Engineered biochemical cues of regenerative biomaterials to enhance endogenous stem/progenitor cells (ESPCs)-mediated articular cartilage repair

Zhou, Liangbin; Xu, Jietao; Schwab, Albrecht; Tong, Wenxue; Xu, Jiankun; Zheng, Lizhen; Li, Ye; Li, Zhuo; Xu, Shunxiang; Chen, Ziyi; Zou, Li; Zhao, Xin; Osch, Gerjo J.V.M. van; Wen, Chunyi; Qin, Ling

doi:10.1016/j.bioactmat.2023.03.008

Cited by 11 publications

(9 citation statements)

References 169 publications

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“…The next frontier in the advancement of ferroptosis-based cancer therapeutics lies in the effective ferroptosis induction of cancer cells with minimized side effects. An effective mean of harnessing the clinical potential of ferroptosis-mediated cancer therapy is local image guided ferroptosis- and combinational ferroptosis-cancer therapy regimen [ [205] , [206] , [207] , [208] ]. As considering combinational ferroptosis cancer therapy, the rational design of the combination for the synergistic anticancer effects is of significant importance [ [209] , [210] , [211] , [212] , [213] ].…”

Section: Discussionmentioning

confidence: 99%

Magnetic nanoparticles for ferroptosis cancer therapy with diagnostic imaging

Ko,

Min,

Hong

et al. 2024

Bioactive Materials

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Section: Discussionmentioning

confidence: 99%

Magnetic nanoparticles for ferroptosis cancer therapy with diagnostic imaging

Ko,

Min,

Hong

et al. 2024

Bioactive Materials

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“…Seed cells, scaffolds and cytokines are the three main factors for tissue engineering repair. Previous studies have shown that endogenous stem cells exist in almost all adult tissues and organs and that these stem cells have strong potential in repair, regeneration and homeostasis maintenance after tissue injury (Zhou et al 2023 ). An increasing number of studies have also confirmed the presence of stem cells in intervertebral disc tissue; these cells originate from different anatomical regions of the intervertebral disc, such as endplate cartilage, the annulus fibrosus, and the nucleus pulposus (Zhang et al 2021 ; Luo et al 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Decellularized nucleus pulposus matrix/chitosan hybrid hydrogel combined with nucleus pulposus stem cells and GDF5-loaded microspheres for intervertebral disc degeneration prevention

Ma,

Liu,

Zhao

et al. 2024

Mol Med

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Background Intervertebral disc degeneration (IDD) is considered an important pathological basis for spinal degenerative diseases. Tissue engineering is a powerful therapeutic strategy that can effectively restore the normal biological properties of disc units. In this study, hydrogels loaded with growth/differentiation factor 5 (GDF5) and stem cells were combined to provide an effective strategy for nucleus pulposus regeneration. Methods Nucleus pulposus stem cells (NPSCs) were obtained by low-density inoculation and culture, and their stem cell characteristics were verified by flow cytometry and a tri-lineage-induced differentiation experiment. A decellularized nucleus pulposus matrix (DNPM) and chitosan hybrid hydrogel was prepared, and GDF5-loaded poly(lactic-co-glycolic acid) (PLGA) microspheres were incorporated into the hydrogels to obtain a composite hydrogels with GDF5-loaded microspheres. Taking bone marrow mesenchymal stem cells (BMSCs) as a reference, the effect of composite hydrogels with GDF5-loaded microspheres on the chondrogenic differentiation of NPSCs was evaluated. A model of intervertebral disc degeneration induced by acupuncture on the tail of rats was constructed, and the repair effect of composite hydrogels with GDF5-loaded microspheres combined with NPSCs on IDD was observed. Results Stem cell phenotype identification, stemness gene expression and tri-lineage-induced differentiation confirmed that NPSCs had characteristics similar to those of BMSCs. The rat DNPM and chitosan hybrid hydrogels had good mechanical properties, and the GDF5-loaded microspheres sustainably released GDF5. NPSCs grew normally in the composite hydrogels and gradually expressed a chondrocyte phenotype. Animal experiments showed that the composite hydrogels with GDF5-loaded microspheres combined with NPSCs effectively promoted nucleus pulposus regeneration and that the effect of the hydrogels on the repair of IDD was significantly better than that of BMSCs. Conclusion GDF5-loaded microspheres combined with DNPM/chitosan composite hydrogels can effectively promote the differentiation of NPSCs into nucleus pulposus-like cells and effectively preventIDD.

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“…To enhance endogenous cell recruitment to the injury site, the biological process within a living organism after articular cartilage injury needs to be clarified. Considering species differences, appropriate animal models to replicate human articular cartilage repair processes are reported in mice [51][52][53], rats [54], rabbits [55][56][57], horses [58], and canines [59], thus molecular reaction after articular cartilage injuries is continuously disclosed [60][61][62][63][64]. Even though the species are different, the major ganglioside in articular cartilage appears to be GM3 [65,66].…”

Section: Endogenous Potential To Heal In Articular Cartilagementioning

confidence: 99%

Glycosphingolipids in Osteoarthritis and Cartilage Regeneration Therapy: Mechanisms and Therapeutic Prospects

Homan,

Onodera,

Matsuoka

et al. 2024

Preprint

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Glycosphingolipids (GSLs), a subtype of glycolipids containing sphingosine, are critical components of vertebrate plasma membranes, playing a pivotal role in cellular signaling and interactions. In human articular cartilage in osteoarthritis (OA), GSL expression is known notably to decrease. This review focuses on the roles of gangliosides, a specific type of GSL, in cartilage degeneration and regeneration, emphasizing their regulatory function in signal transduction. The expression of gangliosides, whether endogenous or augmented exogenously, is regulated at the enzymatic level, targeting specific glycosyltransferases. This regulation has significant implications for the composition of cell surface gangliosides and their impact on signal transduction in chondrocytes and progenitor cells. Different levels of ganglioside expression can influence signaling pathways in various ways, potentially affecting cell properties, including malignancy. Moreover, gene manipulations against gangliosides have been shown to regulate cartilage metabolism and chondrocyte differentiation in vivo and in vitro. This review highlights the potential of targeting gangliosides in the development of therapeutic strategies for osteoarthritis and cartilage injury and addresses promising directions for future research and treatment.

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Engineered biochemical cues of regenerative biomaterials to enhance endogenous stem/progenitor cells (ESPCs)-mediated articular cartilage repair

Cited by 11 publications

References 169 publications

Magnetic nanoparticles for ferroptosis cancer therapy with diagnostic imaging

Magnetic nanoparticles for ferroptosis cancer therapy with diagnostic imaging

Decellularized nucleus pulposus matrix/chitosan hybrid hydrogel combined with nucleus pulposus stem cells and GDF5-loaded microspheres for intervertebral disc degeneration prevention

Glycosphingolipids in Osteoarthritis and Cartilage Regeneration Therapy: Mechanisms and Therapeutic Prospects

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