Engineered Smooth Muscle Tissues: Regulating Cell Phenotype with the Scaffold

Kim, Byung Soo; Nikolovski, Janeta; Bonadio, Jeffrey; Smiley, Elizabeth; Mooney, David J.

doi:10.1006/excr.1999.4595

Cited by 187 publications

(114 citation statements)

References 45 publications

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“…These ndings suggest that TP(70)/gel(30) sca olds can provide a suitable substrate for SMCs, and physical adsorption of various biomolecules on their surface enables researchers to obtain a desirable proliferation rate. This nding, in accordance with those of other researchers [19][20][21], con rmed the behavioral regulation of the cells cultured on sca olds via the involvement of speci c integrin/ECM interaction. Additionally, as suggested by Kim et al, it was observed that the initial interaction of SMCs with the sca old can in uence the long-term behavior of the cells due to setting o a chain of signaling events through the initial cell contact with the sca old led to long-term changes in gene expression and tissue composition [21].…”

Section: Cell Growth and Morphologysupporting

confidence: 92%

Impact of electrospun Tecophilic/gelatin scaffold biofunctionalization on proliferation of vascular smooth muscle cells

2017

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Abstract. Nano brous composite sca olds based on Tecophilic (TP) and gelatin (gel) were electrospun, and further modi cation of their surfaces was performed by the adsorption of gelatin or bronectin biomolecules. The ability of coated sca olds to alter the proliferation rate of Smooth Muscle Cells (SMCs) was investigated via various assays and compared to cell proliferation on non-coated sca olds. The results con rmed the potential of both coated and non-coated composite sca olds to support SMC growth. Although the presence of bronectin increased the proliferation, adsorbed gelatin could reduce the proliferation of SMCs. The success of a tissue-engineered vascular graft depends on the ability of the sca old to control the proliferation rate of SMCs; thus, our study provides a better insight into the fabrication of functional constructs for vascular regeneration.

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Section: Cell Growth and Morphologysupporting

confidence: 92%

Impact of electrospun Tecophilic/gelatin scaffold biofunctionalization on proliferation of vascular smooth muscle cells

2017

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“…32 Expanded meniscal cells (passage number 2) were collected with 0.05% (w/v) trypsin-EDTA (Gibco BRL) in PBS, suspended in 10% (v/v) FBS-containing DMEM/F12 medium at a density of 10 7 cells/ml, and seeded onto the bonded scaffolds. Each meniscal scaffold was seeded with 2 ϫ 10 6 cells.…”

Section: Cell Seeding Onto Scaffoldsmentioning

confidence: 99%

Regeneration of whole meniscus using meniscal cells and polymer scaffolds in a rabbit total meniscectomy model

Kang

Son

Lee

et al. 2006

J Biomedical Materials Res

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Abstract:The current treatments of meniscal lesion in knee joint are not perfect to prevent adverse effects of meniscus injury. Tissue engineering of meniscus using meniscal cells and polymer scaffolds could be an alternative option to treat meniscus injury. This study reports on the regeneration of whole medial meniscus in a rabbit total meniscectomy model using the tissue engineering technique. Biodegradable scaffolds in a meniscal shape were fabricated from polyglycolic acid (PGA) fiber meshes that were mechanically reinforced by bonding PGA fibers at cross points with 75:25 poly(lactic-co-glycolic acid). The compressive modulus of the bonded PGA scaffold was 28-fold higher than that of nonbonded scaffold. Allogeneic meniscal cells were isolated from rabbit meniscus biopsy and cultured in vitro. The expanded meniscal cells were seeded onto the polymer scaffolds, cultured in vitro for 1 week, and transplanted to rabbit knee joints from which medial menisci were removed. Ten or 36 weeks after transplantation, the implants formed neomenisci with the original scaffold shape maintained approximately. Hematoxylin and eosin staining of the sections of the neomenisci at 6 and 10 weeks revealed the regeneration of fibrocartilage. Safranin-O staining showed that abundant proteoglycan was present in the neomenisci at 10 weeks. Masson's trichrome staining indicated the presence of collagen. Immunohistochemical analysis showed that the presence of type I and II collagen in neomenisci at 10 weeks was similar to that of normal meniscal tissue. Biochemical and biomechanical analyses of the tissue-engineered menisci at 36 weeks were performed to determine the quality of the tissue-engineered menisci. Tissue-engineered meniscus showed differences in collagen content and aggregate modulus in comparison with native meniscus. This study demonstrates, for the first time, the feasibility of regenerating whole meniscal cartilage in a rabbit total meniscectomy model using the tissue engineering method.

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“…56 While the materials will most directly control cells originally placed or recruited to the surface of the material, a number of reports indicate long-term control over both gene expression and tissue formation in vitro and in vivo, even though a small percentage of the cells is adherent to the material at those stages. 48,57 Inductive Factor Delivery Signaling between distinct cell populations clearly regulates many developmental processes, and cotransplantation of cell populations participating in this cross-talk can enhance tissue regeneration. 58 As an alternative to transplanting multiple cell populations, one can instead consider replacing their action by the delivery of the growth factors or morphogens underlying their bioactivity.…”

Section: Cell Instructive Polymers Adhesion Ligand Presentationmentioning

confidence: 99%

Regenerative medicine in orthopaedic surgery

Corsi

Schwarz

Mooney

et al. 2007

Journal Orthopaedic Research

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Regenerative medicine holds great promise for orthopaedic surgery. As surgeons continue to face challenges regarding the healing of diseased or injured musculoskeletal tissues, regenerative medicine aims to develop novel therapies that will replace, repair, or promote tissue regeneration. This review article will provide an overview of the different research areas involved in regenerative medicine, such as stem cells, bioinductive factors, and scaffolds. The potential use of stem cells for orthopaedic tissue engineering will be addressed by presenting the current progress with skeletal muscle-derived stem cells. As well, the development of a revascularized massive allograft will be described and will serve as a prototypic model of orthopaedic tissue engineering. Lastly, we will describe current approaches used to design cell instructive materials and how they can be used to promote and regulate the formation of bony tissue. ß

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Engineered Smooth Muscle Tissues: Regulating Cell Phenotype with the Scaffold

Cited by 187 publications

References 45 publications

Impact of electrospun Tecophilic/gelatin scaffold biofunctionalization on proliferation of vascular smooth muscle cells

Impact of electrospun Tecophilic/gelatin scaffold biofunctionalization on proliferation of vascular smooth muscle cells

Regeneration of whole meniscus using meniscal cells and polymer scaffolds in a rabbit total meniscectomy model

Regenerative medicine in orthopaedic surgery

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