Engineered Versions of the Wheat <i>Lr62</i> Translocation

Somo, Mohamed; Pirseyedi, Seyed Mostafa; Cai, Xiwen; Marais, Francois

doi:10.2135/cropsci2016.08.0683

Cited by 2 publications

(1 citation statement)

References 18 publications

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“…Four translocation recombinants that had the least amounts of Ae. neglecta chromatin and retained Lr62 but had lost Yr42 were studied further using SSR markers and GISH (Somo et al, 2017). It appeared that three of the latter recombined translocations were located at the 6AS telomere, whereas the fourth recombinant ( Lr62 ‐129), which was substantially shorter than the other three, had been relocated to 6BS.…”

Section: Characteristicsmentioning

confidence: 99%

Registration of Spring Wheat Sources of Leaf Rust Resistance Genes Lr53, Lr56, Lr59, and Lr62

Marais

McCallum

Kolmer

et al. 2017

J of Plant Registrations

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Spring wheat (Triticum aestivum L.) germplasm with the alien derived leaf rust (caused by Puccinia triticina Erikss.) resistance genes Lr53, Lr56, Lr59, and Lr62 has been developed over many years, initially at the University of Stellenbosch (South Africa), and then at the Cereal Research Centre (Agriculture and Agri‐food Canada), and North Dakota State University (USA). A Thatcher near‐isogenic line carrying Lr53, Thatcher‐Lr53 (Reg. No. GS‐185, PI 682091), was derived, while, following allosyndetic pairing induction, numerous recombinants of the translocations carrying Lr56, Lr59, and Lr62 have been produced and mapped. The shortest and potentially most useful recombinants were identified and named Lr56‐157 (Reg. No. GS‐186, PI 682092), Lr59‐151 (Reg. No. GS‐187, PI 682093) and Lr62‐129 (Reg. No. GS‐188, PI 682094). The four resistance genes may be homeo‐allelic since they have similar locations at the telomeric ends of either chromosome 6AS or 6BS, appear to give strong race‐specific resistance to a wide range of P. triticina races, occur in the proximity of the Xdupw217 simple sequence repeat marker locus, and, except for Lr59 (which is derived from a smaller primary introgression), were loosely associated with a stripe rust (caused by P. striiformis Westend. f. sp. tritici Erikss.) resistance locus in the original translocations. This study serves to confirm the potential effectiveness of the genes retained in the shortened translocations by testing with 11 representative, current US races of P. triticina.

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Section: Characteristicsmentioning

confidence: 99%

Registration of Spring Wheat Sources of Leaf Rust Resistance Genes Lr53, Lr56, Lr59, and Lr62

Marais

McCallum

Kolmer

et al. 2017

J of Plant Registrations

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Identification of leaf rust resistance loci in hard winter wheat using genome-wide association mapping

Lakkakula,

Kolmer,

Sharma

et al. 2024

Preprint

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Leaf rust, caused by Puccinia triticina (Pt), is a serious constraint to wheat production. Developing resistant varieties is the best approach to managing this disease. Wheat leaf rust resistance (Lr) genes have been classified into either all-stage resistance (ASR) or adult-plant resistance (APR). The objectives of this study were to identify sources of leaf rust resistance in contemporary U.S. hard winter wheat (HWW) and to dissect the genetic basis underlying leaf rust resistance in HWW. A panel of 732 elite HWW genotypes was evaluated for response to U.S. Pt races at the seedling stage and at the adult plant stage in leaf rust nurseries in Oklahoma, Texas, and Kansas. Further, the panel was genotyped using Multiplex Restriction Amplicon Sequencing (MRA-Seq) and DNA markers linked to the known ASR genes Lr18, Lr19, Lr21, Lr24, Lr37, and Lr42 and APR genes Lr34, Lr46, Lr67, Lr68, Lr77, and Lr78. Single nucleotide polymorphism (SNP) markers derived from MRA-Seq, DNA markers linked to the known Lr genes, and the phenotypic data were used for genome-wide association study (GWAS) to identify markers associated with leaf rust response. Gene postulation based on leaf rust reactions, DNA markers, and GWAS suggested the presence of Lr1, Lr2a, Lr10, Lr14a, Lr16, Lr18, Lr19, Lr21, Lr24, Lr26, Lr34, Lr37, Lr39, Lr42, Lr46, Lr68, Lr77, and Lr78 in the HWW panel. The GWAS identified 59 SNPs significantly associated with leaf rust response, of which 20 were likely associated with novel resistance loci and can be used to enhance wheat leaf rust resistance.

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Engineered Versions of the Wheat Lr62 Translocation

Cited by 2 publications

References 18 publications

Registration of Spring Wheat Sources of Leaf Rust Resistance Genes Lr53, Lr56, Lr59, and Lr62

Registration of Spring Wheat Sources of Leaf Rust Resistance Genes Lr53, Lr56, Lr59, and Lr62

Identification of leaf rust resistance loci in hard winter wheat using genome-wide association mapping

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