“…An enhanced production of ethanol was observed when overexpressing selected enzymes of CO 2 fixing Calvin-Benson-Bassham cycle (Liang et al, 2018;Roussou et al, 2021). Finally, a nonfunctional hydrogenase apoprotein, origin of green algae and expressed in living cells of a unicellular cyanobacterium, was successfully activated by introducing a synthetic complex mimicking the active site.…”
Section: Photosynthetic Cell Factoriesmentioning
confidence: 99%
“…Specifically, the application of systematic modular engineering enabled the efficient biosynthesis of 1‐butanol, an attractive commodity chemical and gasoline substitute, with a cumulative titer of 4.8 g L −1 being the highest 1‐butanol yield from CO 2 reported thus far (Liu et al, 2019). An enhanced production of ethanol was observed when overexpressing selected enzymes of CO 2 fixing Calvin‐Benson‐Bassham cycle (Liang et al, 2018; Roussou et al, 2021). Finally, a non‐functional hydrogenase apoprotein, origin of green algae and expressed in living cells of a unicellular cyanobacterium, was successfully activated by introducing a synthetic complex mimicking the active site.…”
NordAqua is a multidisciplinary Nordic Center of Excellence funded by NordForsk Bioeconomy program (2017)(2018)(2019)(2020)(2021)(2022). The research center promotes Blue Bioeconomy and endeavours to reform the use of natural resources in a environmentally sustainable way. In this short communication, we summarize particular outcomes of the consortium. The key research progress of NordAqua includes (1) improving of photosynthetisis, (2) developing novel photosynthetic cell factories that function in a "solar-driven direct CO 2 capture to target bioproducts" mode, (3) promoting the diversity of Nordic cyanobacteria and algae as an abundant and resilient alternative for less sustainable forest biomass and for innovative production of biochemicals, and (4) improving the bio-based wastewater purification and nutrient recycling technologies to provide new tools for integrative circular economy platforms.A rapid replacement of carbon-intensive infrastructures with net-zero carbon alternatives is vital for mitigation of the climate crisis. Current bioeconomy approaches have been focused mainly on the replacement of fossil raw materials for bio-based commodities. To this end, Nordic countries have largely invested in wood biomass which, for a number of reasons, cannot be considered sustainable or sufficiently available to effectuate a major change. The NordAqua research center has taken a different approach and focuses on aquatic photosynthetic
“…An enhanced production of ethanol was observed when overexpressing selected enzymes of CO 2 fixing Calvin-Benson-Bassham cycle (Liang et al, 2018;Roussou et al, 2021). Finally, a nonfunctional hydrogenase apoprotein, origin of green algae and expressed in living cells of a unicellular cyanobacterium, was successfully activated by introducing a synthetic complex mimicking the active site.…”
Section: Photosynthetic Cell Factoriesmentioning
confidence: 99%
“…Specifically, the application of systematic modular engineering enabled the efficient biosynthesis of 1‐butanol, an attractive commodity chemical and gasoline substitute, with a cumulative titer of 4.8 g L −1 being the highest 1‐butanol yield from CO 2 reported thus far (Liu et al, 2019). An enhanced production of ethanol was observed when overexpressing selected enzymes of CO 2 fixing Calvin‐Benson‐Bassham cycle (Liang et al, 2018; Roussou et al, 2021). Finally, a non‐functional hydrogenase apoprotein, origin of green algae and expressed in living cells of a unicellular cyanobacterium, was successfully activated by introducing a synthetic complex mimicking the active site.…”
NordAqua is a multidisciplinary Nordic Center of Excellence funded by NordForsk Bioeconomy program (2017)(2018)(2019)(2020)(2021)(2022). The research center promotes Blue Bioeconomy and endeavours to reform the use of natural resources in a environmentally sustainable way. In this short communication, we summarize particular outcomes of the consortium. The key research progress of NordAqua includes (1) improving of photosynthetisis, (2) developing novel photosynthetic cell factories that function in a "solar-driven direct CO 2 capture to target bioproducts" mode, (3) promoting the diversity of Nordic cyanobacteria and algae as an abundant and resilient alternative for less sustainable forest biomass and for innovative production of biochemicals, and (4) improving the bio-based wastewater purification and nutrient recycling technologies to provide new tools for integrative circular economy platforms.A rapid replacement of carbon-intensive infrastructures with net-zero carbon alternatives is vital for mitigation of the climate crisis. Current bioeconomy approaches have been focused mainly on the replacement of fossil raw materials for bio-based commodities. To this end, Nordic countries have largely invested in wood biomass which, for a number of reasons, cannot be considered sustainable or sufficiently available to effectuate a major change. The NordAqua research center has taken a different approach and focuses on aquatic photosynthetic
Section: Modulating Flux Through Pyruvatementioning
confidence: 99%
“…In addition, carbon fixation rates increased, with total biomass production (dry cell weight and ethanol) increasing by 7.7%, 15.1%, 8.8% and 10.1%, respectively [ 78 ]. The tandem overexpression of fba and tkl yielded nine-fold and four-fold higher ethanol production than solely overexpressing fba or tkl, respectively [ 31 ]. However, the best performing fba/tkl strain only produced 1.2 g/L ethanol after 20 days [ 31 ], far below best performing Synechocystis strain after 26 days (5.50 gL −1 ethanol; [ 24 ]).…”
Section: Modulating Flux Through Pyruvatementioning
confidence: 99%
“…The tandem overexpression of fba and tkl yielded nine-fold and four-fold higher ethanol production than solely overexpressing fba or tkl, respectively [ 31 ]. However, the best performing fba/tkl strain only produced 1.2 g/L ethanol after 20 days [ 31 ], far below best performing Synechocystis strain after 26 days (5.50 gL −1 ethanol; [ 24 ]). These studies suggest overexpressing key enzymes involved in pyruvate production could improve overall flux towards ethanol production [ 31 ].…”
Section: Modulating Flux Through Pyruvatementioning
Current industrial bioethanol production by yeast through fermentation generates carbon dioxide. Carbon neutral bioethanol production by cyanobacteria uses biological fixation (photosynthesis) of carbon dioxide or other waste inorganic carbon sources, whilst being sustainable and renewable. The first ethanologenic cyanobacterial process was developed over two decades ago using Synechococcus elongatus PCC 7942, by incorporating the recombinant pdc and adh genes from Zymomonas mobilis. Further engineering has increased bioethanol titres 24-fold, yet current levels are far below what is required for industrial application. At the heart of the problem is that the rate of carbon fixation cannot be drastically accelerated and carbon partitioning towards bioethanol production impacts on cell fitness. Key progress has been achieved by increasing the precursor pyruvate levels intracellularly, upregulating synthetic genes and knocking out pathways competing for pyruvate. Studies have shown that cyanobacteria accumulate high proportions of carbon reserves that are mobilised under specific environmental stresses or through pathway engineering to increase ethanol production. When used in conjunction with specific genetic knockouts, they supply significantly more carbon for ethanol production. This review will discuss the progress in generating ethanologenic cyanobacteria through chassis engineering, and exploring the impact of environmental stresses on increasing carbon flux towards ethanol production.
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