2018
DOI: 10.1016/j.ebiom.2018.06.005
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Engineered Kidney Tubules for Modeling Patient-Specific Diseases and Drug Discovery

Abstract: The lack of engineering systems able to faithfully reproduce complex kidney structures in vitro has made it difficult to efficiently model kidney diseases and development. Using polydimethylsiloxane (PDMS) scaffolds and a kidney-derived cell line we developed a system to rapidly engineer custom-made 3D tubules with typical renal epithelial properties. This system was successfully employed to engineer patient-specific tubules, to model polycystic kidney disease (PKD) and test drug efficacy, and to identify a po… Show more

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Cited by 31 publications
(28 citation statements)
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“…Other studies established in vitro differentiated single cell types, such as iREC ( in vitro differentiated renal epithelial cells) 29 and iPSC-derived podocytes 30 . Recently, engineered kidney tubules in polydimethylsilozan scaffold using iPSCs or cells from polycystic kidney disease (PKD) patient showed 3D tubules with renal epithelial properties 31 . The characterization and detailed comparisons of these cells and systems are still on-going.…”
Section: Discussionmentioning
confidence: 99%
“…Other studies established in vitro differentiated single cell types, such as iREC ( in vitro differentiated renal epithelial cells) 29 and iPSC-derived podocytes 30 . Recently, engineered kidney tubules in polydimethylsilozan scaffold using iPSCs or cells from polycystic kidney disease (PKD) patient showed 3D tubules with renal epithelial properties 31 . The characterization and detailed comparisons of these cells and systems are still on-going.…”
Section: Discussionmentioning
confidence: 99%
“…Moreover, bioengineering the structural and physiological features that are necessary for modeling specific aspects of the organ separately can significantly minimize the inherent variability of self-organizing systems and facilitate the development of robust human models for comparative and quantitative drug testing studies. Indeed, three-dimensional printed polydimethylsiloxane (PDMS) scaffolds have been successfully used to grow complex kidney tubules with predefined architectures with remarkable reproducibility ( Benedetti et al, 2018 ). This system was used to engineer patient-specific tubules, to model PKD and test drug efficacy, and to identify new therapeutic compounds for PKD.…”
Section: Key Technical Milestones To Be Achievedmentioning
confidence: 99%
“…The technology informing the generation of human kidney tissue de novo did not arise in one step; rather, the state of the art has been informed by insights acquired through studies performed during the past 6 decades. Some of these historical studies are listed in Table 1 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28Table 1Growing a new human kidneyaAuthors and reference nos.AchievementsOsathanondh and Potter4, 5, 6, 7Microdissection studies of normal and malformed human embryonic kidneysKeller et al.…”
Section: Question 1: Why Generate a New Human Kidney?mentioning
confidence: 99%
“… 26 ; Benedetti et al. 27 ; Czerniecki et al. 28 Using hPSC-derived kidney structures to model genetic kidney disease and test drug therapieshPSC, human pluripotent stem cell; 3D, 3-dimensional.aUnderpinning historical reports using native kidneys, as well as more recent studies using kidney tissues derived from pluripotent stem cells.…”
Section: Question 1: Why Generate a New Human Kidney?mentioning
confidence: 99%
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