 7.6, and subsequently treated with 50 U/mL DNAse solution, 10 mMTris-HCl, pH 7.5, for 4 h at 37 C. To vacate enzyme residues, the slurry was treated with hypotonic Tris-HCl for 20 hours, followed by incubation with 1% Triton X-100 for 48 h, and the solution was refreshed every 24 h. PBS was rinsed several times after each step to remove cell remnants and chemicals.…”

Section: Scaffold Preparationmentioning

confidence: 99%

Effect of amniotic membrane/collagen scaffolds on laryngeal cartilage repair

Iravani,

Mousavi,

Owji

et al. 2024

Laryngoscope Investig Oto

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ObjectivesLaryngeal cartilage defects are a major problem that greatly impacts structural integrity and function. Cartilage repair is also a challenging issue. This study evaluated the efficacy of a collagen scaffold enveloped by amniotic membrane (AM/C) on laryngeal cartilage repair.Study DesignExperimental animal study.MethodsFourteen Dutch rabbits were enrolled in the study. A 5 mm cartilage defect was created in the right and left thyroid lamina. The animals were divided into two groups randomly. Group 1 collagen scaffolds and group 2 AM/C were applied to the right side defects. Left side defects were not repaired, serving as control. Histologic evaluation was done 45 and 90 days following collagen and AM/C application with criteria of tissue and cell morphology, lacuna formation, vascularization, and inflammation.ResultsSignificant improvement in cartilage repair was observed in the AM/C side compared to the control side in all histologic criteria after 45 days (p<.05). After 90 days, cartilage repair improved in cell morphology, lacuna formation, and inflammation significantly (p<.05).ConclusionThe combination of amniotic membrane and collagen scaffolds provides a promising treatment modality for improving the repair of laryngeal cartilage defects.Level of EvidenceNA.

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“…As the end product of decellularization, ECM is a non-living, non-cellular biomaterial that supports the resident cells as a particular, functional, and protective substrate ( Rijal, 2017 ; García-Gareta et al, 2020 ; Sani et al, 2022 ). This three-dimensional stroma provides structural and elastic properties, induces necessary biochemical, biophysical, and biomechanical signals for tissue morphogenesis, differentiation, and homeostasis, as well as establishes an appropriate environment for the distribution and transport of nutrients and oxygen to the cells ( Hussey et al, 2018 ; García-Gareta et al, 2020 ; Sani et al, 2022 ). The two main components of the vascular ECM are the basement membrane (BM) and the interstitial ECM.…”

Section: The Role Of Optimized Decellularization In Favorable Endothe...mentioning

confidence: 99%

Angiogenesis and Re-endothelialization in decellularized scaffolds: Recent advances and current challenges in tissue engineering

Mazloomnejad

Babajani

Kasravi

et al. 2023

Front. Bioeng. Biotechnol.

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Decellularization of tissues and organs has recently become a promising approach in tissue engineering and regenerative medicine to circumvent the challenges of organ donation and complications of transplantations. However, one main obstacle to reaching this goal is acellular vasculature angiogenesis and endothelialization. Achieving an intact and functional vascular structure as a vital pathway for supplying oxygen and nutrients remains the decisive challenge in the decellularization/re-endothelialization procedure. In order to better understand and overcome this issue, complete and appropriate knowledge of endothelialization and its determining variables is required. Decellularization methods and their effectiveness, biological and mechanical characteristics of acellular scaffolds, artificial and biological bioreactors, and their possible applications, extracellular matrix surface modification, and different types of utilized cells are factors affecting endothelialization consequences. This review focuses on the characteristics of endothelialization and how to optimize them, as well as discussing recent developments in the process of re-endothelialization.

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Engineered artificial articular cartilage made of decellularized extracellular matrix by mechanical and IGF-1 stimulation

Cited by 19 publications

References 39 publications

Cell‐instructive biomaterials in tissue engineering and regenerative medicine

Cell‐instructive biomaterials in tissue engineering and regenerative medicine

Effect of amniotic membrane/collagen scaffolds on laryngeal cartilage repair

Angiogenesis and Re-endothelialization in decellularized scaffolds: Recent advances and current challenges in tissue engineering

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