Engineered M13 Peptide Carrier Promotes Angiogenic Potential of Patient-Derived Human Cardiac Progenitor Cells and In Vivo Engraftment

Jang, Woong Bi; Ji, Seung Taek; Park, Ji Hye; Kim, Yeon-Ju; Kang, Su-Tae; Kim, Da Yeon; Lee, Na-Kyung; Kim, Jin Su; Lim, Hye Ji; Choi, Jin-Ho; Le, Thi Hong Van; Ly, Thanh Truong Giang; Rethineswaran, Vinoth Kumar; Kim, Dong Hwan; Ha, Jong Seong; Yun, Jisoo; Baek, Sang Hong; Kwon, Sang‐Mo

doi:10.1007/s13770-020-00244-w

Cited by 9 publications

(13 citation statements)

References 48 publications

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“…One of the most extensively studied approaches is mesenchymal stem cell‐based therapy. [ 10,11 ] However, clinical trials have failed or shown at most marginally positive outcomes. [ 12 ] To promote resolution of post‐MI inflammation, adoptive cell transfer (ACT) of regulatory T cells (Tregs) or tolerogenic dendritic cells (tDCs) has been investigated.…”

Section: Introductionmentioning

confidence: 99%

Nanoparticle‐Mediated Blocking of Excessive Inflammation for Prevention of Heart Failure Following Myocardial Infarction

Kwon

Hwang

Park

et al. 2021

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Severe cardiac damage following myocardial infarction (MI) causes excessive inflammation, which sustains tissue damage and often induces adverse cardiac remodeling toward cardiac function impairment and heart failure. Timely resolution of post‐MI inflammation may prevent cardiac remodeling and development of heart failure. Cell therapy approaches for MI are time‐consuming and costly, and have shown marginal efficacy in clinical trials. Here, nanoparticles targeting the immune system to attenuate excessive inflammation in infarcted myocardium are presented. Liposomal nanoparticles loaded with MI antigens and rapamycin (L‐Ag/R) enable effective induction of tolerogenic dendritic cells presenting the antigens and subsequent induction of antigen‐specific regulatory T cells (Tregs). Impressively, intradermal injection of L‐Ag/R into acute MI mice attenuates inflammation in the myocardium by inducing Tregs and an inflammatory‐to‐reparative macrophage polarization, inhibits adverse cardiac remodeling, and improves cardiac function. Nanoparticle‐mediated blocking of excessive inflammation in infarcted myocardium may be an effective intervention to prevent the development of post‐MI heart failure.

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Section: Introductionmentioning

confidence: 99%

Nanoparticle‐Mediated Blocking of Excessive Inflammation for Prevention of Heart Failure Following Myocardial Infarction

Kwon

Hwang

Park

et al. 2021

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“…Up to now, nearly half of the known integrins have been found to bind to RGD sequences, promoting the development of biomaterial modification, effectively. For prolonging the use time of biomaterials and reducing the possibility of infection, more attention has been paid to the modification of RGD sequences , than cell adhesion proteins. Therefore, it is necessary to continue the development of RGD-containing composite polymer membranes for biomedicine, biomaterials, medical detection, etc.…”

Section: Introductionmentioning

confidence: 99%

Cell-Specific Adhesion Interfaces by Electrochemical Copolymerization of Arg-Gly-Asp-Tyr and 4-(2-(2-Ethoxyethoxy) Ethoxy) Phenol

Chen

et al. 2021

ACS Appl. Polym. Mater.

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Arg-Gly-Asp (RGD), a classical polypeptide, has been widely used in cell specific recognition. RGDY (Arg-Gly-Asp-Tyr) consists of the RGD sequence and tyrosine. Herein, a functional coating with cell-specific adhesion (with human umbilical vein endothelial cells as the model) and antiprotein adhesion (with bovine serum albumin as the model) properties was green synthesized by electrochemical copolymerization of RGDY and 4-(2-(2-ethoxyethoxy) ethoxy) phenol. Fourier transform infrared spectroscopy and scanning electron microscopy were used to characterize the functional groups and morphology of the copolymer, respectively. Cyclic voltammetry and electrochemical impedance spectroscopy were used to study the electrochemical performance of the modified electrode. A quartz crystal microbalance was used to reflect the modification and antiprotein adhesion process of the copolymer and the following process of cell-specific adhesion. The cell adsorption and antiprotein adhesion properties of the modified electrode are significantly improved. Herein, a green method was used to prepare a stable coating with cell-specific adhesion and antiprotein adhesion by electrochemical copolymerization of typical cell-specific recognition peptides and oligopoly(ethylene glycol) derivatives. It has a potential application value in the modification of biomaterials, the preparation of cell-specific sensors, and the development of special biological carriers.

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“…Tissue regeneration barely occurs without angiogenesis, which is the formation of new vessels from the pre-existing blood vessels and involves endothelial sprouting and splitting of vessel lumens [1,2]. Since insufficient blood supply is a major cause of several chronic diseases, numerous studies have concentrated on the technologies for advanced angiogenesis by using various strategies, such as scaffolds, hydrogels, cells, growth factors, and/or chemokines [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Nano-Sized Extracellular Matrix Particles Lead to Therapeutic Improvement for Cutaneous Wound and Hindlimb Ischemia

Kim

Savitri

et al. 2021

IJMS

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Cell-derived matrix (CDM) has proven its therapeutic potential and been utilized as a promising resource in tissue regeneration. In this study, we prepared a human fibroblast-derived matrix (FDM) by decellularization of in vitro cultured cells and transformed the FDM into a nano-sized suspended formulation (sFDM) using ultrasonication. The sFDM was then homogeneously mixed with Pluronic F127 and hyaluronic acid (HA), to effectively administer sFDM into target sites. Both sFDM and sFDM containing hydrogel (PH/sFDM) were characterized via immunofluorescence, sol–gel transition, rheological analysis, and biochemical factors array. We found that PH/sFDM hydrogel has biocompatible, mechanically stable, injectable properties and can be easily administered into the external and internal target regions. sFDM itself holds diverse bioactive molecules. Interestingly, sFDM-containing serum-free media helped maintain the metabolic activity of endothelial cells significantly better than those in serum-free condition. PH/sFDM also promoted vascular endothelial growth factor (VEGF) secretion from monocytes in vitro. Moreover, when we evaluated therapeutic effects of PH/sFDM via the murine full-thickness skin wound model, regenerative potential of PH/sFDM was supported by epidermal thickness, significantly more neovessel formation, and enhanced mature collagen deposition. The hindlimb ischemia model also found some therapeutic improvements, as assessed by accelerated blood reperfusion and substantially diminished necrosis and fibrosis in the gastrocnemius and tibialis muscles. Together, based on sFDM holding a strong therapeutic potential, our engineered hydrogel (PH/sFDM) should be a promising candidate in tissue engineering and regenerative medicine.

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Engineered M13 Peptide Carrier Promotes Angiogenic Potential of Patient-Derived Human Cardiac Progenitor Cells and In Vivo Engraftment

Cited by 9 publications

References 48 publications

Nanoparticle‐Mediated Blocking of Excessive Inflammation for Prevention of Heart Failure Following Myocardial Infarction

Nanoparticle‐Mediated Blocking of Excessive Inflammation for Prevention of Heart Failure Following Myocardial Infarction

Cell-Specific Adhesion Interfaces by Electrochemical Copolymerization of Arg-Gly-Asp-Tyr and 4-(2-(2-Ethoxyethoxy) Ethoxy) Phenol

Nano-Sized Extracellular Matrix Particles Lead to Therapeutic Improvement for Cutaneous Wound and Hindlimb Ischemia

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