Engineered human H-chain ferritin with reversed charge of the internal cavity exhibits RNA-mediated spongelike effect for loading RNA/DNA-binding molecules

Charousova, Marketa; Kudlickova Peskova, Marie; Takacsova, Paulina; Kapolkova, Katerina; Haddad, Yazan; Bilek, Jan; Sivak, Ladislav; Bartejs, Tomas; Heger, Zbynek; Pekarik, Vladimir

doi:10.1039/d3bm01257c

Biomater. Sci.

2024

DOI: 10.1039/d3bm01257c

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Engineered human H-chain ferritin with reversed charge of the internal cavity exhibits RNA-mediated spongelike effect for loading RNA/DNA-binding molecules

Marketa Charousova,

Marie Kudlickova Peskova,

Paulina Takacsova

et al.

Abstract: Ferritins are globular proteins with an internal cavity that enables encapsulation of a plethora of low-mass compounds. Unfortunately, the overall negative surface charge of the ferritin internal cavity hampers efficient...

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Targeted Delivery of BCL-2 siRNA Using Modified Ferritin Nanocarriers for Antitumor Therapy

Qin,

Feng,

Sun

et al. 2024

ACS Appl. Nano Mater.

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In recent years, gene therapy has emerged as a promising strategy for combating tumors. However, the inherent instability and lack of specificity of gene delivery systems pose significant challenges. To address these limitations, we propose the development of a stable, efficient, and low-toxicity delivery nanoplatform with specific targeting capabilities. Here, we utilize the ferritin cage, known for its inherent ability to target transferrin receptor 1 (TfR1), as a nanocarrier for gene delivery. Through genetic engineering and chemical coupling methods, we modified the surface of ferritin with a cell-penetrating peptide (L17E) to enhance its cellular entry, resulting in the creation of Fn-L17E. This nanoplatform demonstrates improved physiological stability compared to wild-type ferritin (Fn). Furthermore, Fn-L17E loaded with BCL-2 siRNA exhibits enhanced silencing efficiency against BCL-2, a crucial antiapoptotic protein, in both A549 and HeLa cells, indicating its potential as an effective targeted gene delivery system for antitumor therapy.

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Targeted Delivery of BCL-2 siRNA Using Modified Ferritin Nanocarriers for Antitumor Therapy

Qin,

Feng,

Sun

et al. 2024

ACS Appl. Nano Mater.

View full text Add to dashboard Cite

show abstract

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Engineered human H-chain ferritin with reversed charge of the internal cavity exhibits RNA-mediated spongelike effect for loading RNA/DNA-binding molecules

Abstract: Ferritins are globular proteins with an internal cavity that enables encapsulation of a plethora of low-mass compounds. Unfortunately, the overall negative surface charge of the ferritin internal cavity hampers efficient...

Cited by 1 publication

References 49 publications

Targeted Delivery of BCL-2 siRNA Using Modified Ferritin Nanocarriers for Antitumor Therapy

Targeted Delivery of BCL-2 siRNA Using Modified Ferritin Nanocarriers for Antitumor Therapy

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