2008
DOI: 10.3233/bir-2008-0490
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Engineered cartilage constructs subject to very low regimens of interstitial perfusion

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Cited by 31 publications
(23 citation statements)
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“…Alternatively suboptimal levels of shear stress could lead to a reduction in chondrocyte specific gene expression compared to static cultures . However, using a cylindrical pore model for the approximation of the scaffold geometry the initial levels of shear stress used in this study were estimated to be approximately 5 mPa, which was previously found to lead to higher GAG/DNA ratios than other shear levels investigated and is on the same level of shear stresses used by other investigators . Furthermore, the reduction in collagen I expression that was also observed in the perfusion cultures, compared to static, may be further evidence that the levels of shear stress were not too high, as chondrocytes have been shown to increase production of collagen type I in response to high shear .…”
Section: Resultsmentioning
confidence: 99%
“…Alternatively suboptimal levels of shear stress could lead to a reduction in chondrocyte specific gene expression compared to static cultures . However, using a cylindrical pore model for the approximation of the scaffold geometry the initial levels of shear stress used in this study were estimated to be approximately 5 mPa, which was previously found to lead to higher GAG/DNA ratios than other shear levels investigated and is on the same level of shear stresses used by other investigators . Furthermore, the reduction in collagen I expression that was also observed in the perfusion cultures, compared to static, may be further evidence that the levels of shear stress were not too high, as chondrocytes have been shown to increase production of collagen type I in response to high shear .…”
Section: Resultsmentioning
confidence: 99%
“…Active methods for improving nutrient uptake include bioreactors that apply compression 24,29,30,35,36,75,80 or rotation 102,103 and perfusion devices. 78,80,104,105 As discussed in the previous section, physiological levels of compressive dynamic loading ($10% strain, less than 6 hours) may be ideal for enhancing nutrient diffusion into engineered cartilage. 33 Future work will need to combine these techniques to understand whether the effects of increased porosity via micro-or macrochannels can be additive to the improved nutrient diffusion from dynamic loading (►Fig.…”
Section: Resultsmentioning
confidence: 99%
“…79 In contrast, studies that have combined perfusion with dynamic loading have not observed additional nutritional benefits from perfusion. 34,78,80 The findings of these studies suggest some potential benefits of using perfusion in the absence of mechanical loading stimuli to improve the compositional and mechanical properties of immature osteochondral constructs. This will be especially important for large osteochondral constructs, because the bone/substrate interface will make limited nutrient diffusion into constructs an even greater challenge to overcome.…”
Section: Cell Sources For Cartilage Tissue Engineeringmentioning
confidence: 97%
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“…To achieve tissue regeneration, cell‐based therapies, tissue‐inducing factors, and biocompatible scaffolds have been investigated singularly and in combination 2–15…”
Section: Introductionmentioning
confidence: 99%