Engineering a SERS Sensing Nanoplatform with Self-Sterilization for Undifferentiated and Rapid Detection of Bacteria

Cao, Jian; Zhu, Wei; Zhou, Ji; Zhao, Bin; Pan, Yao-Yu; Ye, Yong; Shen, Aiguo

doi:10.3390/bios13010075

Cited by 2 publications

(2 citation statements)

References 35 publications

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“…Moreover, SERS is not only free from the interference of water [ 27 ] but also causes no damage to test samples [ 28 , 29 ], making it very suitable for analyzing biological samples [ 30 ], such as bacteria [ 31 ], viruses [ 32 , 33 ] and biomarkers in blood or body fluids [ 34 ]. SERS applied for bacteria detection has great advantages, such as a fast detection speed and high sensitivity without using labeling agents such as antibodies, and, in particular, it has simultaneous detection capabilities for multiple types of bacteria [ 35 , 36 ]. In addition, SERS is considered a promising tool to detect bacteria in infected wounds or blood tissue without damaging the actual sample structure [ 37 , 38 ].…”

Section: Introductionmentioning

confidence: 99%

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Recent Progress of Surface-Enhanced Raman Spectroscopy for Bacteria Detection

Liu

Wang

et al. 2023

Biosensors

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There are various pathogenic bacteria in the surrounding living environment, which not only pose a great threat to human health but also bring huge losses to economic development. Conventional methods for bacteria detection are usually time-consuming, complicated and labor-intensive, and cannot meet the growing demands for on-site and rapid analyses. Sensitive, rapid and effective methods for pathogenic bacteria detection are necessary for environmental monitoring, food safety and infectious bacteria diagnosis. Recently, benefiting from its advantages of rapidity and high sensitivity, surface-enhanced Raman spectroscopy (SERS) has attracted significant attention in the field of bacteria detection and identification as well as drug susceptibility testing. Here, we comprehensively reviewed the latest advances in SERS technology in the field of bacteria analysis. Firstly, the mechanism of SERS detection and the fabrication of the SERS substrate were briefly introduced. Secondly, the label-free SERS applied for the identification of bacteria species was summarized in detail. Thirdly, various SERS tags for the high-sensitivity detection of bacteria were also discussed. Moreover, we emphasized the application prospects of microfluidic SERS chips in antimicrobial susceptibility testing (AST). In the end, we gave an outlook on the future development and trends of SERS in point-of-care diagnoses of bacterial infections.

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Section: Introductionmentioning

confidence: 99%

“…In recent years, SERS has blossomed into a rapidly growing research area for the detection of various kinds of bacteria [ 36 , 39 , 40 ]. Bacteria detection based on SERS can be mainly divided into two categories: the label-free method [ 34 ] and the label-based method using SERS tags [ 41 ].…”

Section: Introductionmentioning

confidence: 99%

Recent Progress of Surface-Enhanced Raman Spectroscopy for Bacteria Detection

Liu

Wang

et al. 2023

Biosensors

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Fabrication of Aluminum Foil Integrated Pegylated Gold Nanoparticle Surface-Enhanced Raman Scattering Substrate for the Detection and Classification of Uropathogenic Bacteria

Yadav,

NaziaTarannum

2024

ACS Appl. Bio Mater.

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Rapid detection and classification of pathogenic microbes for food hygiene, healthcare, environmental contamination, and chemical and biological exposures remain a major challenge due to nonavailability of fast and accurate detection methods. The delay in clinical diagnosis of the most frequent bacterial infections, particularly urinary tract infections (UTIs), which affect about half of the population at least once in their lifetime, can be fatal if not detected and treated appropriately. In this work, we have fabricated aluminum (Al) foil integrated pegylated gold nanoparticles (AuNPs) as a potential surface-enhanced Raman scattering (SERS) substrate, which is used for the detection and classification of uropathogens, namely, E. coli, S. aureus, and P. aeruginosa directly from the culture without any pretreatment. The substrate is first drop cast with bacterial pellets and then pegylated AuNPs, and the interaction of two on Al foil base gives identifiable characteristic Raman peaks with good reproducibility. With the use of chemometric methods such as principal component analysis (PCA), the Al foil-based SERS substrate offers a quick, effective detection and classification of three strains of UTI bacteria with the least bacterial concentration (105 cells mL–1) necessary for clinical diagnosis. In addition, this substrate was able to detect E. coli positive clinical samples by giving SERS fingerprint information directly from centrifuged urine samples within minutes. The stability of pegylated AuNPs provides for its application at the point of care with rapid and easy detection of uropathogens as well as the possibility of advancement in healthcare applications.

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Engineering a SERS Sensing Nanoplatform with Self-Sterilization for Undifferentiated and Rapid Detection of Bacteria

Cited by 2 publications

References 35 publications

Recent Progress of Surface-Enhanced Raman Spectroscopy for Bacteria Detection

Recent Progress of Surface-Enhanced Raman Spectroscopy for Bacteria Detection

Fabrication of Aluminum Foil Integrated Pegylated Gold Nanoparticle Surface-Enhanced Raman Scattering Substrate for the Detection and Classification of Uropathogenic Bacteria

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