Engineered substrates with imprinted cell-like topographies induce direct differentiation of adipose-derived mesenchymal stem cells into Schwann cells

Moghaddam, Mehrdad Moosazadeh; Bonakdar, Shahin; Shokrgozar, Mohammad Ali; Zaminy, Arash; Vali, Hojatollah; Faghihi, Shahab

doi:10.1080/21691401.2019.1586718

Cited by 35 publications

(30 citation statements)

References 55 publications

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“…Another approach involving biomechanical forces or micro-nano patterned topographical surfaces has demonstrated the feasibility of controlling the fate of the stem cells. MSC cultured on imprinted SC topographies results in the direct differentiation into SCLC [105]. On the other hand, combining a micro-patterned substrate with chemical induction does not improve the differentiation process.…”

Section: Physical-electrical Inductionmentioning

confidence: 99%

“…For example, shear stress is linked with ESC differentiation towards vascular endothelial cells, and the stretching of MSCs results in the up-regulation of smooth muscle cell markers [107,108]. Thus, the physical cues and structural features have gained increasing focus in the field, highlighting their important role in cell differentiation and transplantation ( Figure 1 and Table 1) [105,109]. MSCs were isolated for the first time from BM, and these cells were extensively studied for various applications, as recently reviewed by Gomez-Salazar et al in 2020 [110].…”

Section: Physical-electrical Inductionmentioning

confidence: 99%

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Schwann Cell-Like Cells: Origin and Usability for Repair and Regeneration of the Peripheral and Central Nervous System

Hopf

Schaefer

Kalbermatten

et al. 2020

Cells

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Functional recovery after neurotmesis, a complete transection of the nerve fiber, is often poor and requires a surgical procedure. Especially for longer gaps (>3 mm), end-to-end suturing of the proximal to the distal part is not possible, thus requiring nerve graft implantation. Artificial nerve grafts, i.e., hollow fibers, hydrogels, chitosan, collagen conduits, and decellularized scaffolds hold promise provided that these structures are populated with Schwann cells (SC) that are widely accepted to promote peripheral and spinal cord regeneration. However, these cells must be collected from the healthy peripheral nerves, resulting in significant time delay for treatment and undesired morbidities for the donors. Therefore, there is a clear need to explore the viable source of cells with a regenerative potential similar to SC. For this, we analyzed the literature for the generation of Schwann cell-like cells (SCLC) from stem cells of different origins (i.e., mesenchymal stem cells, pluripotent stem cells, and genetically programmed somatic cells) and compared their biological performance to promote axonal regeneration. Thus, the present review accounts for current developments in the field of SCLC differentiation, their applications in peripheral and central nervous system injury, and provides insights for future strategies.

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Section: Physical-electrical Inductionmentioning

confidence: 99%

Section: Physical-electrical Inductionmentioning

confidence: 99%

Schwann Cell-Like Cells: Origin and Usability for Repair and Regeneration of the Peripheral and Central Nervous System

Hopf

Schaefer

Kalbermatten

et al. 2020

Cells

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show abstract

“…MSCs differentiated on this substrate within 3 weeks showed specific morphology and expression of Schwannspecific markers (S100b, p75 NTR , and Sox10). 31 Thus, the synergic effect of nanoscale patterns and microscale order that helps to mimic the physiological cell microenvironment is one of the most promising approaches in tissue engineering.…”

Section: Matrix Properties Regulate Msc Behaviormentioning

confidence: 99%

Lineage Commitment, Signaling Pathways, and the Cytoskeleton Systems in Mesenchymal Stem Cells

Saidova

Vorobjev

2020

Tissue Engineering Part B: Reviews

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Mesenchymal stem cells (MSCs) from adult tissues are promising candidates for personalized cell therapy and tissue engineering. Significant progress was achieved in our understanding of the regulation of MSCs proliferation and differentiation by different cues during the past years. Proliferation and differentiation of MSCs are sensitive to the extracellular matrix (ECM) properties, physical cues, and chemical signaling. Sheath stress, matrix stiffness, surface adhesiveness, and micro-and nanotopography define cell shape and dictate lineage commitment of MSCs even in the absence of specific chemical signals. We discuss mechanotransduction as the major route from ECM through the cytoskeleton toward signaling pathways and gene expression. All components of the cytoskeleton from primary cilium and focal adhesions (FAs) to actin, microtubules (MTs), and intermediate filaments (IFs) are involved in the mechanotransduction. Differentiation of MSCs is regulated via the complex network of interrelated signaling pathways, including RhoA/ROCK, Akt/Erk, and YAP/TAZ effectors of Hippo pathway. These pathways could be regulated both by chemical and mechanical stimuli. Attenuation of these pathways in MSCs results in specific changes in FAs and actin cytoskeleton. Besides, differentiation of MSCs affects MTs and IFs. Recent findings highlight the role of intranuclear actin in the regulation of transcription factors in response to mechanical environmental stimuli. Alterations of cytoskeletal components reflect the MSC senescence state and their migratory capacity. In this review, we discuss the relationships between the molecular interactions in signaling pathways and morphological response of cytoskeletal components and reveal the complex interrelations between cytoskeleton systems and signaling pathways during lineage commitment of MSCs.

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“…Being dependent on traction forces, the arrangement of the adhesion ligands and organization of integrin binding can be modulated by matrix stiffness, in turn triggering the osteogenic commitment (Huebsch et al, 2010). Accordingly, tuning the physical properties of adhesion substrates has become a trend in manufacturing transplantable grafts with optimized biological features and enhanced regenerative potential (Moosazadeh Moghaddam et al, 2019). However, the nature and extent of biophysical phenomena underlying the ECM-mediated cellular fate modulation still require a deeper comprehension.…”

Section: Design Of Scaffolds For Bone Tissue Engineeringmentioning

confidence: 99%

Natural Polymeric Scaffolds in Bone Regeneration

Filippi

Born

Chaaban

et al. 2020

Front. Bioeng. Biotechnol.

272

151

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Despite considerable advances in microsurgical techniques over the past decades, bone tissue remains a challenging arena to obtain a satisfying functional and structural restoration after damage. Through the production of substituting materials mimicking the physical and biological properties of the healthy tissue, tissue engineering strategies address an urgent clinical need for therapeutic alternatives to bone autografts. By virtue of their structural versatility, polymers have a predominant role in generating the biodegradable matrices that hold the cells in situ to sustain the growth of new tissue until integration into the transplantation area (i.e., scaffolds). As compared to synthetic ones, polymers of natural origin generally present superior biocompatibility and bioactivity. Their assembly and further engineering give rise to a wide plethora of advanced supporting materials, accounting for systems based on hydrogels or scaffolds with either fibrous or porous architecture. The present review offers an overview of the various types of natural polymers currently adopted in bone tissue engineering, describing their manufacturing techniques and procedures of functionalization with active biomolecules, and listing the advantages and disadvantages in their respective use in order to critically compare their actual applicability potential. Their combination to other classes of materials (such as micro and nanomaterials) and other innovative strategies to reproduce physiological bone microenvironments in a more faithful way are also illustrated. The regeneration outcomes achieved in vitro and in vivo when the scaffolds are enriched with different cell types, as well as the preliminary clinical applications are presented, before the prospects in this research field are finally discussed. The collection of studies herein considered confirms that advances in natural polymer research will be determinant in designing translatable materials for efficient tissue regeneration with forthcoming impact expected in the treatment of bone defects.

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Engineered substrates with imprinted cell-like topographies induce direct differentiation of adipose-derived mesenchymal stem cells into Schwann cells

Abstract: View related articlesView Crossmark data Citing articles: 13 View citing articlesEngineered substrates with imprinted cell-like topographies induce direct differentiation of adipose-derived mesenchymal stem cells into Schwann cells

Cited by 35 publications

References 55 publications

Schwann Cell-Like Cells: Origin and Usability for Repair and Regeneration of the Peripheral and Central Nervous System

Schwann Cell-Like Cells: Origin and Usability for Repair and Regeneration of the Peripheral and Central Nervous System

Lineage Commitment, Signaling Pathways, and the Cytoskeleton Systems in Mesenchymal Stem Cells

Natural Polymeric Scaffolds in Bone Regeneration

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