Engineered baicalein-decorated zinc phosphates for synergistic alleviation of inflammatory bowel disease by repairing the mucosal barrier and relieving oxidative stress

Liu, Limei; Wu, Weisong; Li, Siqi; Ma, Li; Liu, Yunting; Wang, Xiaoli; Jiang, Yanjun

doi:10.1039/d3bm01284k

Cited by 5 publications

(2 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Zhou et al demonstrated that exogenous Zn-Asp can mitigate damage induced by DON exposure through the regulation of mouse intestinal stem cell renewal and regeneration capacity, thus ameliorating intestinal morphology and barrier function (Zhou et al, 2020). Furthermore, oral administration of ZnBM has been shown to restore the integrity of LPS-damaged Caco-2 cell monolayers and significantly enhance barrier function (Liu L. et al, 2023).…”

Section: Effects Of Zinc Gluconate On the Ultrastructure Of Mouse Ileummentioning

confidence: 99%

Protective effect of zinc gluconate on intestinal mucosal barrier injury in antibiotics and LPS-induced mice

Wang,

Xiao,

Wei

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

ObjectiveThe aim of the study is to investigate the function and mechanism of Zinc Gluconate (ZG) on intestinal mucosal barrier damage in antibiotics and Lipopolysaccharide (LPS)-induced mice.MethodsWe established a composite mouse model by inducing intestinal mucosal barrier damage using antibiotics and LPS. The animals were divided into five groups: Control (normal and model) and experimental (low, medium, and high-dose ZG treatments). We evaluated the intestinal mucosal barrier using various methods, including monitoring body weight and fecal changes, assessing pathological damage and ultrastructure of the mouse ileum, analyzing expression levels of tight junction (TJ)-related proteins and genes, confirming the TLR4/NF-κB signaling pathway, and examining the structure of the intestinal flora.ResultsIn mice, the dual induction of antibiotics and LPS led to weight loss, fecal abnormalities, disruption of ileocecal mucosal structure, increased intestinal barrier permeability, and disorganization of the microbiota structure. ZG restored body weight, alleviated diarrheal symptoms and pathological damage, and maintained the structural integrity of intestinal epithelial cells (IECs). Additionally, ZG reduced intestinal mucosal permeability by upregulating TJ-associated proteins (ZO-1, Occludin, Claudin-1, and JAM-A) and downregulating MLCK, thereby repairing intestinal mucosal barrier damage induced by dual induction of antibiotics and LPS. Moreover, ZG suppressed the TLR4/NF-κB signaling pathway, demonstrating anti-inflammatory properties and preserving barrier integrity. Furthermore, ZG restored gut microbiota diversity and richness, evidenced by increased Shannon and Observed features indices, and decreased Simpson’s index. ZG also modulated the relative abundance of beneficial human gut bacteria (Bacteroidetes, Firmicutes, Verrucomicrobia, Parabacteroides, Lactobacillus, and Akkermansia) and harmful bacteria (Proteobacteria and Enterobacter), repairing the damage induced by dual administration of antibiotics and LPS.ConclusionZG attenuates the dual induction of antibiotics and LPS-induced intestinal barrier damage and also protects the intestinal barrier function in mice.

show abstract

Section: Effects Of Zinc Gluconate On the Ultrastructure Of Mouse Ileummentioning

confidence: 99%

Protective effect of zinc gluconate on intestinal mucosal barrier injury in antibiotics and LPS-induced mice

Wang,

Xiao,

Wei

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…In contrast, CD can impact any part of the digestive system, with a particular focus on the terminal ileum. Evidence from previous studies suggests that oxidative stress plays a critical in the development of IBD (5)(6)(7). Previous Mendelian randomization (MR) analysis also identified oxidative stressrelated genetic risk loci for CD (8).…”

Section: Introductionmentioning

confidence: 99%

Diet-derived circulating antioxidants and risk of inflammatory bowel disease: a Mendelian randomization study and meta-analysis

Zou,

Liang,

Zhang

et al. 2024

Front. Immunol.

View full text Add to dashboard Cite

BackgroundPrevious studies have shown conflicting results regarding the impact of circulating antioxidants on the risk of inflammatory bowel disease (IBD). In this study, our intent was to investigate the causal relationship between circulating antioxidants and IBD using Mendelian randomization (MR).MethodsInstrumental variables for absolute circulating antioxidants (ascorbate, retinol, lycopene, and β-carotene) and circulating antioxidant metabolites (α-tocopherol, γ-tocopherol, ascorbate, and retinol) were screened from published studies. We obtained outcome data from two genome-wide association study (GWAS) databases, including the international inflammatory bowel disease genetics consortium (IIBDGC, 14,927 controls and 5,956 cases for Crohn’s disease (CD), 20,464 controls and 6,968 cases for ulcerative colitis (UC), and 21,770 controls and 12,882 cases for IBD) and the FinnGen study (375,445 controls and 1,665 cases for CD, 371,530 controls and 5,034 cases for UC, and 369,652 controls and 7,625 cases for IBD). MR analysis was performed in each of the two databases and those results were pooled using meta-analysis to assess the overall effect of exposure on each phenotype. In order to confirm the strength of the findings, we additionally conducted a replication analysis using the UK Biobank.ResultsIn the meta-analysis of the IIBDGC and FinnGen, we found that each unit increase in absolute circulating level of retinol was associated with a 72% reduction in the risk of UC (OR: 0.28, 95% CI: 0.10 to 0.78, P=0.015). The UC GWAS data from the UK Biobank also confirmed this causal relationship (OR: 0.99, 95% CI: 0.97 to 1.00, P=0.016). In addition, there was suggestive evidence that absolute retinol level was negatively associated with IBD (OR: 0.41, 95% CI: 0.18 to 0.92, P=0.031). No other causal relationship was found.ConclusionOur results provide strong evidence that the absolute circulating level of retinol is associated with a reduction in the risk of UC. Further MR studies with more instrumental variables on circulating antioxidants, especially absolute circulating antioxidants, are needed to confirm our results.

show abstract

Nanotherapeutics-mediated restoration of pancreatic homeostasis and intestinal barrier for the treatment of severe acute pancreatitis

Lin,

Wei,

et al. 2025

Journal of Controlled Release

View full text Add to dashboard Cite

Engineered baicalein-decorated zinc phosphates for synergistic alleviation of inflammatory bowel disease by repairing the mucosal barrier and relieving oxidative stress

Abstract: Orally administrating baicalein-decorated zinc phosphates (ZnBM) were engineered for mucosal barrier improvement and intestinal inflammation relief. ZnBM with a size of 1.78 µm comprised 5.58 wt% of baicalein and 13.17...

Cited by 5 publications

References 44 publications

Protective effect of zinc gluconate on intestinal mucosal barrier injury in antibiotics and LPS-induced mice

Protective effect of zinc gluconate on intestinal mucosal barrier injury in antibiotics and LPS-induced mice

Diet-derived circulating antioxidants and risk of inflammatory bowel disease: a Mendelian randomization study and meta-analysis

Nanotherapeutics-mediated restoration of pancreatic homeostasis and intestinal barrier for the treatment of severe acute pancreatitis

Contact Info

Product

Resources

About