Engineered Micro‐Objects as Scaffolding Elements in Cellular Building Blocks for Bottom‐Up Tissue Engineering Approaches

Leferink, Anne Marijke; Schipper, Dirk J.; Arts, E.; Vrij, E.J.; Rivron, Nicolas; Karperien, Marcel; Mittmann, K.; Blitterswijk, Clemens A. van; Moroni, Lorenzo; Truckenmüller, Roman

doi:10.1002/adma.201304539

Cited by 83 publications

(85 citation statements)

References 37 publications

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“…However, despite significant progress, a number of problems remain unsolved, among which vascularization is one of the foremost challenges4. For extensive bone defects that initially lack vascularization, if adequate vascular supply cannot be established in time, the implanted cells within graft interior would suffer from hypoxia and apoptosis, resulting in a necrotic core in the graft5. To solve this problem, numerous strategies have been developed, including the delivery of angiogenesis-promoting growth factors, co-culture with endothelial cells, genetic modification of seeded cells, and surgical pre-vascularization techniques6789.…”

mentioning

confidence: 99%

Extracellular Vesicle-functionalized Decalcified Bone Matrix Scaffolds with Enhanced Pro-angiogenic and Pro-bone Regeneration Activities

Xie

Wang

Zhang

et al. 2017

Sci Rep

130

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Vascularization is crucial for bone regeneration after the transplantation of tissue-engineered bone grafts in the clinical setting. Growing evidence suggests that mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) are potently pro-angiogenic both in vitro and in vivo. In the current study, we fabricated a novel EV-functionalized scaffold with enhanced pro-angiogenic and pro-bone regeneration activities by coating decalcified bone matrix (DBM) with MSC-derived EVs. EVs were harvested from rat bone marrow-derived MSCs and the pro-angiogenic potential of EVs was investigated in vitro. DBM scaffolds were then coated with EVs, and the modification was verified by scanning electron microscopy and confocal microscopy. Next, the pro-angiogenic and pro-bone regeneration activities of EV-modified scaffolds were evaluated in a subcutaneous bone formation model in nude mice. Micro-computed tomography scanning analysis showed that EV-modified scaffolds with seeded cells enhanced bone formation. Enhanced bone formation was confirmed by histological analysis. Immunohistochemical staining for CD31 proved that EV-modified scaffolds promoted vascularization in the grafts, thereby enhancing bone regeneration. This novel scaffold modification method provides a promising way to promote vascularization, which is essential for bone tissue engineering.

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mentioning

confidence: 99%

Extracellular Vesicle-functionalized Decalcified Bone Matrix Scaffolds with Enhanced Pro-angiogenic and Pro-bone Regeneration Activities

Xie

Wang

Zhang

et al. 2017

Sci Rep

130

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“…Microfabrication techniques based on micromolding, photo-patterning, and microfluidics enable the construction of the micro-building blocks of tissue constructs, such as cell-laden hydrogel particles, fibers, or mini-constructs of any shape [69]. To engineer micro-tissues, the next level of fabrication is needed, which is capable of organizing the micro-building blocks coherently and rationally into a tissue-like assembly.…”

Section: Spatial Control Of Hydrogelsmentioning

confidence: 99%

Spatially and temporally controlled hydrogels for tissue engineering

Leijten

Seo

Yue

et al. 2017

Materials Science and Engineering: R: Reports

159

129

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Summary Recent years have seen tremendous advances in the field of hydrogel-based biomaterials. One of the most prominent revolutions in this field has been the integration of elements or techniques that enable spatial and temporal control over hydrogels’ properties and functions. Here, we critically review the emerging progress of spatiotemporal control over biomaterial properties towards the development of functional engineered tissue constructs. Specifically, we will highlight the main advances in the spatial control of biomaterials, such as surface modification, microfabrication, photo-patterning, and three-dimensional (3D) bioprinting, as well as advances in the temporal control of biomaterials, such as controlled release of molecules, photocleaving of proteins, and controlled hydrogel degradation. We believe that the development and integration of these techniques will drive the engineering of next-generation engineered tissues.

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“…Macroscopic supramolecular assembly has attracted growing attention because it provides a promising strategy to fabricate structures in a bottom-up way for potential applications in electrical networks, 1 reinforced materials, 2 and tissue scaffolds. 3 To realize macroscopic supramolecular assembly, researchers have developed several kinds of driving forces to assemble macroscopic building blocks such as capillary interactions, 4 host-guest molecular recognition, 5 magnetic interactions, 6 electrostatic interaction, 7 and DNA hybridization. 8 In 2009, Harada's group reported the macroscopic assembly of soft hydrogels through molecular recognition of host-guest chemical groups.…”

Section: Introductionmentioning

confidence: 99%

Efficient modification with flexible spacing coating for in situ reversible assembly of semirigid macroscopic objects through hierarchical metal coordination

Akram

2017

Polymers for Advanced Techs

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Macroscopic supramolecular assembly plays a key role to bridge the fundamental researches on molecular recognition to the potential applications as supramolecular materials. However, the challenge remains to promote the research from soft hydrogel system to semirigid objects or building blocks. Herein, the concept of flexible spacing coating was employed to modify the model polydimethylsiloxane building blocks, and reversible macroscopic assembly was successfully realized through introducing highly directional, dynamic, and reversible coordinate interactions as driving forces. The driving force for the macroscopic assembly was confirmed by introducing highly competitive ethylene diamine tetra acetic acid solution as an orthodox system to disassemble the assembled blocks. Moreover, the coordinate interaction was further understood through unique in situ measurements of binding forces between building blocks during assembly process. This work of macroscopic supramolecular assembly provides an in situ visible platform, which is significant to clarify the highly fascinating and facile coordinate interactions on the macroscopic assembly behavior. To increase understanding of macroscopic assembly with flexible spacing coating, a fascinating noncovalent interaction that can be employed in macroscopic assembly is coordinate interactions. Selfassembly through coordinate interactions has emerged as a wellestablished methodology, and metal-ligand interactions have been employed in construction of metallosupramolecular structures very frequently. A variety of elaborate metallosupramolecules, from 2-dimensional polygons to 3-dimensional cages, prisms, and polyhedra,

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Engineered Micro‐Objects as Scaffolding Elements in Cellular Building Blocks for Bottom‐Up Tissue Engineering Approaches

Cited by 83 publications

References 37 publications

Extracellular Vesicle-functionalized Decalcified Bone Matrix Scaffolds with Enhanced Pro-angiogenic and Pro-bone Regeneration Activities

Extracellular Vesicle-functionalized Decalcified Bone Matrix Scaffolds with Enhanced Pro-angiogenic and Pro-bone Regeneration Activities

Spatially and temporally controlled hydrogels for tissue engineering

Efficient modification with flexible spacing coating for in situ reversible assembly of semirigid macroscopic objects through hierarchical metal coordination

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