Engineered zinc oxide-based nanotherapeutics boost systemic antibacterial efficacy against phloem-restricted diseases

Soliman, Mikhael; Lee, Briana; Özcan, Ali; Rawal, Takat B.; Young, Mikaeel; Mendis, Hajeewaka C.; Rajasekaran, P.; Washington, Torus; Pingali, Sai Venkatesh; O’Neill, Hugh; Gesquiere, Andre J.; Fuente, Leonardo De La; Petridis, Loukas; Johnson, Evan G.; Graham, James H.; Santra, Santimukul; Tetard, Laurene

doi:10.1039/d2en00263a

Cited by 9 publications

(10 citation statements)

References 85 publications

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“…This study also demonstrated that nano-ZnO had higher mobility and bactericidal efficacy in sink reservoirs of the microfluidic chamber; therefore, it was more effective at clearing the biofilm compared to bulk ZnO. Soliman et al (2022) reported ZnO/ZnO 2 (2.7 nm core, 0.4 nm shell as determined via SANS measurements) nanotherapeutic formulations against HLB and canker-bearing citrus trees [ 249 ]. The authors discovered a significant decrease in canker lesions compared to Firewall ® control and in the HLB disease rating compared to Nordox ® 30/30 control.…”

Section: Triangular Management Strategiesmentioning

confidence: 81%

“…The authors discovered a significant decrease in canker lesions compared to Firewall ® control and in the HLB disease rating compared to Nordox ® 30/30 control. It must be also noted that the ZnO/ZnO 2 formulation caused a percentage increase in large fruit size yield compared to control, which is desired for the fresh fruit market [ 249 ]. Graham et al (2016) reported the efficacy of Zinkicidefor controlling the citrus canker of grapefruit, another important disease [ 250 ].…”

Section: Triangular Management Strategiesmentioning

confidence: 99%

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Huanglongbing Pandemic: Current Challenges and Emerging Management Strategies

Ghosh

Kokane

Savita

et al. 2022

Plants

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Huanglongbing (HLB, aka citrus greening), one of the most devastating diseases of citrus, has wreaked havoc on the global citrus industry in recent decades. The culprit behind such a gloomy scenario is the phloem-limited bacteria “Candidatus Liberibacter asiaticus” (CLas), which are transmitted via psyllid. To date, there are no effective long-termcommercialized control measures for HLB, making it increasingly difficult to prevent the disease spread. To combat HLB effectively, introduction of multipronged management strategies towards controlling CLas population within the phloem system is deemed necessary. This article presents a comprehensive review of up-to-date scientific information about HLB, including currently available management practices and unprecedented challenges associated with the disease control. Additionally, a triangular disease management approach has been introduced targeting pathogen, host, and vector. Pathogen-targeting approaches include (i) inhibition of important proteins of CLas, (ii) use of the most efficient antimicrobial or immunity-inducing compounds to suppress the growth of CLas, and (iii) use of tools to suppress or kill the CLas. Approaches for targeting the host include (i) improvement of the host immune system, (ii) effective use of transgenic variety to build the host’s resistance against CLas, and (iii) induction of systemic acquired resistance. Strategies for targeting the vector include (i) chemical and biological control and (ii) eradication of HLB-affected trees. Finally, a hypothetical model for integrated disease management has been discussed to mitigate the HLB pandemic.

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Section: Triangular Management Strategiesmentioning

confidence: 81%

Section: Triangular Management Strategiesmentioning

confidence: 99%

Huanglongbing Pandemic: Current Challenges and Emerging Management Strategies

Ghosh

Kokane

Savita

et al. 2022

Plants

Self Cite

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“…Etxeberria and Gonzalez applied Au, CuInS/ZnS, and CdSeS/ZnS and fluorescent polymeric NPs with sizes ranging from 1.4 to 15 nm into laser-cut incisions on a leaf from a citrus tree, and used fluorescence imaging to demonstrate the presence of those NPs in the phloem . Soliman et al applied 5 nm ZnO@ZnO 2 NPs on an exposed cut on a citrus plant leaf and observed the presence of Zn using EDS analyses in the leaf midrib that contains the vascular bundles, and specifically, Zn was found in both the phloem and xylem therein . However, it is important to note that the EDS signals do not distinguish between dissolved and particulate Zn.…”

Section: Resultsmentioning

confidence: 99%

“…Micronutrient deficiency in agricultural soils and poor uptake of applied micronutrients are reducing crop yields worldwide . Micronutrients are not only needed for crop productivity and quality but also to enhance the plant’s ability to withstand disease. − Optimizing the chemical speciation of micronutrients and their mode of delivery to crops can significantly improve crop yield and quality. − …”

Section: Introductionmentioning

confidence: 99%

Application of ZnO Nanoparticles Encapsulated in Mesoporous Silica on the Abaxial Side of a Solanum lycopersicum Leaf Enhances Zn Uptake and Translocation via the Phloem

Gao,

Kundu,

Persson

et al. 2023

Environ. Sci. Technol.

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Foliar application of nutrient nanoparticles (NPs) is a promising strategy for improving fertilization efficiency in agriculture. Phloem translocation of NPs from leaves is required for efficient fertilization but is currently considered to be feasible only for NPs smaller than a cell wall pore size exclusion limit of <20 nm. Using mass spectrometry imaging, we provide here the first direct evidence for phloem localization and translocation of a larger (∼70 nm) fertilizer NP comprised of ZnO encapsulated in mesoporous SiO 2 (ZnO@MSN) following foliar deposition. The Si content in the phloem tissue of the petiole connected to the dosed leaf was ∼10 times higher than in the xylem tissue, and ∼100 times higher than the phloem tissue of an untreated tomato plant petiole. Direct evidence of NPs in individual phloem cells has only previously been shown for smaller NPs introduced invasively in the plant. Furthermore, we show that uptake and translocation of the NPs can be enhanced by their application on the abaxial (lower) side of the leaf. Applying ZnO@MSN to the abaxial side of a single leaf resulted in a 56% higher uptake of Zn as well as higher translocation to the younger (upper) leaves and to the roots, than dosing the adaxial (top) side of a leaf. The higher abaxial uptake of NPs is in alignment with the higher stomatal density and lower density of mesophyll tissues on that side and has not been demonstrated before.

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“…19 Due to the limited effectiveness and longstanding use of copper biocides in agriculture, coppertolerant strains have emerged in several pathogenic bacterial species. 17,[20][21][22] To manage these copper-tolerant pathogens several alternatives such as magnesium, [23][24][25] sulphur, [26][27][28][29] and transition metal-based [30][31][32][33][34][35][36] nanomaterials have been identified to conventional copper-based biocides. These alternatives possess have demonstrated plant compatibility, biological efficacy, and economical feasibility.…”

Section: Introductionmentioning

confidence: 99%

Targeted delivery of oxytetracycline to the epidermal cell junction and stomata for crop protection

Pereira,

Negrete Moreno,

Gan Giannelli

et al. 2023

Environ. Sci.: Nano

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Engineered zinc oxide-based nanotherapeutics boost systemic antibacterial efficacy against phloem-restricted diseases

Abstract: Defect engineering at the surface of zinc oxide sub-5 nm nanoparticles provides a systemic nanotherapeutic with significant field efficacy against citrus HLB disease.

Cited by 9 publications

References 85 publications

Huanglongbing Pandemic: Current Challenges and Emerging Management Strategies

Huanglongbing Pandemic: Current Challenges and Emerging Management Strategies

Application of ZnO Nanoparticles Encapsulated in Mesoporous Silica on the Abaxial Side of a Solanum lycopersicum Leaf Enhances Zn Uptake and Translocation via the Phloem

Targeted delivery of oxytetracycline to the epidermal cell junction and stomata for crop protection

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