Engineered extracellular vesicles with synthetic lipids via membrane fusion to establish efficient gene delivery

Jhan, Yong Yu; Prasca-Chamorro, Daniel; Zuniga, Guillermo Palou; Moore, David Mitchell; Kumar, Sunil; Gaharwar, Akhilesh K.; Bishop, Corey J.

doi:10.1016/j.ijpharm.2019.118802

Cited by 104 publications

(99 citation statements)

References 56 publications

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“…This approach has been applied to drugs [ 117 ] though it has mainly been used to RNA incorporation into vesicles. [ 118–120 ] This process also leads to exosomes slightly larger than the initial ones. [ 117 ] Although this method may cause RNA aggregation it has been used in clinical protocols.…”

Section: Exosomes As Drug Delivery Systemsmentioning

confidence: 99%

Advances in Exosomes‐Based Drug Delivery Systems

Gutiérrez-Millán

Díaz

Lanao

et al. 2020

Macromolecular Bioscience

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Exosomes, a subgroup of extracellular vesicles, are important mediators of long‐distance intercellular communication and are involved in a diverse range of biological processes such as the transport of lipids, proteins, and nucleic acids. Researchers, seeing the problems caused by the toxic effects and clearance of synthetic nanoparticles, consider exosomes as an interesting alternative to such nanoparticles in the specific and controlled transport of drugs. In recent years, there have been remarkable advances in the use of exosomes in cancer therapeutics or for treating neurological diseases, among other applications. The objective of this work is to analyze studies focused on exosomes used in drug delivery system, present and future applications in this field of research are discussed based on the results obtained.

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Section: Exosomes As Drug Delivery Systemsmentioning

confidence: 99%

Advances in Exosomes‐Based Drug Delivery Systems

Gutiérrez-Millán

Díaz

Lanao

et al. 2020

Macromolecular Bioscience

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show abstract

“…Jhan et al fused several lipids such as DOTAP, POPC, DPPC, and POPG with EVs to make a hybrid-lipid membrane structure and then loaded siRNA into EVs through electroporation. These engineered EVs showed a higher gene silencing effect than lipofectamine transfection [136]. Jeyaram et al loaded GAPDH siRNA through pH gradient modification of EVs.…”

Section: Sirnamentioning

confidence: 99%

Overview and Update on Methods for Cargo Loading into Extracellular Vesicles

et al. 2021

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The enormous library of pharmaceutical compounds presents endless research avenues. However, several factors limit the therapeutic potential of these drugs, such as drug resistance, stability, off-target toxicity, and inadequate delivery to the site of action. Extracellular vesicles (EVs) are lipid bilayer-delimited particles and are naturally released from cells. Growing evidence shows that EVs have great potential to serve as effective drug carriers. Since EVs can not only transfer biological information, but also effectively deliver hydrophobic drugs into cells, the application of EVs as a novel drug delivery system has attracted considerable scientific interest. Recently, EVs loaded with siRNA, miRNA, mRNA, CRISPR/Cas9, proteins, or therapeutic drugs show improved delivery efficiency and drug effect. In this review, we summarize the methods used for the cargo loading into EVs, including siRNA, miRNA, mRNA, CRISPR/Cas9, proteins, and therapeutic drugs. Furthermore, we also include the recent advance in engineered EVs for drug delivery. Finally, both advantages and challenges of EVs as a new drug delivery system are discussed. Here, we encourage researchers to further develop convenient and reliable loading methods for the potential clinical applications of EVs as drug carriers in the future.

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“…Since EVs have potential to deliver therapeutic agents, such as DNA, mRNA, miRNA, or peptide sequences, exclusive bioengineering of EXs [62,63] containing genes like miR-330-5p, miR-675, miRNA -3196, or miRNA-203 could offer a therapeutic approach for hypo-and hyperpigmentation disorders by regulating the balance of expression of melanocyte proteins.…”

Section: Cross-talk Between Keratinocytes and Melanocytes Through Evsmentioning

confidence: 99%

Shedding light on the role of keratinocyte-derived extracellular vesicles on skin-homing cells

et al. 2020

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Extracellular vesicles (EVs) are secretory lipid membranes with the ability to regulate cellular functions by exchanging biological components between different cells. Resident skin cells such as keratinocytes, fibroblasts, melanocytes, and inflammatory cells can secrete different types of EVs depending on their biological state. These vesicles can influence the physiological properties and pathological processes of skin, such as pigmentation, cutaneous immunity, and wound healing. Since keratinocytes constitute the majority of skin cells, secreted EVs from these cells may alter the pathophysiological behavior of other skin cells. This paper reviews the contents of keratinocyte-derived EVs and their impact on fibroblasts, melanocytes, and immune cells to provide an insight for better understanding of the pathophysiological mechanisms of skin disorders and their use in related therapeutic approaches.

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Engineered extracellular vesicles with synthetic lipids via membrane fusion to establish efficient gene delivery

Cited by 104 publications

References 56 publications

Advances in Exosomes‐Based Drug Delivery Systems

Advances in Exosomes‐Based Drug Delivery Systems

Overview and Update on Methods for Cargo Loading into Extracellular Vesicles

Shedding light on the role of keratinocyte-derived extracellular vesicles on skin-homing cells

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