Engineered glyphosate oxidase coupled to spore-based chemiluminescence system for glyphosate detection

Qin, Yuqing; Wu, Gaobing; Guo, Yiming; Ke, Da; Yin, Jiakang; Wang, Donglin; Fan, Xuezhu; Liu, Ziduo; Ruan, Lifang; Hu, Yonggang

doi:10.1016/j.aca.2020.07.077

Cited by 24 publications

(10 citation statements)

References 48 publications

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“…There has been much published work whose detection modes are based on UV–vis spectrophotometry. For data analysis or method evaluation, the concentration data corresponding to the absorbance values can be used in the form of linear or logarithmic plots. − Concentration data when enabled in an acceptable linear way is simple. However, if there is unsatisfactory linearity, there is a need for invoking different mathematical functions for curve fitting.…”

Section: Common Applications With Logarithm Of Concentrationmentioning

confidence: 99%

Logarithmic Data Processing Can Be Used Justifiably in the Plotting of a Calibration Curve

et al. 2021

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The article is a response to a recent opinion piece that log concentration values should not be applied in analytical chemistry. An essential aim in the development of analytical chemistry methods is to obtain more sensitive and accurate detection values. For the application of chemical analysis methods, the obtained experiment data need to fit with the mathematical functions in the first place. As influenced by different detection principles and analytical methods, data can be displayed in a coordinate system with two linear axes for linear function fitting, or the data can first be taken through a logarithmic transformation and then for function fitting. Using raw data or data after logarithmic transformation primarily depends on analytical principles, without special rules of data formats. For example, ultraviolet− visible spectrophotometric data are more suitable for direct linear fitting. However, enzyme-catalyzed reaction or electrochemical data in logarithmic form are more appropriate for function fitting. This transformation of data form will not affect the soundness of fit statistics; rather, it simplifies the complexity of function analysis and calculation, which are the essence of analytical chemistry. In this brief article, we provide justification and legitimacy of the application of logarithmic processing in various fields of quantitative analytical chemistry.

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Section: Common Applications With Logarithm Of Concentrationmentioning

confidence: 99%

Logarithmic Data Processing Can Be Used Justifiably in the Plotting of a Calibration Curve

et al. 2021

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“…Zhang et al (2016) cloned glycine oxidase from Bacillus licheniformis and improved the catalytic activity of glyphosate through error-prone PCR and two rounds of DNA shu ing. In addition, the glyphosate oxidase is obtained through directed evolution of glycine oxidase and coupled with spore-luminol, which improves the sensitivity and high selectivity of glyphosate detection (Qin et al 2020). These studies have shown the degradation e ciency of the GO gene to glyphosate.In order to make the GO gene more resistant to glyphosate in plants, we chemically synthesized the GO gene from Bacillus licheniformis.…”

Section: Discussionmentioning

confidence: 99%

Functional characterization of glycine oxidase from Bacillus licheniformis in Escherichia coli and transgenic plants

Han

Zhang²,

Liang

et al. 2023

Biotechnol Lett

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ObjectivesTo nd glycine oxidase genes that can be applied to the breeding of glyphosate resistant crops. ResultsThe GO gene was chemically synthesized and transformed into glyphosate-sensitive Escherichia. coli (E.coli). The GO gene was transformed into Arabidopsis and Rice through Agrobacterium-mediated transformation. The test results con rmed that transgenic plants containing GO genes are more resistant to glyphosate than wild-type plants. On solid MS medium containing 200µM glyphosate, transgenic Arabidopsis thaliana grew normally, while wild-type plants were stunted and root growth was restricted. In a solution containing 500 µM glyphosate, wild-type Rice showed severe yellowing, while transgenic Rice grew normally. In addition, when sprayed with 10mM glyphosate solution, wild-type rice withered and died, while transgenic rice grew well. The function of GO gene to glyphosate resistance and the application value of GO gene in the cultivation of glyphosate resistant crops are proved. ConclusionsThe glycine oxidase gene from Bacillus licheniformis enhances the resistance of E. coli, Arabidopsis and Rice to glyphosate.

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“…Bacillus subtilis Detection of glyphosate; CotA laccase effectively catalyze the luminol-H 2 O 2 reaction, creating a chemiluminescent (CL) signal [175] T. versicolor The chemiluminescent (CL) immunoassay for the detection of Escherichia coli O157:H7 in synthetic samples (spring water, apple juice, skimmed milk) [176] Laccase T. versicolor immobilized on an electrospun zein fiber (ceZL)…”

Section: Biosensormentioning

confidence: 99%

Biochemical Characteristics of Laccases and their Practical Application in the Removal of Xenobiotics from Waters

Gałązka¹,

Jankiewicz²,

Szczepkowski³

2023

Preprint

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Industrialization, intensive farming, rapid population growth and urbanization are the source of a large number of pollutants entering the environment. The current concentration of xenobiotics released into the environment exceeds its natural ability to decompose them. Enzymatic degradation of pollutants seems to be an environmentally friendly process. Due to the wide spectrum of substrate specificity, from inorganic compounds to high molecular weight organic compounds such as PAH or dyes, as well as favorable biochemical properties, laccase has been used in the biological removal of xenobiotics from the environment. It is important to understand the degradation mechanisms of pollutants and to evaluate the final products in terms of their toxicity. The laccase oxidizes the substrates with the simultaneous reduction of molecular oxygen to water, which is the purest reaction co-substrate. That is why it is called a green biocatalyst. The trend is an increase in the production of enzymes related to the intensive development of industry, bioremediation or synthetic chemistry. This leads to the search for laccases with greater activity and stability under extreme conditions. The potential of laccases to degrade xenobiotics can be promoted by improving enzymatic catalytic characterization using protein engineering and other genetic engineering methods.

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Engineered glyphosate oxidase coupled to spore-based chemiluminescence system for glyphosate detection

Cited by 24 publications

References 48 publications

Logarithmic Data Processing Can Be Used Justifiably in the Plotting of a Calibration Curve

Logarithmic Data Processing Can Be Used Justifiably in the Plotting of a Calibration Curve

Functional characterization of glycine oxidase from Bacillus licheniformis in Escherichia coli and transgenic plants

Biochemical Characteristics of Laccases and their Practical Application in the Removal of Xenobiotics from Waters

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