Engineering a morphogenetically active hydrogel for bioprinting of bioartificial tissue derived from human osteoblast-like SaOS-2 cells

Neufurth, Meik; Wang, Xiaohong; Schröder, Heinz C.; Feng, Qingling; Diehl‐Seifert, Bärbel; Ziebart, Thomas; Steffen, Renate; Wang, Shunfeng; Müller, Wernér E.G.

doi:10.1016/j.biomaterials.2014.07.002

Cited by 167 publications

(135 citation statements)

References 55 publications

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“…Alginate has shown particular relevance as a bioink due to its compatibility with cells, ease in forming cross-linked hydrogels, and the ability to control biodegradation. Khalil and Sun demonstrate bioprinting of 3D tissue constructs using alginate and endothelial cells [117] and alginate stabilized with gelatin was a suitable matrix for 3D bioprinting of bone-related SaOS-2 cells [118,119]. Common to these reports is a high (>80%) cell viability following bioprinting.…”

Section: 3d Cell Culturementioning

confidence: 99%

3D Cell Culture in Alginate Hydrogels

2015

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This review compiles information regarding the use of alginate, and in particular alginate hydrogels, in culturing cells in 3D. Knowledge of alginate chemical structure and functionality are shown to be important parameters in design of alginate-based matrices for cell culture. Gel elasticity as well as hydrogel stability can be impacted by the type of alginate used, its concentration, the choice of gelation technique (ionic or covalent), and divalent cation chosen as the gel inducing ion. The use of peptide-coupled alginate can control cell–matrix interactions. Gelation of alginate with concomitant immobilization of cells can take various forms. Droplets or beads have been utilized since the 1980s for immobilizing cells. Newer matrices such as macroporous scaffolds are now entering the 3D cell culture product market. Finally, delayed gelling, injectable, alginate systems show utility in the translation of in vitro cell culture to in vivo tissue engineering applications. Alginate has a history and a future in 3D cell culture. Historically, cells were encapsulated in alginate droplets cross-linked with calcium for the development of artificial organs. Now, several commercial products based on alginate are being used as 3D cell culture systems that also demonstrate the possibility of replacing or regenerating tissue.

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Section: 3d Cell Culturementioning

confidence: 99%

3D Cell Culture in Alginate Hydrogels

2015

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“…Frequently used bio‐inks such as gelatin,5 alginate,6 gelatin/alginate,4 gelatin/alginate/chitosan,3 and poly(ethylene glycol) (PEG) dimethacrylate/gelatin7 that have been used in the fabrication of cell‐laden scaffolds have shown high biocompatibility for cell viability in bone repair. However, they lack osteogenic capability to promote cell differentiation and new bone formation in the absence of growth factors, which represents a clear limitation for their successful application.…”

Section: Introductionmentioning

confidence: 99%

3D‐Bioprinted Osteoblast‐Laden Nanocomposite Hydrogel Constructs with Induced Microenvironments Promote Cell Viability, Differentiation, and Osteogenesis both In Vitro and In Vivo

et al. 2017

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An osteoblast‐laden nanocomposite hydrogel construct, based on polyethylene glycol diacrylate (PEGDA)/laponite XLG nanoclay ([Mg5.34Li0.66Si8O20(OH)4]Na0.66, clay)/hyaluronic acid sodium salt (HA) bio‐inks, is developed by a two‐channel 3D bioprinting method. The novel biodegradable bio‐ink A, comprised of a poly(ethylene glycol) (PEG)–clay nanocomposite crosslinked hydrogel, is used to facilitate 3D‐bioprinting and enables the efficient delivery of oxygen and nutrients to growing cells. HA with encapsulated primary rat osteoblasts (ROBs) is applied as bio‐ink B with a view to improving cell viability, distribution uniformity, and deposition efficiency. The cell‐laden PEG–clay constructs not only encapsulated osteoblasts with more than 95% viability in the short term but also exhibited excellent osteogenic ability in the long term, due to the release of bioactive ions (magnesium ions, Mg2+ and silicon ions, Si4+), which induces the suitable microenvironment to promote the differentiation of the loaded exogenous ROBs, both in vitro and in vivo. This 3D‐bioprinting method holds much promise for bone tissue regeneration in terms of cell engraftment, survival, and ultimately long‐term function.

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“…This approach has already been used by several groups to assess the mechanical properties of tissues and cells. 11,[31][32][33][34][35][36][37][38] Furthermore, in 2013, we have demonstrated that the ferrule-top probes used for indentation can be modi¯ed to host another optical ber that, connected with an OCT system, enables the user to look at how the subsurface features of a sample deform when the indenter is pushed into the sample. 39 Those studies have never been brought beyond a¯rst proof-of-concept, whose practical relevance in life sciences was very limited: the use of new fabrication techniques and materials lead to a reduction of the sti®ness of the cantilever by three orders of magnitude with respect to those initial studies, enabling measurements on biologicallyrelevant soft materials.…”

Section: Introductionmentioning

confidence: 99%

Multimodal probe for optical coherence tomography epidetection and micron-scale indentation

Bartolini

Feroldi

Weda

et al. 2017

J. Innov. Opt. Health Sci.

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We present a multimodal ferrule-top sensor designed to perform the integrated epidetection of Optical Coherence Tomography (OCT) depth-pro¯les and micron-scale indentation by all-optical detection. By scanning a sample under the probe, we can obtain structural cross-section images and identify a region-of-interest in a nonhomogeneous sample. Then, with the same probe and setup, we can immediately target that area with a series of spherical-indentation measurements, in which the applied load is known with a N precision, the indentation depth with sub-m precision and a maximum contact radius of 100 m. Thanks to the visualization of the internal structure of the sample, we can gain a better insight into the observed mechanical behavior. The ability to impart a small, con¯ned load, and perform OCT A-scans at the same time, could lead to an alternative, high transverse resolution, Optical Coherence Elastography (OCE) sensor.

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Engineering a morphogenetically active hydrogel for bioprinting of bioartificial tissue derived from human osteoblast-like SaOS-2 cells

Cited by 167 publications

References 55 publications

3D Cell Culture in Alginate Hydrogels

3D Cell Culture in Alginate Hydrogels

3D‐Bioprinted Osteoblast‐Laden Nanocomposite Hydrogel Constructs with Induced Microenvironments Promote Cell Viability, Differentiation, and Osteogenesis both In Vitro and In Vivo

Multimodal probe for optical coherence tomography epidetection and micron-scale indentation

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