Engineered <i>Escherichia coli</i> strains as platforms for biological production of isoprene

Lee, Hyeok Won; Park, Jung‐Ho; Kim, Won-kyo; Lee, Jin Gyeom; Lee, Ju Seok; Ahn, Jungoh; Lee, Eun Gyo; Lee, Hongweon

doi:10.1002/2211-5463.12829

Cited by 9 publications

(2 citation statements)

References 24 publications

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“…Such crops not only would have better energy utilization efficiency, reduced carbon loss, and increased yields but would also help to reduce greenhouse gas production as global warming increases. Engineering of isoprene synthesis has been reported in Cyanobacteria ( Chaves and Melis, 2018 ), Escherichia coli ( Liu et al, 2019 ; Lee et al, 2020 ), and Saccharomyces cerevisiae ( Lv et al, 2016 ) but not in field crops.…”

Section: Can the Engineering Of Crops With High Energy Utilization Efficiency Reduce Yield Losses Caused By High Temperature?mentioning

confidence: 99%

Respiration, Rather Than Photosynthesis, Determines Rice Yield Loss Under Moderate High-Temperature Conditions

Chen

Feng

et al. 2021

Front. Plant Sci.

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Photosynthesis is an important biophysical and biochemical reaction that provides food and oxygen to maintain aerobic life on earth. Recently, increasing photosynthesis has been revisited as an approach for reducing rice yield losses caused by high temperatures. We found that moderate high temperature causes less damage to photosynthesis but significantly increases respiration. In this case, the energy production efficiency is enhanced, but most of this energy is allocated to maintenance respiration, resulting in an overall decrease in the energy utilization efficiency. In this perspective, respiration, rather than photosynthesis, may be the primary contributor to yield losses in a high-temperature climate. Indeed, the dry matter weight and yield could be enhanced if the energy was mainly allocated to the growth respiration. Therefore, we proposed that engineering smart rice cultivars with a highly efficient system of energy production, allocation, and utilization could effectively solve the world food crisis under high-temperature conditions.

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Section: Can the Engineering Of Crops With High Energy Utilization Efficiency Reduce Yield Losses Caused By High Temperature?mentioning

confidence: 99%

Respiration, Rather Than Photosynthesis, Determines Rice Yield Loss Under Moderate High-Temperature Conditions

Chen

Feng

et al. 2021

Front. Plant Sci.

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“…The apparent physical properties of isoprene (C 5 H 8 , 2-methyl-1, 3-butadiene) are colorless and highly volatile at room 25 • C [24]. Isoprene found at very low concentrations in nature and is utilized as carbon source by several organisms eukaryotic and prokaryotic [25,26]. Isoprene is an important platform chemical essentially important for a production of chemicals such as synthetic rubber, sensitive adhesives, and elastomers.…”

Section: Introductionmentioning

confidence: 99%

Paper Mill Sludge as a Source of Sugars for Use in the Production of Bioethanol and Isoprene

et al. 2020

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Paper mill sludge (PMS) solids are predominantly comprised of cellulosic fibers and fillers rejected during the pulping or paper making process. Most sludges are dewatered and discharged into landfills or land spread at a cost to the mill; creating large economic and environmental burdens. This lignocellulosic residual stream can be used as a source of sugars for microbial fermentation to renewable chemicals. The aim of this study was to determine the possibility of converting mill sludge to sugars and then fermentation to either isoprene or ethanol. Chemical analysis indicated that the cellulosic fiber composition between 28 to 68% and hemicellulose content ranged from 8.4 to 10.7%. Calcium carbonate concentration in the sludge ranged from 0.4 to 34%. Sludge samples were enzyme hydrolyzed to convert cellulose fibers to glucose, percent conversion ranged from 10.5 to 98%. Calcium carbonate present with the sludge resulted in low hydrolysis rates; washing of sludge with hydrochloric acid to neutralize the calcium carbonate, increased hydrolysis rates by 50 to 88%. The production of isoprene “very low” (190 to 470 nmol) because the isoprene yields were little. Using an industrial yeast strain for fermentation of the sludge sugars obtained from all sludge samples, the maximum conversion efficiency was achieved with productivity ranging from 0.18 to 1.64 g L−1 h−1. Our data demonstrates that PMS can be converted into sugars that can be fermented to renewable chemicals for industry.

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MICROBIAL isoprene production: an overview

Isar

Jain

Joshi

et al. 2022

World J Microbiol Biotechnol

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Engineered Escherichia coli strains as platforms for biological production of isoprene

Cited by 9 publications

References 24 publications

Respiration, Rather Than Photosynthesis, Determines Rice Yield Loss Under Moderate High-Temperature Conditions

Respiration, Rather Than Photosynthesis, Determines Rice Yield Loss Under Moderate High-Temperature Conditions

Paper Mill Sludge as a Source of Sugars for Use in the Production of Bioethanol and Isoprene

MICROBIAL isoprene production: an overview

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