Engineered Extracellular Vesicles: A potential treatment for regeneration

Cheng, Wen; Xu, Chenyu; Su, Yuran; Shen, Youqing; Yang, Qiang; Zhao, Yanmei; Zhao, Yanhong; Liu, Yue

doi:10.1016/j.isci.2023.108282

Cited by 8 publications

(2 citation statements)

References 132 publications

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“… 119 Within this context, to overcome some limitations of EVs such as inefficient cell targeting, on demand delivery, and low yield, EV can be engineered by modifying the donor cells (e.g., stress induction or 3D cell culture) or by a direct modification of the EVs (e.g., a direct loading or membrane surface strategies). 120 Efforts are already being made in deciphering the mechanisms underlying targeted EV uptake, through which the delivery of functional EV-encapsulated biomolecules to recipient cells occurs 74 ; superiorly robust EV detection methods will also contribute to the establishment of EV usage in clinical contexts. 121 …”

Section: Concluding Remarks and Future Perspectivesmentioning

confidence: 99%

Exploring the role of epicardial adipose-tissue-derived extracellular vesicles in cardiovascular diseases

Rizzuto,

Gelpi,

Mangini

et al. 2024

iScience

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Section: Concluding Remarks and Future Perspectivesmentioning

confidence: 99%

Exploring the role of epicardial adipose-tissue-derived extracellular vesicles in cardiovascular diseases

Rizzuto,

Gelpi,

Mangini

et al. 2024

iScience

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“…Engineering strategies to optimize EVs for therapeutic purposes include surface modification, loading specific therapeutic agents, and genetic engineering. The application of engineered EVs in regenerative medicine has recently been summarized, and two categories of engineered EVs have been marked: those produced by donor cells and directly engineered EVs [7,63].…”

Section: Modification Of Sevs For Targeted Pain Managementmentioning

confidence: 99%

Contribution of Small Extracellular Vesicles from Schwann Cells and Satellite Glial Cells to Pain Processing

Gazerani

2024

Neuroglia

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Extracellular vesicles (EVs), including exosomes and microvesicles, are membrane-bound particles released by cells into extracellular space. These vesicles carry various molecules, such as proteins and lipids, and can serve as mediators of intercellular communication. EVs have been implicated in the communication between different cell types in the nervous system, for instance, the neurons and glial cells of the central nervous system (CNS) and peripheral nervous system (PNS). Satellite glial cells (SGCs) surround and support neurons in the sensory ganglia of the PNS, and it has been proposed that the EVs released by SGCs may contribute to the processing of pain-related signals and features. This includes the modulation of neuronal activity, the release of pro-inflammatory signaling molecules, and sensitization. A noticeable finding is that EVs can transfer bioactive molecules, including proteins and microRNAs (miRNAs), between cells, influencing cellular functions such as gene expression regulation involved in the transmission and modulation of pain signals. Schwann cells (SCs) also release EVs. SC-derived EVs sequester TNFR1, influencing TNFα activity and regulating neuroinflammation in peripheral nerve injuries. Understanding peripheral glia’s EVs role in pain processing is an emerging area in neuroscience. Here, the latest findings, challenges, and potential are presented to encourage future research.

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Exosomes from Hypoxia Preconditioned Muscle‐Derived Stem Cells Enhance Cell‐Free Corpus Cavernosa Angiogenesis and Reproductive Function Recovery

Peng,

Chai,

Chen

et al. 2024

Adv Healthcare Materials

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Tissue engineering for penile corpora cavernosa defects requires microvascular system reconstruction.GelMA hydrogels show promise for tissue regeneration. However, using stem cells faces challenges such as immune rejection, limited proliferation and differentiation, and biosafety concerns. Therefore, acellular tissue regeneration may avoid these issues. Exosomes are used from muscle‐derived stem cells (MDSCs) to modify 3D‐printed hydrogel scaffolds for acellular tissue regeneration. Hypoxia‐preconditioned MDSC‐derived exosomes are obtained to enhance the therapeutic effect. In contrast to normoxic exosomes (N‐Exos), hypoxic exosomes (H‐Exos) are found to markedly enhance the proliferation, migration, and capillary‐like tube formation of human umbilical vein endothelial cells (HUVECs). High‐throughput sequencing analysis of miRNAs isolated from both N‐Exos and H‐Exos revealed a significant upregulation of miR‐21‐5p in H‐Exos following hypoxic preconditioning. Further validation demonstrated that the miR‐21‐5p/PDCD4 pathway promoted the proliferation of HUVECs. Epigallocatechin gallate (EGCG) is introduced to improve the mechanical properties and biocompatibility of GelMA hydrogels. EGCG‐GelMA scaffolds loaded with different types of Exos are transplanted to repair rabbit penile corpora cavernosa defects, observed the blood flow and repair status of the defect site through color Doppler ultrasound and magnetic resonance imaging, and ultimately restored the rabbit penile erection function and successfully bred offspring. Thus, acellular hydrogel scaffolds offer an effective treatment for penile corpora cavernosa defects.

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Engineered Extracellular Vesicles: A potential treatment for regeneration

Cited by 8 publications

References 132 publications

Exploring the role of epicardial adipose-tissue-derived extracellular vesicles in cardiovascular diseases

Exploring the role of epicardial adipose-tissue-derived extracellular vesicles in cardiovascular diseases

Contribution of Small Extracellular Vesicles from Schwann Cells and Satellite Glial Cells to Pain Processing

Exosomes from Hypoxia Preconditioned Muscle‐Derived Stem Cells Enhance Cell‐Free Corpus Cavernosa Angiogenesis and Reproductive Function Recovery

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