Engineering anisotropic human stem cell-derived three-dimensional cardiac tissue on-a-chip

Veldhuizen, Jaimeson; Cutts, Joshua; Brafman, David A.; Migrino, Raymond Q.; Nikkhah, Mehdi

doi:10.1016/j.biomaterials.2020.120195

Cited by 59 publications

(72 citation statements)

References 64 publications

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“…Incorporating staggered microposts into microdevices induced alignment in 3-D rat-and human-derived cardiac tissues. The aligned tissues exhibited enhanced mature gene expression profiles, cell structure, and tissue function in anisotropic tissues relative to tissues formed in the absence of the microposts (99). Microdevices also facilitate the self-assembly of 3-D tissues.…”

Section: -D Approaches and Micro-/nanofibers In Oocsmentioning

confidence: 99%

Biomimetic microsystems for cardiovascular studies

Inbody

Sinquefield

Lewis

et al. 2021

American Journal of Physiology-Cell Physiology

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Tissue culture platforms have been around for several decades and have enabled numerous key findings in the cardiovascular field. However, these platforms fail to recreate the mechanical and dynamic features found within the body. Organs-on-chips (OOCs) are cellularized microfluidic based devices that can mimic the basic structure, function, and responses of organs. These systems have been successfully utilized in disease, development, and drug studies. OOCs are designed to recapitulate the mechanical, electrical, chemical, and structural features of the in vivo microenvironment. Here, we review cardiovascular-themed OOC studies, design considerations, and techniques used to generate microtissues within these devices. Further, we will highlight the advantages of OOCs over traditional cell culture methods, discuss implementation challenges, and provide perspectives on the state of the field.

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Section: -D Approaches and Micro-/nanofibers In Oocsmentioning

confidence: 99%

Biomimetic microsystems for cardiovascular studies

Inbody

Sinquefield

Lewis

et al. 2021

American Journal of Physiology-Cell Physiology

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“…Apart from continuous grooves and ridges, where cells can grow and spread along with them [ 92 , 109 , 117 , 118 ], distributed pillars and posts can also form anisotropic structures to guide axon outgrowth [ 113 , [119] , [120] , [121] ]. Simitzi et al fabricated micro-cones on Si wafers using a pulsed laser [ 49 ].…”

Section: Anisotropic Surface Patternsmentioning

confidence: 99%

Anisotropic scaffolds for peripheral nerve and spinal cord regeneration

Xue

Shi

Kong

et al. 2021

Bioactive Materials

112

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The treatment of long-gap (>10 mm) peripheral nerve injury (PNI) and spinal cord injury (SCI) remains a continuous challenge due to limited native tissue regeneration capabilities. The current clinical strategy of using autografts for PNI suffers from a source shortage, while the pharmacological treatment for SCI presents dissatisfactory results. Tissue engineering, as an alternative, is a promising approach for regenerating peripheral nerves and spinal cords. Through providing a beneficial environment, a scaffold is the primary element in tissue engineering. In particular, scaffolds with anisotropic structures resembling the native extracellular matrix (ECM) can effectively guide neural outgrowth and reconnection. In this review, the anatomy of peripheral nerves and spinal cords, as well as current clinical treatments for PNI and SCI, is first summarized. An overview of the critical components in peripheral nerve and spinal cord tissue engineering and the current status of regeneration approaches are also discussed. Recent advances in the fabrication of anisotropic surface patterns, aligned fibrous substrates, and 3D hydrogel scaffolds, as well as their in vitro and in vivo effects are highlighted. Finally, we summarize potential mechanisms underlying the anisotropic architectures in orienting axonal and glial cell growth, along with their challenges and prospects.

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“…For example, Veldhuizen et al created a platform where they cocultured a 4:1 ratio of CMs and CFs within a hydrogel housed inside a microfluidic device. [ 26 ] The use of staggered microposts formed an anisotropic structure which may also affect the tissue function and cell maturation. After 2 weeks in culture, the cardiac tissue exhibited synchronous contractions, well‐defined sarcomeric striations, and the upregulated several maturation genes such as HCN1 and KCNQ1, which is typical for fully matured CMs.…”

Section: Currently Available Cardiac Platformsmentioning

confidence: 99%

“…Therefore, several strategies must be implemented to hasten the maturity of these cells, including long-term in vitro culture, [23] electrical and mechanical stimulation, [24] 3D cultures, [25] and cocultures with other cell types. [26] For example, Kamakura et al performed year-long cultures of their hiPSC-CMs where they observed the formation Figure 1. The five major design considerations for engineered heart models.…”

Section: Doi: 101002/anbr202000022mentioning

confidence: 99%

“…Therefore, several strategies must be implemented to hasten the maturity of these cells, including long‐term in vitro culture, [ 23 ] electrical and mechanical stimulation, [ 24 ] 3D cultures, [ 25 ] and cocultures with other cell types. [ 26 ] For example, Kamakura et al performed year‐long cultures of their hiPSC‐CMs where they observed the formation of Z and I bands in as little as 30 days. [ 23 ] Rather than performing long‐term cultures, Nunes et al applied electrical stimulation to their culture and found an increase in surface area and sarcomere organization, which is typical in adult CMs [24b]…”

Section: Design Considerations For In Vitro Cardiac Platformsmentioning

confidence: 99%

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Designing Biomaterial Platforms for Cardiac Tissue and Disease Modeling

House¹,

Atalla²,

Lee³

et al. 2020

Advanced NanoBiomed Research

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Heart disease is one of the leading causes of death in the world. There is a growing demand for in vitro cardiac models that can recapitulate the complex physiology of the cardiac tissue. These cardiac models can provide a platform to better understand the underlying mechanisms of cardiac development and disease and aid in developing novel treatment alternatives and platforms toward personalized medicine. Herein, a summary of engineered cardiac platforms is presented. Basic design considerations for replicating the heart's microenvironment are discussed considering the anatomy of the heart. This is followed by a detailed summary of the currently available biomaterial platforms for modeling the heart tissue in vitro. These in vitro models include 2D surface modified structures, 3D molded structures, porous scaffolds, electrospun scaffolds, bioprinted structures, and heart‐on‐a‐chip devices. The challenges faced by current models and the future directions of in vitro cardiac models are also discussed. Engineered in vitro tissue models utilizing patients’ own cells can potentially revolutionize the way we develop treatment and diagnostic alternatives.

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Engineering anisotropic human stem cell-derived three-dimensional cardiac tissue on-a-chip

Cited by 59 publications

References 64 publications

Biomimetic microsystems for cardiovascular studies

Biomimetic microsystems for cardiovascular studies

Anisotropic scaffolds for peripheral nerve and spinal cord regeneration

Designing Biomaterial Platforms for Cardiac Tissue and Disease Modeling

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