Engineering an efficient whole‐cell catalyst for d‐allulose production from glycerol

Abstract

d‐Allulose has many health‐benefiting properties and therefore sustainably applied in food, pharmaceutical, and nutrition industries. The aldol reaction‐based route is a very promising alternative to Izumoring strategy in d‐allulose production. Remarkable studies reported in the past cannot get rid of by‐product formation and costly purified enzyme usage. In the present study, we explored the glycerol assimilation by modularly assembling the d‐allulose synthetic cascade in Escherichia coli envelop. We achieved an efficient whole‐cell catalyst that produces only d‐allulose from cheap glycerol feedstock, eliminating the involvement of purified enzymes. Detailed process optimization improved the d‐allulose titer by 1500.00%. Finally, the production was validated in 3‐L scale using a 5‐L fermenter, and 5.67 g L−1 d‐allulose was produced with a molar yield of 31.43%.

Production of D‐tagatose, bioethanol, and microbial protein from the dairy industry by‐product whey powder using an integrated bioprocess

“…[25,26] However, the isomerase reaction had a lower conversion rate because of the unfavorable thermodynamic equilibria. [8,9,27] Therefore, an oxidoreductive pathway was selected for tagatose synthesis in this study.…”

CYP116B5‐SOX: An artificial peroxygenase for drug metabolites production and bioremediation

“…In order to study the applicability of the fusion enzyme catalysis in bioremediation we performed a reaction scale-up exploiting a strain of E. coli BL21 (DE3) cells transformed with the pET-28-a-CYP116B5-SOX(+) vector: E. coli (CYP116B5-SOX). We employed the transformed bacteria as biocatalytic system to remove tamoxifen, [64][65][66][67][68][69][70][71][72][73] commonly reported as water pollutant, [9] from an aqueous buffered medium, taken as a model of contaminated water. We used HPLC-MS to identify the metabolites produced by CYP116B5-SOX catalysis.…”

Enhanced Biosynthesis of d-Allulose from a d-Xylose–Methanol Mixture and Its Self-Inductive Detoxification by Using Antisense RNAs in Escherichia coli

“…1 It is regarded as a commendable sweetener because of its 70% sucrose-like sweetness and fairly low caloric content. 2,3 Furthermore, research has substantiated the noteworthy effectiveness of Dallulose in hypolipidemic action, obesity prevention, and neuroprotection. 4−6 Enzymatic catalysis for D-allulose production has several advantages, including high substrate conversion, rapid reaction rate, and facile product purification.…”

“…d -Allulose, a naturally rare six-carbon sugar, is present in vegetables and fruits at extremely low levels . It is regarded as a commendable sweetener because of its 70% sucrose-like sweetness and fairly low caloric content. , Furthermore, research has substantiated the noteworthy effectiveness of d -allulose in hypolipidemic action, obesity prevention, and neuroprotection. − Enzymatic catalysis for d -allulose production has several advantages, including high substrate conversion, rapid reaction rate, and facile product purification. , However, the manufacturing and immobilization of enzymes significantly contribute to the overall economic burden . In contrast, fermentation may be a promising alternative that combines enzyme production and d -allulose synthesis within a single bioreactor …”