Engineered GH11 xylanases from <i>Orpinomyces</i> sp. PC‐2 improve techno‐functional properties of bread dough

Passarinho, Amanda Tafuri Paniago; Ventorim, Rafaela Zandonade; Maitan-Alfenas, Gabriela Píccolo; Oliveira, Eduardo Basílio de; Guimarães, Valéria Monteze

doi:10.1002/jsfa.9242

Cited by 16 publications

(4 citation statements)

References 28 publications

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“…In recent studies, Mesoflavibacter zeaxanthinifaciens was used to extract glutaminase-free ʟ-asparaginase enzyme that was involved in asparagine hydrolysis and played a significant role in cancer cells (Lee et al, 2016). Another study also showed its employment in the extraction of the xylanase enzyme (Oh et al, 2011) that is highly demanding in the bakery industry (Passarinho et al, 2018). Mesoflavibacter zeaxanthinifaciens is one of the essential bacteria that produce antibacterial compound during rapid and efficient screening method (Zhao et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Silver Nanomaterial Synthesis From Mesoflavibacter zeaxanthinifaciens and Targeting Biofilm Formation

et al. 2019

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Considering the significance of biological and eco-friendly nanomaterials, in the present study, we have synthesized silver nanoparticles from the exopolysaccharide of recently recovered bacterial strain CEES51 from the Red Sea coastal area of Jeddah, Saudi Arabia. 16S ribosomal RNA gene sequencing was used to characterize the isolated bacteria, and it was identified as Mesoflavibacter zeaxanthinifaciens and assigned an accession number MH707257.1 GenBank. The bacterial strain is an excellent exopolysaccharide producer and survived at hypersaline (30%) and high-temperature (50°C) conditions. The bacterial exopolysaccharides were employed for the fabrication of silver nanoparticles at room temperature. UV-visible spectrophotometer optimized the synthesized nanoparticles, and their size was determined by Nanophox particle size analyzer and dynamic light scattering. Additionally, the X-ray powder diffraction and Fourier-transform infrared spectroscopy studies also approved its crystalline nature and the involvement of organic functional groups in their formation. The synthesized nanomaterials were tested for their antibacterial and antibiofilm properties against pathogenic microorganisms Bacillus subtilis and methicillin-resistant Staphylococcus aureus . The antimicrobial property showed time, and dose-dependent response with a maximum of zone inhibition was observed at around 22 and 18 mm at a dose of 50 µg/well against B. subtilis and S. aureus and a minimum inhibitory concentration of 8 and 10 µg/ml, respectively. Furthermore, the synthesized silver nanoparticles possessed a substantial antibiofilm property and were also found to be biocompatible as depicted by red blood cell lysis assay and their interaction with peripheral blood mononuclear cells and human embryonic kidney 293 cells. Therefore, Mesoflavibacter zeaxanthinifaciens is found to be an excellent source for exopolysaccharide synthesis that assists in the silver nanoparticle production.

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Section: Introductionmentioning

confidence: 99%

Antibacterial Silver Nanomaterial Synthesis From Mesoflavibacter zeaxanthinifaciens and Targeting Biofilm Formation

et al. 2019

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“…In studies on the characterization of Orpinomyces sp, its properties such as acetyl xylan esterase enzyme, optimum pH, temperature and molecular weight were determined (Blumm et al, 1999;Razeq et al, 2011).The enzyme xylanase is actively used in the production of biofuels and different industrial fields, and Orpinomyces sp. studies on the production of thermo-stable form have been reported (Passarinho et al,2019;Ventorim et al, 2018). 1.4.3.…”

Section: Carbohydrate Active Enzymes System In Neocallimastix Spmentioning

confidence: 99%

Carbohydrate active enzyme system in rumen fungi: a review

YÜCEL

EKİNCİ

2022

International Journal of Secondary Metabolite

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Hydrolysis and dehydration reactions of carbohydrates, which are used as energy raw materials by all living things in nature, are controlled by Carbohydrate Active Enzyme (CAZy) systems. These enzymes are also used in different industrial areas today. There are different types of microorganisms that have the CAZy system and are used in the industrial sector. Apart from current organisms, there are also rumen fungi within the group of candidate microorganisms with the CAZy system. It has been reported that xylanase (EC3.2.1.8 and EC3.2.1.37) enzyme, a member of the glycoside hydrolase enzyme family obtained from Trichoderma sp. and used especially in areas such as bread, paper, and feed industry, is more synthesized in rumen fungi such as Orpinomyces sp. and Neocallimastix sp. Therefore, this study reviews Neocallimastixsp., Orpinomyces sp., Caecomyces sp., Piromyces sp., and Anaeromyces sp., registered in the CAZy and Mycocosm database for rumen fungi to have both CAZy enzyme activity and to be an alternative microorganism in the industry. Furthermore the CAZy enzyme activities of the strains are investigated. The review shows thatNeocallimax sp. and Orpinomyces sp. areconsidered as candidate microorganisms.

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“…Xylanase treatment improves the quality of bread as it reduces the staling rate and increases the shelf-life ( Liu et al, 2017 ; Passarinho et al, 2019 ). Staling causes significant financial loss to the baking industry.…”

Section: Applications Of Extremophilic Xylanasesmentioning

confidence: 99%

Extremophilic Prokaryotic Endoxylanases: Diversity, Applicability, and Molecular Insights

Verma

2021

Front. Microbiol.

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Extremophilic endoxylanases grabbed attention in recent years due to their applicability under harsh conditions of several industrial processes. Thermophilic, alkaliphilic, and acidophilic endoxylanases found their employability in bio-bleaching of paper pulp, bioconversion of lignocellulosic biomass into xylooligosaccharides, bioethanol production, and improving the nutritious value of bread and other bakery products. Xylanases obtained from extremophilic bacteria and archaea are considered better than fungal sources for several reasons. For example, enzymatic activity under broad pH and temperature range, low molecular weight, cellulase-free activity, and longer stability under extreme conditions of prokaryotic derived xylanases make them a good choice. In addition, a short life span, easy cultivation/harvesting methods, higher yield, and rapid DNA manipulations of bacterial and archaeal cells further reduces the overall cost of the product. This review focuses on the diversity of prokaryotic endoxylanases, their characteristics, and their functional attributes. Besides, the molecular mechanisms of their extreme behavior have also been presented here.

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Engineered GH11 xylanases from Orpinomyces sp. PC‐2 improve techno‐functional properties of bread dough

Abstract: The recombinant xylanases showed potential for application in bakery processing and can improve techno-functional properties in sponges. © 2018 Society of Chemical Industry.

Cited by 16 publications

References 28 publications

Antibacterial Silver Nanomaterial Synthesis From Mesoflavibacter zeaxanthinifaciens and Targeting Biofilm Formation

Antibacterial Silver Nanomaterial Synthesis From Mesoflavibacter zeaxanthinifaciens and Targeting Biofilm Formation

Carbohydrate active enzyme system in rumen fungi: a review

Extremophilic Prokaryotic Endoxylanases: Diversity, Applicability, and Molecular Insights

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