2021
DOI: 10.1016/j.foodchem.2021.129462
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Engineering a carbohydrate binding module to enhance chitinase catalytic efficiency on insoluble chitinous substrate

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Cited by 18 publications
(8 citation statements)
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“…coli DH5α cells. Positive transformants were selected on kanamycin Luria–Bertani (LB) plates, confirmed by PCR and subsequently DNA sequencing. Plasmids containing the coding sequences were extracted and transformed into E.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…coli DH5α cells. Positive transformants were selected on kanamycin Luria–Bertani (LB) plates, confirmed by PCR and subsequently DNA sequencing. Plasmids containing the coding sequences were extracted and transformed into E.…”
Section: Methodsmentioning
confidence: 99%
“…The enzyme activity was measured using the DNS method with a minor modification . The specific activity of chitosanase was determined with a reaction mixture consisting of 30.9 nM purified enzyme with 0.5% (w/v) colloidal chitosan solution in 50 mM sodium acetate buffer, pH 6.0.…”
Section: Methodsmentioning
confidence: 99%
“…Wang et al [73] increased the activity of chitinase BcChiA1 by 16.89 times through directed evolution, and also realized its high expression in Bacillus subtilis to produce CHOS. Su et al [74] replaced the original chitinase binding domain with a chitin-binding module from Bacillus circulans WL-12, thus increasing the chitinase activity by 49%. In addition, random mutation can also effectively improve the activity of chitinase.…”
Section: Enzymatic Methodsmentioning
confidence: 99%
“…The challenges of high complexity and high pretreatment costs can be overcome if chitinase can directly hydrolyze chitin powders at industrial rates. Compared with the synergistic effect of ChBD ChiA1 in the original chitinase Al, engineered ChBD produced excellent effects on insoluble chitin substrates, which was a very important step towards large-scale processing of chitin and had great economic benefits [ 161 ]. The fermentation medium of fecal bacteria, Alca F2018, proposed that the recycling of RSC to produce chitosan can ignore the purification step and obtain chitosan with high yield and sufficient degree of deacetylation and low crystallinity, which is an environmentally friendly process.…”
Section: Concluding Remarks and Future Directionsmentioning
confidence: 99%