Engineering an artificial alveolar-capillary membrane: a novel continuously perfused model within microchannels

Nalayanda, Divya D.; Wang, Qihong; Fulton, William B.; Wang, Tza Huei

doi:10.1016/j.jpedsurg.2009.10.008

Cited by 37 publications

(30 citation statements)

References 11 publications

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“…To this end, several strategies have been used in an attempt to enhance vascularization in TE muscle. The first method utilizes bioengineered scaffolds that have been designed to incorporate “channels” for the supply of nutrients to the regenerating tissue [38,39]. In a second approach, angiogenic growth factors are linked to a scaffold in order to entice the infiltration of host ECs into the regenerating tissue [40,41].…”

Section: Discussionmentioning

confidence: 99%

The role of endothelial cells in myofiber differentiation and the vascularization and innervation of bioengineered muscle tissue in vivo

et al. 2013

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Musculoskeletal disorders are a major cause of disability and effective treatments are currently lacking. Tissue engineering affords the possibility of new therapies utilizing cells and biomaterials for the recovery of muscle volume and function. A major consideration in skeletal muscle engineering is the integration of a functional vasculature within the regenerating tissue. In this study we employed fluorescent cell labels to track the location and differentiation of co-cultured cells in vivo and in vitro. We first utilized a co-culture of fluorescently labeled endothelial cells (ECs) and muscle progenitor cells (MPCs) to investigate the ability of ECs to enhance muscle tissue formation and vascularization in an in vivo model of bioengineered muscle. Scaffolds that had been seeded with both MPCs and ECs showed significantly greater vascularization, tissue formation and enhanced innervation as compared to scaffolds seeded with MPCs alone. Subsequently, we performed in vitro experiments using a 3-cell type system (ECs, MPCs, and pericytes (PCs)) to demonstrate the utility of fluorescent cell labeling for monitoring cell growth and differentiation. The growth and differentiation of individual cell types was determined using live cell fluorescent microscopy demonstrating the utility of fluorescent labels to monitor tissue organization in real time.

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Section: Discussionmentioning

confidence: 99%

The role of endothelial cells in myofiber differentiation and the vascularization and innervation of bioengineered muscle tissue in vivo

et al. 2013

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“…The system also allowed for testing of cellular responses to mechanical changes in sheer stress by altering the flow rate through the channels. This revealed that cells exposed to high flow rates and thus higher sheer stress levels had decreased proliferation when compared with cells exposed to low flow (Nalayanda et al 2010). Carraro and colleagues used MEMS photolithographic and etching protocols to create a patterned template that can be used to mould silicone wafers with a vascular-type branching pattern.…”

Section: Nanoscale Surface Patterningmentioning

confidence: 96%

Engineering extracellular matrix through nanotechnology

Kelleher

Vacanti

2010

J. R. Soc. Interface.

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The goal of tissue engineering is the creation of a living device that can restore, maintain or improve tissue function. Behind this goal is a new idea that has emerged from twentieth century medicine, science and engineering. It is preceded by centuries of human repair and replacement with non-living materials adapted to restore function and cosmetic appearance to patients whose tissues have been destroyed by disease, trauma or congenital abnormality. The nineteenth century advanced replacement and repair strategies based on moving living structures from a site of normal tissue into a site of defects created by the same processes. Donor skin into burn wounds, tendon transfers, intestinal replacements into the urinary tract, toes to replace fingers are all examples. The most radical application is that of vital organ transplantation in which a vital part such as heart, lung or liver is removed from one donor, preserved for transfer and implanted into a patient dying of end-stage organ failure. Tissue engineering and regenerative medicine have advanced a general strategy combining the cellular elements of living tissue with sophisticated biomaterials to produce living structures of sufficient size and function to improve patients' lives. Multiple strategies have evolved and the application of nanotechnology can only improve the field. In our era, by necessity, any medical advance must be successfully commercialized to allow widespread application to help the greatest number of patients. It follows that business models and regulatory agencies must adapt and change to enable these new technologies to emerge. This brief review will discuss the science of nanotechnology and how it has been applied to this evolving field. We will then briefly summarize the history of commercialization of tissue engineering and suggest that nanotechnology may be of use in breeching the barriers to commercialization although its primary mission is to improve the technology by solving some remaining and vexing problems in its science and engineering aspects.

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“…For a comprehensive overview on such models, readers are referred to the recent t 4 report by Gordon and colleagues (2015). Until recently, only a few MPS trying to emulate functional parts of the lung have been developed (Nalayanda et al, 2007; Nalayanda et al, 2010), but in 2010, a microphysiological lung-on-a-chip system developed by Donald Ingber’s group at the Wyss Institute for Biologically Inspired Engineering at Harvard University Boston, MA, USA, made it for the first time into Science Magazine (Huh et al, 2010). Figure 7a shows the design and principles of this system which is mimicking the function of a lung alveola.…”

Section: Microphysiological Systems – An Expanding Toolbox For Hazmentioning

confidence: 99%

Biology-inspired microphysiological system approaches to solve the prediction dilemma of substance testing

Marx¹,

Andersson

Bahinski

et al. 2016

ALTEX

193

229

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Summary The recent advent of microphysiological systems – microfluidic biomimetic devices that aspire to emulate the biology of human tissues, organs and circulation in vitro – is envisaged to enable a global paradigm shift in drug development. An extraordinary US governmental initiative and various dedicated research programs in Europe and Asia have led recently to the first cutting-edge achievements of human single-organ and multi-organ engineering based on microphysiological systems. The expectation is that test systems established on this basis would model various disease stages, and predict toxicity, immunogenicity, ADME profiles and treatment efficacy prior to clinical testing. Consequently, this technology could significantly affect the way drug substances are developed in the future. Furthermore, microphysiological system-based assays may revolutionize our current global programs of prioritization of hazard characterization for any new substances to be used, for example, in agriculture, food, ecosystems or cosmetics, thus, replacing laboratory animal models used currently. Thirty-five experts from academia, industry and regulatory bodies present here the results of an intensive workshop (held in June 2015, Berlin, Germany). They review the status quo of microphysiological systems available today against industry needs, and assess the broad variety of approaches with fit-for-purpose potential in the drug development cycle. Feasible technical solutions to reach the next levels of human biology in vitro are proposed. Furthermore, key organ-on-a-chip case studies, as well as various national and international programs are highlighted. Finally, a roadmap into the future is outlined, to allow for more predictive and regulatory-accepted substance testing on a global scale.

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Engineering an artificial alveolar-capillary membrane: a novel continuously perfused model within microchannels

Cited by 37 publications

References 11 publications

The role of endothelial cells in myofiber differentiation and the vascularization and innervation of bioengineered muscle tissue in vivo

The role of endothelial cells in myofiber differentiation and the vascularization and innervation of bioengineered muscle tissue in vivo

Engineering extracellular matrix through nanotechnology

Biology-inspired microphysiological system approaches to solve the prediction dilemma of substance testing

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